Development of a sequence-characterized amplified region marker for detection of Ascochyta rabiei causing Ascochyta blight in chickpea

2019 ◽  
Vol 65 (1) ◽  
pp. 103-108
Author(s):  
Mathew S. Baite ◽  
Balendu K. Upadhyay ◽  
Sunil C. Dubey
Keyword(s):  
Ascochyta Blight ◽  
Ascochyta Rabiei ◽  
Sequence Characterized Amplified Region

scholarly journals Reference Genome Assembly for Australian Ascochyta rabiei Isolate ArME14

2020 ◽  
Vol 10 (7) ◽  
pp. 2131-2140
Author(s):  
Ramisah Mohd Shah ◽  
Angela H. Williams ◽  
James K. Hane ◽  
Julie A. Lawrence ◽  
Lina M. Farfan-Caceres ◽  
...  
Keyword(s):  
Secondary Metabolite ◽  
Dna Sequences ◽  
Genome Assembly ◽  
Ascochyta Blight ◽  
Ascochyta Rabiei ◽  
Causal Organism ◽  
Protein Coding ◽  
Putative Protein ◽  
Genomic Regions ◽  
Genome Assemblies

Ascochyta rabiei is the causal organism of ascochyta blight of chickpea and is present in chickpea crops worldwide. Here we report the release of a high-quality PacBio genome assembly for the Australian A. rabiei isolate ArME14. We compare the ArME14 genome assembly with an Illumina assembly for Indian A. rabiei isolate, ArD2. The ArME14 assembly has gapless sequences for nine chromosomes with telomere sequences at both ends and 13 large contig sequences that extend to one telomere. The total length of the ArME14 assembly was 40,927,385 bp, which was 6.26 Mb longer than the ArD2 assembly. Division of the genome by OcculterCut into GC-balanced and AT-dominant segments reveals 21% of the genome contains gene-sparse, AT-rich isochores. Transposable elements and repetitive DNA sequences in the ArME14 assembly made up 15% of the genome. A total of 11,257 protein-coding genes were predicted compared with 10,596 for ArD2. Many of the predicted genes missing from the ArD2 assembly were in genomic regions adjacent to AT-rich sequence. We compared the complement of predicted transcription factors and secreted proteins for the two A. rabiei genome assemblies and found that the isolates contain almost the same set of proteins. The small number of differences could represent real differences in the gene complement between isolates or possibly result from the different sequencing methods used. Prediction pipelines were applied for carbohydrate-active enzymes, secondary metabolite clusters and putative protein effectors. We predict that ArME14 contains between 450 and 650 CAZymes, 39 putative protein effectors and 26 secondary metabolite clusters.

scholarly journals Identification of novel sources of resistance to ascochyta blight in a collection of wild Cicer accessions

2020 ◽  
Author(s):  
Toby E. Newman ◽  
Silke Jacques ◽  
Christy Grime ◽  
Fiona L. Kamphuis ◽  
Robert C. Lee ◽  
...  
Keyword(s):  
Genome Wide Association Study ◽  
Ascochyta Blight ◽  
Ascochyta Rabiei ◽  
Sources Of Resistance ◽  
Highly Pathogenic ◽  
Cicer Echinospermum ◽  
Genome Wide ◽  
A Genome ◽  
The Impact ◽  
Chickpea Cultivars

Chickpea production is constrained worldwide by the necrotrophic fungal pathogen Ascochyta rabiei, the causal agent of ascochyta blight (AB). In order to reduce the impact of this disease, novel sources of resistance are required in chickpea cultivars. Here, we screened a new collection of wild Cicer accessions for AB resistance and identified accessions resistant to multiple, highly pathogenic isolates. In addition to this, analyses demonstrated that some collection sites of Cicer echinospermum harbour predominantly resistant accessions, knowledge that can inform future collection missions. Furthermore, a genome-wide association study identified regions of the Cicer reticulatum genome associated with AB resistance and investigation of these regions identified candidate resistance genes. Taken together, these results can be utilised to enhance the resistance of chickpea cultivars to this globally yield-limiting disease.

CDC Corinne desi chickpea

2009 ◽  
Vol 89 (3) ◽  
pp. 515-516 ◽  
Author(s):  
B. Taran ◽  
T. Warkentin ◽  
S. Banniza ◽  
A. Vandenberg
Keyword(s):  
Cicer Arietinum ◽  
Ascochyta Blight ◽  
Yield Potential ◽  
Ascochyta Rabiei ◽  
Western Canada ◽  
Canadian Prairies ◽  
Cicer Arietinum L ◽  
Crop Development ◽  
Cultivar Description ◽  
Release Program

CDC Corinne, a desi chickpea (Cicer arietinum L.) cultivar, was released in 2008 by the Crop Development Centre, University of Saskatchewan, for distribution to Select seed growers in western Canada through the Variety Release Program of the Saskatchewan Pulse Growers. CDC Corinne has a pinnate leaf type, fair resistance to ascochyta blight [Ascochyta rabiei (Pass.) Lab.], medium maturity, medium seed size and higher yield potential than Myles in the Brown and Dark Brown soil zones of the Canadian prairies. Key words: Chickpea, Cicer arietinum L., cultivar description, ascochyta blight

Development of new kabuli large-seeded chickpea materials with resistance to Ascochyta blight

2017 ◽  
Vol 68 (11) ◽  
pp. 967 ◽  
Author(s):  
J. Gil ◽  
P. Castro ◽  
T. Millan ◽  
E. Madrid ◽  
J. Rubio
Keyword(s):  
Seed Size ◽  
Mediterranean Basin ◽  
Consumer Preferences ◽  
Ascochyta Blight ◽  
Ascochyta Rabiei ◽  
Key Factors ◽  
Cicer Arietinum L ◽  
Large Seed ◽  
Serious Disease ◽  
The Mediterranean Basin

Appearance and size of chickpea (Cicer arietinum L.) seeds are key factors for the market in the Mediterranean Basin driven by consumer preferences. Hence, kabuli large seeds are sold on the market at higher price than the desi seeds. In this crop, Ascochyta blight (caused by Ascochyta rabiei (Pass.) Lab.) is a serious disease causing major losses in yield. Thus, developing large-seeded kabuli cultivars resistant to blight would be of great importance to farmers. In this study, the use of transgressive inheritance to select new allelic combinations for seed size was applied to develop new chickpea materials with large seeds and resistance to blight. Crosses between five different advanced lines of kabuli chickpea genotypes with medium–large seed size and resistant to blight were performed. As a results of the selections carried out during 10 successive years, 11 F5:9 lines resistant to blight and with large seed size were selected to be released as future varieties. The markers SCY17590 and CaETR were employed to confirm blight resistance of the material developed.

The effect of carrier volume on ascochyta blight (Ascochyta rabiei) control in chickpea

2008 ◽  
Vol 27 (6) ◽  
pp. 1020-1030 ◽  
Author(s):  
C. Armstrong-Cho ◽  
T. Wolf ◽  
G. Chongo ◽  
Y. Gan ◽  
T. Hogg ◽  
...  
Keyword(s):  
Ascochyta Blight ◽  
Ascochyta Rabiei ◽  
Carrier Volume

Ascochyta blight: isolation, characterization, and development of a rapid method to detect inhibitors of the chickpea fungal pathogen Ascochyta rabiei

2016 ◽  
Vol 120 (3) ◽  
pp. 424-432 ◽  
Author(s):  
Luciana Bahr ◽  
María Victoria Castelli ◽  
Melisa Isabel Barolo ◽  
Nathalie Ruiz Mostacero ◽  
María Elena Tosello ◽  
...  
Keyword(s):  
Rapid Method ◽  
Fungal Pathogen ◽  
Ascochyta Blight ◽  
Ascochyta Rabiei

Genetic mapping of ascochyta blight resistance in chickpea (Cicer arietinum L.) using a simple sequence repeat linkage map

Genome ◽  
2007 ◽  
Vol 50 (1) ◽  
pp. 26-34 ◽  
Author(s):  
B. Tar’an ◽  
T.D. Warkentin ◽  
A. Tullu ◽  
A. Vandenberg
Keyword(s):  
Linkage Map ◽  
Cicer Arietinum ◽  
Phenotypic Variation ◽  
Simple Sequence Repeat ◽  
Ascochyta Blight ◽  
Ascochyta Rabiei ◽  
Blight Resistance ◽  
Sequence Repeat ◽  
Cicer Arietinum L ◽  
Simple Sequence

Ascochyta blight, caused by the fungus Ascochyta rabiei (Pass.) Lab., is one of the most devastating diseases of chickpea ( Cicer arietinum L.) worldwide. Research was conducted to map genetic factors for resistance to ascochyta blight using a linkage map constructed with 144 simple sequence repeat markers and 1 morphological marker (fc, flower colour). Stem cutting was used to vegetatively propagate 186 F2 plants derived from a cross between Cicer arietinum L. ‘ICCV96029’ and ‘CDC Frontier’. A total of 556 cutting-derived plants were evaluated for their reaction to ascochyta blight under controlled conditions. Disease reaction of the F1 and F2 plants demonstrated that the resistance was dominantly inherited. A Fain’s test based on the means and variances of the ascochyta blight reaction of the F3 families showed that a few genes were segregating in the population. Composite interval mapping identified 3 genomic regions that were associated with the reaction to ascochyta blight. One quantitative trait locus (QTL) on each of LG3, LG4, and LG6 accounted for 13%, 29%, and 12%, respectively, of the total estimated phenotypic variation for the reaction to ascochyta blight. Together, these loci controlled 56% of the total estimated phenotypic variation. The QTL on LG4 and LG6 were in common with the previously reported QTL for ascochyta blight resistance, whereas the QTL on LG3 was unique to the current population.

Sensitivity of field populations of Ascochyta rabiei to chlorothalonil, mancozeb and pyraclostrobin fungicides and effect of strobilurin fungicides on the progress of ascochyta blight of chickpea

2007 ◽  
Vol 87 (4) ◽  
pp. 937-944 ◽  
Author(s):  
K. F. Chang ◽  
H. U. Ahmed ◽  
S. F. Hwang ◽  
B. D. Gossen ◽  
S. E. Strelkov ◽  
...  
Keyword(s):  
Ascochyta Blight ◽  
Ascochyta Rabiei ◽  
Growth Media ◽  
Single Spore ◽  
Fungicide Application ◽  
Strobilurin Fungicides ◽  
Cultural Measures ◽  
Fungicide Tolerance ◽  
Total Crop ◽  
Fungicide Insensitivity

Chickpea production faces a major challenge from ascochyta blight (Ascochyta rabiei), a devastating disease that can cause total crop loss. To assess the effect of repeated fungicide application on disease progress, strobilurin fungicides, primarily alternating pyraclostrobin and azoxystrobin treatments, were applied up to five times per year in each of 2 yr. A single application or two early applications reduced blight severity. A third application resulted in additional benefits in 1 of 2 yr, but additional applications did not reduce severity further. To monitor for fungicide tolerance in populations of A. rabiei, 66 single- spore isolates were collected and grown on growth media amended with chlorothalonil, mancozeb, or pyraclostrobin. Insensitivity to one or more of the fungicides was detected in 49 (74%) of the isolates. Based on the effect on conidial germination, insensitivity to pyraclostrobin or chlorothalonil was observed in 26 of 37 isolates (70%). Repeated fungicide application may be selecting for insensitive isolates of the pathogen; fungicide application should be combined with cultural measures to control ascochyta blight. Key words: Fungicide insensitivity, Ascochyta rabiei

scholarly journals Ascochyta blight of chickpea (Cicer arietinum L.): a review of biology, pathogenicity, and disease management

2005 ◽  
Vol 56 (4) ◽  
pp. 317 ◽  
Author(s):  
S. Pande ◽  
K. H. M. Siddique ◽  
G. K. Kishore ◽  
B. Bayaa ◽  
P. M. Gaur ◽  
...  
Keyword(s):  
Cicer Arietinum ◽  
Foliar Application ◽  
Ascochyta Blight ◽  
Management Strategies ◽  
Hydrolytic Enzymes ◽  
Research Priorities ◽  
Ascochyta Rabiei ◽  
Future Research ◽  
Management Options ◽  
Cicer Arietinum L

Ascochyta blight (AB), caused by Ascochyta rabiei is a major disease of chickpea (Cicer arietinum L.), especially in areas where cool, cloudy, and humid weather persists during the crop season. Several epidemics of AB causing complete yield loss have been reported. The fungus mainly survives between seasons through infected seed and in infected crop debris. Despite extensive pathological and molecular studies, the nature and extent of pathogenic variability in A. rabiei have not been clearly established. Accumulation of phenols, phytoalexins (medicarpin and maackiain), and hydrolytic enzymes has been associated with host-plant resistance (HPR). Seed treatment and foliar application of fungicides are commonly recommended for AB management, but further information on biology and survival of A. rabiei is needed to devise more effective management strategies. Recent studies on inheritance of AB resistance indicate that several quantitative trait loci (QTLs) control resistance. In this paper we review the biology of A. rabiei, HPR, and management options, with an emphasis on future research priorities.

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