Pilot-scale production of carboxymethylcellulase from rice hull by Bacillus amyloliquefaciens DL-3

2008 ◽  
Vol 13 (2) ◽  
pp. 182-188 ◽  
Author(s):  
Kang-Ik Jo ◽  
You-Jung Lee ◽  
Bo-Kyung Kim ◽  
Bo-Hwa Lee ◽  
Chung-Han Chung ◽  
...  
Keyword(s):  
Bacillus Amyloliquefaciens ◽  
Pilot Scale ◽  
Rice Hull ◽  
Scale Production ◽  
Pilot Scale Production

scholarly journals Pilot-Scale Production of Chito-Oligosaccharides Using an Innovative Recombinant Chitosanase Preparation Approach

Polymers ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 290
Author(s):  
Chih-Yu Cheng ◽  
Chia-Huang Tsai ◽  
Pei-Jyun Liou ◽  
Chi-Hang Wang
Keyword(s):  
Cell Density ◽  
Scale Up ◽  
Cost Effective ◽  
Pilot Scale ◽  
Ionic Concentration ◽  
Dihydrogen Phosphate ◽  
Scale Production ◽  
Sodium Dihydrogen Phosphate ◽  
Selective Precipitation ◽  
Pilot Scale Production

For pilot-scale production of chito-oligosaccharides, it must be cost-effective to prepare designable recombinant chitosanase. Herein, an efficient method for preparing recombinant Bacillus chitosanase from Escherichia coli by elimination of undesirable substances as a precipitate is proposed. After an optimized culture with IPTG (Isopropyl β-d-1-thiogalactopyranoside) induction, the harvested cells were resuspended, disrupted by sonication, divided by selective precipitation, and stored using the same solution conditions. Several factors involved in these procedures, including ion types, ionic concentration, pH, and bacterial cell density, were examined. The optimal conditions were inferred to be pH = 4.5, 300 mM sodium dihydrogen phosphate, and cell density below 1011 cells/mL. Finally, recombinant chitosanase was purified to >70% homogeneity with an activity recovery and enzyme yield of 90% and 106 mg/L, respectively. When 10 L of 5% chitosan was hydrolyzed with 2500 units of chitosanase at ambient temperature for 72 h, hydrolyzed products having molar masses of 833 ± 222 g/mol with multiple degrees of polymerization (chito-dimer to tetramer) were obtained. This work provided an economical and eco-friendly preparation of recombinant chitosanase to scale up the hydrolysis of chitosan towards tailored oligosaccharides in the near future.

Pilot-scale production of cloudy juice from low-quality pear fruit under low-oxygen conditions

2015 ◽  
Vol 173 ◽  
pp. 827-837 ◽  
Author(s):  
Domien De Paepe ◽  
Katleen Coudijzer ◽  
Bart Noten ◽  
Dirk Valkenborg ◽  
Kelly Servaes ◽  
...  
Keyword(s):  
Pilot Scale ◽  
Scale Production ◽  
Low Oxygen ◽  
Pear Fruit ◽  
Pilot Scale Production ◽  
Oxygen Conditions

scholarly journals Ethyl biodiesels derived from non-edible oils within the biorefinery concept – Pilot scale production & engine emissions

2017 ◽  
Vol 109 ◽  
pp. 634-645 ◽  
Author(s):  
Svitlana Nitièma-Yefanova ◽  
Valérie Tschamber ◽  
Romain Richard ◽  
Sophie Thiebaud-Roux ◽  
Brice Bouyssiere ◽  
...  
Keyword(s):  
Edible Oils ◽  
Pilot Scale ◽  
Scale Production ◽  
Engine Emissions ◽  
Pilot Scale Production

Outdoor pilot-scale production of Botryococcus braunii in panel reactors

2012 ◽  
Vol 24 (6) ◽  
pp. 1353-1360 ◽  
Author(s):  
Jazmin Bazaes ◽  
Claudia Sepulveda ◽  
F. Gabriel Acién ◽  
Juan Morales ◽  
Leonel Gonzales ◽  
...  
Keyword(s):  
Botryococcus Braunii ◽  
Pilot Scale ◽  
Scale Production ◽  
Pilot Scale Production

Pilot-Scale Production and Application of Microparticulated Plant Proteins

2018 ◽  
Vol 08 (01) ◽  
Author(s):  
Milos Beran ◽  
Josef Drahorad ◽  
Ondrej Vltavsky ◽  
Marian Urban ◽  
Iva Laknerova ◽  
...  
Keyword(s):  
Pilot Scale ◽  
Scale Production ◽  
Plant Proteins ◽  
Pilot Scale Production

Development of pilot-scale production of dry bacterial starter cultures for intensive manufacture of high quality meat products

1997 ◽  
Vol 32 (3) ◽  
pp. 191-194
Author(s):  
V.A. Samoylenko ◽  
T.V. Yakshina ◽  
R.R. Oleynikov ◽  
Yu.G. Kostenko ◽  
D.N. Spicyna ◽  
...  
Keyword(s):  
Meat Products ◽  
Pilot Scale ◽  
Starter Cultures ◽  
Scale Production ◽  
High Quality ◽  
Pilot Scale Production

TEACHING PRACTICE: PILOT SCALE PRODUCTION OF A BIODEGRADABLE POLYMER

2018 ◽  
Author(s):  
Miguel Aldas ◽  
Jose Miguel Ferri ◽  
Daniel Garcia-Garcia ◽  
Juan Lopez-Martinez
Keyword(s):  
Biodegradable Polymer ◽  
Teaching Practice ◽  
Pilot Scale ◽  
Scale Production ◽  
Pilot Scale Production
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