Identification of GIMAP7 and Rabl3 as Putative Biomarkers for Oral Squamous Cell Carcinoma Through Comparative Proteomic Approach

2019 ◽  
Vol 26 (3) ◽  
pp. 1817-1822
Author(s):  
Muhammad Usman ◽  
Amber Ilyas ◽  
Zehra Hashim ◽  
Shamshad Zarina
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Proteomic Approach

S025 Biomarkers of Invasiveness in Oral Squamous Cell Carcinoma: A Cell Proteomic Approach

2006 ◽  
Vol 132 (8) ◽  
pp. 844
Author(s):  
A. M. Mlynarek ◽  
R. L. Balys ◽  
M. P. Hier ◽  
M. J. Black ◽  
M. A. Alaoui-Jamali
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Proteomic Approach ◽  
A Cell

Identification of RAB2A and PRDX1 as the potential biomarkers for oral squamous cell carcinoma using mass spectrometry-based comparative proteomic approach

Tumor Biology ◽  
2015 ◽  
Vol 36 (12) ◽  
pp. 9829-9837 ◽  
Author(s):  
Kaushik Kumar Dey ◽  
Ipsita Pal ◽  
Rashmi Bharti ◽  
Goutam Dey ◽  
B. N. Prashanth Kumar ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Proteomic Approach ◽  
Potential Biomarkers

scholarly journals A Cell Proteomic Approach for the Detection of Secretable Biomarkers of Invasiveness in Oral Squamous Cell Carcinoma

2007 ◽  
Vol 133 (9) ◽  
pp. 910 ◽  
Author(s):  
Alex M. Mlynarek ◽  
Richard L. Balys ◽  
Jie Su ◽  
Michael P. Hier ◽  
Martin J. Black ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Proteomic Approach ◽  
A Cell

A concerted cell and serum proteomic approach for the identification of oral squamous cell carcinoma biomarkers

2006 ◽  
Vol 24 (18_suppl) ◽  
pp. 5512-5512
Author(s):  
A. M. Mlynarek ◽  
R. Balys ◽  
S. Jie ◽  
Y. Xu ◽  
M. P. Hier ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Cancer ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cell Lines ◽  
Squamous Cell ◽  
Serum Biomarkers ◽  
Mouse Serum ◽  
Proteomic Approach ◽  
Albumin Depletion

5512 Background: Head and neck squamous cell carcinoma is the sixth most common cause of cancer deaths in the world. Despite extensive research, the 5-year survival rates have not changed significantly over the last decade. Presently, the lack of serum biomarkers for head and neck carcinoma limits early diagnosis and treatment monitoring of advanced disease. In this study, we used a proteomic approach on serum from mice bearing human oral squamous cell carcinoma xenografts and from conditioned cell culture medium from matched cell lines. Methods: Oral squamous cell carcinomas, with distinct invasive phenotypes, and adjacent normal tissue were obtained from human patients, and were transplanted orthotopicaly into tongues of RAG-2/γ(c) mice. After a 20% weight loss, the mice were sacrificed by exsanguinations. Two distinct proteomic protocols were used to analyze the mouse serum. The first involved albumin depletion followed by two-dimensional gel electrophoresis and MALDI. The second arm utilized the same albumin depletion followed by multidimensional-protein-information-technology (ESI-LC and MS/MS). The top candidate proteins, which were differentially expressed in the cancer bearing mice compared to matched controls were then validated by Western blot, immunoprecipitation, immunofluorescence, and/or immunohistochemistry analyses using mouse serum and conditioned medium from matched cell lines. Results: Orthotopic implantation of human tumor tissues in mouse tongue was successful in 100% of the mice tested. The human origin of these tumors was confirmed pathologically. A correlation between disease stages and invasiveness was observed. We identified over one hundred proteins as being differentially expressed between control and cancer-bearing mice (p < 0.05), including proteins involved in cell cytoskeleton signaling. The expression of these proteins was then validated in mouse serum, tissue xenografts, and conditioned medium from oral cancer matched established cell lines. Conclusion: We report the first proteomic in-vivo model of oral cancer for the identification of low molecular weight serum biomarkers. We identified candidates that can be exploited as potential markers for diagnosis of human oral squamous cell carcinoma. No significant financial relationships to disclose.

Role of Clinical Palpation in Evaluation of Cervical Lymph Node in Oral Squamous Cell Carcinoma

2013 ◽  
Vol 1 (2) ◽  
pp. 02-06
Author(s):  
SM Anwar Sadat ◽  
Sufia Nasrin Rita ◽  
Shoma Banik ◽  
Md Nazmul Hasan Khandker ◽  
Md Mahfuz Hossain ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Lymph Node ◽  
Oral Squamous Cell Carcinoma ◽  
Lymph Nodes ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Predictive Value ◽  
Cervical Lymph Node ◽  
Betel Quid ◽  
Cervical Lymph Nodes

A cross sectional study of 29 cases of oral squamous cell carcinoma with or without  cervical lymph node metastasis was done among Bangladeshi patients from January 2006 to December 2007. Majority of the study subjects (34.5%) belonged to the age group of 40-49 years. 58.6% of the study subjects were male, while remaining 41.4% of them were female. 51.7% of the lesions were located in the alveolar ridge where the other common sites were buccal mucosa (27.6%) and retro molar area (13.8%). Half of the study subjects (51.7%) were habituated to betel quid chewing followed by 37.9% and 10.3% were habituated to smoking and betel quid-smoking respectively. Grade I lesions was most prevalent (75.9%) in the study subjects.  Majority of cases presented with Stage IV lesions (55.2%). The sensitivity, specificity, positive predictive value, negative predictive value & accuracy of clinical palpation method for determining metastatic cervical lymph nodes were 93.33%, 64.29%, 73.68%, 90% and 79.3% respectively. Careful and repeated clinical palpation plays important role in evaluation of cervical lymph nodes though several modern techniques may help additionally in the management of oral cancer.DOI: http://dx.doi.org/10.3329/updcj.v1i2.13978 Update Dent. Coll. j. 2011: 1(2): 02-06

Management of Neck in Oral Squamous Cell Carcinoma

2011 ◽  
Vol 3 (6) ◽  
pp. 419-422
Author(s):  
Dr. Shool Rohit S Dr. Shool Rohit S ◽  
◽  
Dr. Anand P Zingade ◽  
Dr. Manish Kumar
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell

Evaluation of Proliferative Activity in Oral Squamous Cell Carcinoma by the AgNOR Staining Method

Odonto ◽  
2011 ◽  
Vol 19 (38) ◽  
pp. 115-121
Author(s):  
S.O. Silva ◽  
K.P. Gatto ◽  
J.P. De Carli ◽  
P.H.C. Souza ◽  
C.S. Busin
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Proliferative Activity ◽  
Staining Method ◽  
Agnor Staining
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