The Influences of Supplemental Vegetable Oils on the Growth and β-carotene Accumulation of Oleaginous Yeast-Rhodotorula glutinis

Astaxanthin is a high-value red pigment and antioxidant used by pharmaceutical, cosmetics, and food industries. The astaxanthin produced chemically is costly and is not approved for human consumption due to the presence of by-products. The astaxanthin production by natural microalgae requires large open areas and specialized equipment, the process takes a long time, and results in low titers. Recombinant microbial cell factories can be engineered to produce astaxanthin by fermentation in standard equipment. In this work, an oleaginous yeast Yarrowia lipolytica was engineered to produce astaxanthin at high titers in submerged fermentation. First, a platform strain was created with an optimised pathway towards β-carotene. The platform strain produced 331 ± 66 mg/L of β-carotene in small-scale cultivation, with the cellular content of 2.25% of dry cell weight. Next, the genes encoding β-ketolase and β-hydroxylase of bacterial (Paracoccus sp. and Pantoea ananatis) and algal (Haematococcus pluvialis) origins were introduced into the platform strain in different copy numbers. The resulting strains were screened for astaxanthin production, and the best strain, containing algal β-ketolase and β-hydroxylase, resulted in astaxanthin titer of 44 ± 1 mg/L. The same strain was cultivated in controlled bioreactors, and a titer of 285 ± 19 mg/L of astaxanthin was obtained after seven days of fermentation on complex medium with glucose. Our study shows the potential of Y. lipolytica as the cell factory for astaxanthin production.

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Assessment of β-carotene content, cell physiology and morphology of the yellow yeast Rhodotorula glutinis mutant 400A15 using flow cytometry

Journal of Industrial Microbiology & Biotechnology ◽

10.1007/s10295-013-1278-2 ◽

2013 ◽

Vol 40 (8) ◽

pp. 865-875 ◽

Cited By ~ 11

Author(s):

Raffaela Cutzu ◽

Ana Clemente ◽

Alberto Reis ◽

Beatriz Nobre ◽

Ilaria Mannazzu ◽

...

Keyword(s):

Flow Cytometry ◽

Rhodotorula Glutinis ◽

Cell Physiology ◽

Carotene Content ◽

Β Carotene

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Genome Sequence of the Oleaginous Yeast Rhodotorula glutinis ATCC 204091

Genome Announcements ◽

10.1128/genomea.00046-14 ◽

2014 ◽

Vol 2 (1) ◽

Cited By ~ 18

Author(s):

D. Paul ◽

Z. Magbanua ◽

M. Arick ◽

T. French ◽

S. M. Bridges ◽

...

Keyword(s):

Genome Sequence ◽

Oleaginous Yeast ◽

Rhodotorula Glutinis

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Singlet oxygen autoxidation of vegetable oils: Evidences for lack of synergy between β-carotene and tocopherols

Food Chemistry ◽

10.1016/j.foodchem.2015.02.127 ◽

2015 ◽

Vol 182 ◽

pp. 209-216 ◽

Cited By ~ 7

Author(s):

Bogdan Smyk

Keyword(s):

Singlet Oxygen ◽

Vegetable Oils ◽

Β Carotene

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Effect of Polysorbates on the Growth of Rhodotorula Glutinis in Oil Rich Medium

Environmental and Climate Technologies ◽

10.2478/rtuect-2021-0081 ◽

2021 ◽

Vol 25 (1) ◽

pp. 1075-1085

Author(s):

Svetlana Raita ◽

Kriss Spalvins ◽

Evalds Raits ◽

Inese Silicka ◽

Dagnija Blumberga

Keyword(s):

Oleaginous Yeast ◽

Control Sample ◽

Rhodotorula Glutinis ◽

Inhibitory Effect ◽

Tween 20 ◽

Rich Medium ◽

Yeast Growth ◽

Thermogravimetric Method ◽

Yeast Biomass ◽

Medium Supplementation

Abstract The present study has investigated the effect of oil rich medium supplementation with polysorbates Tween 20, 40 and 80, for the cultivation of red oleaginous yeast Rhodotorula glutinis. R. glutinis has been cultivated in yeast extract peptone glucose modified broth (mYPG) supplemented with 2 % of waste cooking rapeseed oil and three polysorbate types with 0.5 %, 1 %, 2%, 3 %, 4 %, 5 %, 6 %, 7 %, 8 %, 9 % and 10 % concentration each. Yeast biomass was measured by the thermogravimetric method at 105 °C each day during 7-day experiment. The oil rich medium supplementation with Tween 20, 40 and 80 at concentrations ranging from 2 % to 10 % significantly increased the biomass of R. glutinis. All three types of the studied polysorbates with 0.5 % and 1 % concentration, did not affect yeast growth and the dry biomass – results were similar to the control sample without polysorbate addition. Between the three types of polysorbates, Tween 20 was selected as the preferable for R. glutinis cultivation with an optimal concentration of 2 %. Cultivation of R. glutinis in oil rich medium with polysorbates Tween 20, 40 and 80, supplementation up to 10 % concentration did not have had an inhibitory effect on the biomass growth.

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