scholarly journals Age-related changes in the mRNA levels of CYP1A1, CYP2B1/2 and CYP3A1 isoforms in rat small intestine

2011 ◽  
Vol 7 (2) ◽  
pp. 197-207 ◽  
Author(s):  
Artur Pałasz ◽  
Anna Wiaderkiewicz ◽  
Ryszard Wiaderkiewicz ◽  
Piotr Czekaj ◽  
Beata Czajkowska ◽  
...  
Keyword(s):  
Small Intestine ◽  
Mrna Levels ◽  
Rat Small Intestine ◽  
Age Related ◽  
Age Related Changes

Transcriptional regulation of intestinal hydrolase biosynthesis during postnatal development in rats

1994 ◽  
Vol 267 (4) ◽  
pp. G584-G594 ◽  
Author(s):  
S. D. Krasinski ◽  
G. Estrada ◽  
K. Y. Yeh ◽  
M. Yeh ◽  
P. G. Traber ◽  
...  
Keyword(s):  
Small Intestine ◽  
Transcriptional Control ◽  
Mrna Levels ◽  
Rat Small Intestine ◽  
Rnase Protection ◽  
Reciprocal Regulation ◽  
Adult Rat ◽  
Microvillus Membrane ◽  
Specific Activities ◽  
Respective Protein

Lactase-phlorizin hydrolase (LPH) and sucrase-isomaltase (SI) are intestine-specific microvillus membrane hydrolases whose specific activities demonstrate reciprocal regulation during development but whose mechanisms of regulation have not been fully defined. To investigate transcriptional control of these two proteins, the rat LPH and SI genes were cloned, and antisense probes for preprocessed mRNAs (pre-mRNAs) were developed from intron sequence. LPH mRNA, as measured by quantitative ribonuclease (RNase) protection assays, was abundant before weaning and decreased two- to fourfold during weaning, whereas SI mRNA was first detected 14 days after birth and increased rapidly to abundant levels by age 28 days. LPH and SI pre-mRNA levels paralleled those of their respective mRNAs. LPH transcriptional rate declined during weaning, whereas that of SI increased during this time as determined by RNase protection assays of pre-mRNAs and nuclear run-on assays. In the adult rat, LPH mRNA was restricted to the jejunum and proximal ileum, whereas SI mRNA was detected throughout the small intestine, a pattern regulated by transcriptional rate as confirmed by nuclear run-on assays. Lactase and sucrase specific activities correlated well with their respective protein and mRNA concentrations in all experiments. We conclude that gene transcription plays a major role in the developmental and horizontal regulation of LPH and SI biosynthesis and that these two genes are regulated differently in rat small intestine.

scholarly journals Orange Juice and Its Component, Hesperidin, Decrease the Expression of Multidrug Resistance-Associated Protein 2 in Rat Small Intestine and Liver

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Minoru Watanabe ◽  
Naoki Matsumoto ◽  
Yuko Takeba ◽  
Toshio Kumai ◽  
Masami Tanaka ◽  
...  
Keyword(s):  
Small Intestine ◽  
Multidrug Resistance ◽  
Orange Juice ◽  
Tap Water ◽  
Pharmacokinetic Parameters ◽  
Mrna Levels ◽  
Rat Small Intestine ◽  
Sprague Dawley ◽  
Water Ingestion ◽  
Sprague Dawley Rats

We investigated the effects of orange juice (OJ) or hesperidin, a component of OJ, on the pharmacokinetics of pravastatin (PRV) and the expression of both protein and mRNA of multidrug resistance-associated protein 2 (Mrp2) in the rat small intestine and liver. Eight-week-old male Sprague-Dawley rats were used in this study. OJ or a 0.079% hesperidin suspension was administered orally for 2 days. Tap water was given as a control. A single dose of PRV at 100 mg/kg p.o. was administered after 2 days of OJ, hesperidin, or tap water ingestion. The AUC, , andt1/2values of PRV were significantly increased in OJ group. Mrp2 protein and mRNA levels in the small intestine and liver, respectively, were significantly decreased after the ingestion of OJ. The same results were obtained with hesperidin. These results suggest that the changes in PRV pharmacokinetic parameters and the decrease in Mrp2 expression caused by OJ are due to hesperidin in the juice.

Age-dependent alterations in Ca2+ homeostasis: role of TRPV5 and TRPV6

2006 ◽  
Vol 291 (6) ◽  
pp. F1177-F1183 ◽  
Author(s):  
Monique van Abel ◽  
Sylvie Huybers ◽  
Joost G. J. Hoenderop ◽  
Annemiete W. C. M. van der Kemp ◽  
Johannes P. T. M. van Leeuwen ◽  
...  
Keyword(s):  
Vitamin D ◽  
Mrna Levels ◽  
Serum Osteocalcin ◽  
Intestinal Epithelial ◽  
Receptor Mrna ◽  
Age Related ◽  
Age Dependent ◽  
Elder People ◽  
Age Related Changes

Aging is associated with alterations in Ca2+ homeostasis, which predisposes elder people to hyperparathyroidism and osteoporosis. Intestinal Ca2+ absorption decreases with aging and, in particular, active transport of Ca2+ by the duodenum. In addition, there are age-related changes in renal Ca2+ handling. To examine age-related changes in expression of the renal and intestinal epithelial Ca2+ channels, control (TRPV5+/+) and TRPV5 knockout (TRPV5−/−) mice aged 10, 30, and 52 wk were studied. Aging of TRPV5+/+ mice resulted in a tendency toward increased renal Ca2+ excretion and significantly decreased intestinal Ca2+ absorption, which was accompanied by reduced expression of TRPV5 and TRPV6, respectively, despite increased serum 1,25(OH)2D3 levels. Similarly, in TRPV5−/− mice the existing renal Ca2+ loss was more pronounced in elder animals, whereas the compensatory intestinal Ca2+ absorption and TRPV6 expression declined with aging. In both mice strains, aging resulted in a resistance to 1,25(OH)2D3 and diminished renal vitamin D receptor mRNA levels, whereas serum Ca2+ levels remained constant. Furthermore, 52-wk-old TRPV5−/− mice showed severe hyperparathyroidism, whereas PTH levels in elder TRPV5+/+ mice remained normal. In 52-wk-old TRPV5−/− mice, serum osteocalcin levels were increased in accordance with the elevated PTH levels, suggesting an increased bone turnover in these mice. In conclusion, downregulation of TRPV5 and TRPV6 is likely involved in the impaired Ca2+ (re)absorption during aging. Moreover, TRPV5−/− mice likely develop age-related hyperparathyroidism and osteoporotic characteristics before TRPV5+/+ mice, demonstrating the importance of the epithelial Ca2+ channels in Ca2+ homeostasis.

scholarly journals Age‐related increases in microsomal quercetin glucuronidation in rat small intestine.

2009 ◽  
Vol 23 (S1) ◽  
Author(s):  
Bradley W Bolling ◽  
Michael H Court ◽  
Jeffrey B Blumberg ◽  
C‐Y. Oliver Chen
Keyword(s):  
Small Intestine ◽  
Rat Small Intestine ◽  
Age Related

Apolipoprotein A-IV synthesis in rat intestine: regulation by dietary triglyceride

1987 ◽  
Vol 252 (5) ◽  
pp. G662-G666 ◽  
Author(s):  
T. F. Apfelbaum ◽  
N. O. Davidson ◽  
R. M. Glickman
Keyword(s):  
Protein Synthesis ◽  
Small Intestine ◽  
Total Protein ◽  
Mrna Levels ◽  
Rat Small Intestine ◽  
Rat Intestine ◽  
Apolipoprotein A ◽  
Rat Enterocytes

Apolipoprotein A-IV (apoA-IV) synthesis rates were measured in vivo in rat enterocytes by immunoprecipitation after administration of [3H]leucine into in situ loops of jejunum and ileum. Basal apoA-IV synthesis rates (percent total protein synthesis) were significantly higher in jejunal enterocytes (2.05 +/- 0.54%) compared with ileal enterocytes (0.48 +/- 0.32%) from the same fasted animals. After an acute triglyceride bolus, significant and sustained elevations of apoA-IV synthesis rates were seen in both jejunal and ileal enterocytes with maximal effects noted at 4-6 h. Animals fed diets containing 30% wt/wt triglyceride as saturated (SF) or polyunsaturated (UF) fats for 6 wk had similarly increased rates of apoA-IV synthesis in jejunal enterocytes with both SF (3.73 +/- 0.83%) and UF (3.33 +/- 0.64%) but no change in ileal enterocytes. By contrast, animals consuming a fat-free diet for 3 wk had jejunal apoA-IV synthesis rates indistinguishable from basal values (2.40 +/- 0.45%). Translatable intestinal mRNA levels for pre-apoA-IV after triglyceride increased in parallel to synthesis rates with a 50% increase in jejunum and a 350% increase in ileum observed at 4-6 h. These results suggest that apoA-IV synthesis by rat small intestine increases in response to acute and chronic dietary triglyceride, is maintained in the absence of dietary triglyceride, and may be under pretranslational control.

Sign in / Sign up

Export Citation Format

Share Document