Gold nanoparticle-DNA aptamer-assisted delivery of antimicrobial peptide effectively inhibits Acinetobacter baumannii infection in mice

2021 ◽  
Vol 60 (1) ◽  
pp. 128-136
Author(s):  
Jaeyeong Park ◽  
Eunkyoung Shin ◽  
Ji-Hyun Yeom ◽  
Younkyung Choi ◽  
Minju Joo ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Acinetobacter Baumannii ◽  
Gold Nanoparticle ◽  
Dna Aptamer ◽  
Assisted Delivery

scholarly journals Femtomolar and selective dopamine detection by a gold nanoparticle enhanced surface plasmon resonance aptasensor

2018 ◽  
Author(s):  
Yong Cao ◽  
Mark T. McDermott
Keyword(s):  
Surface Plasmon Resonance ◽  
Detection Limit ◽  
Gold Nanoparticle ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Cdna Probe ◽  
Dna Aptamer ◽  
Dopamine Detection ◽  
Calibration Curves ◽  
20 Nm

ABSTRACTUltrasensitive and selective detection and quantification of dopamine (DA) plays a key role in monitoring neurodegenerative diseases. However, the detection limit reported for DA detection is typically in the lower nM range. Pushing the detection limit to pM or lower for this particular target to cover the physiological levels (< 130 pM) is significant. Herein, DA DNA aptamer (DAAPT) gold nanoparticle (AuNP) conjugate is utilized to enhance the surface plasmon resonance (SPR) signal, which enables to detect and quantify DA in the femtomolar (200 fM) to picomolar range. To the best of our knowledge, this is the lowest detection limit achieved for SPR sensing of dopamine. The as-prepared 10 nm DAAPT-AuNP conjugate demonstrates strong binding affinity (Kd = 3.1 ± 1.4 nM) to the complementary DNA (cDNA) probe on gold chip. The cDNA probe is immobilized to the chip via polydopamine surface chemistry, which allows the Michael addition of any primary amine-terminated biomolecules. By adjusting the concentration of the DAAPT-AuNP conjugate, two calibration curves are generated with dynamic ranges from 100 µM to 2 mM, and from 200 fM to 20 nM, respectively. Both calibration curves have negative slopes, showing good agreement to a dose-response curve in an enzyme inhibition assay. In addition, the sensing strategy is evaluated to be specific for DA detection using a series of DA analogs and other metabolites as potential interferences.

Polyvinyl alcohol nanofiber formulation of the designer antimicrobial peptide APO sterilizes Acinetobacter baumannii-infected skin wounds in mice

Amino Acids ◽  
2015 ◽  
Vol 48 (1) ◽  
pp. 203-211 ◽  
Author(s):  
Istvan Sebe ◽  
Eszter Ostorhazi ◽  
Aron Fekete ◽  
Krisztian N. Kovacs ◽  
Romana Zelko ◽  
...  
Keyword(s):  
Polyvinyl Alcohol ◽  
Antimicrobial Peptide ◽  
Acinetobacter Baumannii ◽  
Skin Wounds ◽  
Infected Skin

Simple and Rapid Colorimetric Biosensors Based on DNA Aptamer and Noncrosslinking Gold Nanoparticle Aggregation

ChemBioChem ◽  
2007 ◽  
Vol 8 (7) ◽  
pp. 727-731 ◽  
Author(s):  
Weian Zhao ◽  
William Chiuman ◽  
Michael A. Brook ◽  
Yingfu Li
Keyword(s):  
Gold Nanoparticle ◽  
Dna Aptamer ◽  
Nanoparticle Aggregation ◽  
Gold Nanoparticle Aggregation

Gold nanoparticle-based colorimetric detection of kanamycin using a DNA aptamer

2011 ◽  
Vol 415 (2) ◽  
pp. 175-181 ◽  
Author(s):  
Kyung-Mi Song ◽  
Minseon Cho ◽  
Hunho Jo ◽  
Kyoungin Min ◽  
Sung Ho Jeon ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Colorimetric Detection ◽  
Dna Aptamer

scholarly journals A Systematic Study of the Stability, Safety, and Efficacy of the de novo Designed Antimicrobial Peptide PepD2 and Its Modified Derivatives Against Acinetobacter baumannii

2021 ◽  
Vol 12 ◽  
Author(s):  
Sung-Pang Chen ◽  
Eric H-L Chen ◽  
Sheng-Yung Yang ◽  
Pin-Shin Kuo ◽  
Hau-Ming Jan ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Peptide ◽  
Acinetobacter Baumannii ◽  
De Novo ◽  
Multidrug Resistant ◽  
Hek293 Cells ◽  
Amino Acid Residues ◽  
Bacteria And Fungi ◽  
The Stability

Searching for new antimicrobials is a pressing issue to conquer the emergence of multidrug-resistant (MDR) bacteria and fungi. Antimicrobial peptides (AMPs) usually have antimicrobial mechanisms different from those of traditional antibiotics and bring new hope in the discovery of new antimicrobials. In addition to antimicrobial activity, stability and target selectivity are important concerns to decide whether an antimicrobial peptide can be applied in vivo. Here, we used a simple de novo designed peptide, pepD2, which contains only three kinds of amino acid residues (W, K, L), as an example to evaluate how the residues and modifications affect the antimicrobial activity against Acinetobacter baumannii, stability in plasma, and toxicity to human HEK293 cells. We found that pepI2 with a Leu→Ile substitution can decrease the minimum bactericidal concentrations (MBC) against A. baumannii by one half (4 μg/mL). A D-form peptide, pepdD2, in which the D-enantiomers replaced the L-enantiomers of the Lys(K) and Leu(L) residues, extended the peptide half-life in plasma by more than 12-fold. PepD3 is 3-residue shorter than pepD2. Decreasing peptide length did not affect antimicrobial activity but increased the IC50 to HEK293 cells, thus increased the selectivity index (SI) between A. baumannii and HEK293 cells from 4.7 to 8.5. The chain length increase of the N-terminal acyl group and the Lys→Arg substitution greatly enhanced the hemolytic activity, hence those modifications are not good for clinical application. Unlike colistin, the action mechanism of our peptides relies on negatively charged lipids rather than lipopolysaccharides. Therefore, not only gram-negative bacteria but also gram-positive bacteria can be killed by our peptides.

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