Selection and control efficiency of Trichoderma isolates against Fusarium oxysporum f. sp. ciceris in Iran

2021 ◽  
pp. 101731
Author(s):  
Hassan Younesi ◽  
Eidi Bazgir ◽  
Mostafa Darvishnia ◽  
Khosrow Chehri
Keyword(s):  
Fusarium Oxysporum ◽  
Control Efficiency ◽  
And Control ◽  
Trichoderma Isolates

scholarly journals Detection and Control of Fusarium oxysporum from Soft Rot in Dendrobium officinale by Loop-Mediated Isothermal Amplification Assays

Biology ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1136
Author(s):  
Caiyun Xiao ◽  
Rongyu Li
Keyword(s):  
Fusarium Oxysporum ◽  
Integrated Management ◽  
Elongation Factor ◽  
Lamp Assay ◽  
Soft Rot ◽  
Isothermal Amplification ◽  
Dendrobium Officinale ◽  
Control Effect ◽  
Loop Mediated Isothermal Amplification ◽  
And Control

Soft rot causing Fusarium oxysporum is one of the most destructive diseases of Dendrobium officinale Kimura et Migo in China that reduces D. officinale yield and quality. A key challenge for an integrated management strategy for this disease is the rapid and accurate detection of F. oxysporum on D. officinale. Therefore, a new loop-mediated isothermal amplification (LAMP) assay was developed for this purpose. In this study, the primers were selected and designed using the translation elongation factor-1α (TEF-1α) gene region as the target DNA sequence in order to screen the best system of reaction of LAMP to detect F. oxysporum through optimizing different conditions of the LAMP reaction, including time, temperature, concentrations of MgSO4, and concentrations of inner and outer primers. The optimized system was able to efficiently amplify the target gene at 62 °C for 60 min with 1.2 μM internal primers, 0.4 μM external primers, 7 mM Mg2+, and 5 fg/µL minimum detection concentration of DNA for F. oxysporum. The amplified products could be detected with the naked eye after completion of the reaction with SYBR green I. We were better able to control the effect of soft rot in D. officinale using fungicides following a positive test result. Additionally, the control effect of synergism combinations against soft rot was higher than 75%. Thus, LAMP assays could detect F. oxysporum in infected tissues of D. officinale and soils in field, allowing for early diagnosis of the disease.

CONSEQUENCES AND CONTROL OF LATENT FUSARIUM OXYSPORUM INFECTIONS IN TULIP BULBS

1980 ◽  
pp. 381-386
Author(s):  
B.H.H. Bergman ◽  
M. A.M. Bakker-van der Voort
Keyword(s):  
Fusarium Oxysporum ◽  
Tulip Bulbs ◽  
And Control

scholarly journals Fusarium Wilt: A New Threat to Florida Lettuce Production

EDIS ◽  
2020 ◽  
Vol 2020 (5) ◽  
Author(s):  
German Sandoya ◽  
Jesse J. Murray ◽  
Richard N. Raid ◽  
Christian F. Miller
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Control Measures ◽  
Agricultural Area ◽  
Disease Symptoms ◽  
Everglades Agricultural Area ◽  
Production Areas ◽  
And Control

Fusarium wilt of lettuce is a disease caused by the fungus Fusarium oxysporum f.sp. lactucae. The disease is present in all lettuce production areas worldwide. Recently this disease has been identified in Florida in localized fields. This new 4-page publication of the UF/IFAS Horticultural Sciences Department presents information about the disease, symptoms, and control measures useful to lettuce growers in Florida. Following these recommendations may help to avoid the spread of this fungus in muck soils at the Everglades Agricultural Area (EAA), where 90% of the lettuce in Florida is planted. Written by Germán V. Sandoya, Jesse J. Murray, Richard N. Raid, and Christian F. Miller.https://edis.ifas.ufl.edu/hs1385

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