Polyvinyl alcohol nanofiber formulation of the designer antimicrobial peptide APO sterilizes Acinetobacter baumannii-infected skin wounds in mice

Amino Acids ◽  
2015 ◽  
Vol 48 (1) ◽  
pp. 203-211 ◽  
Author(s):  
Istvan Sebe ◽  
Eszter Ostorhazi ◽  
Aron Fekete ◽  
Krisztian N. Kovacs ◽  
Romana Zelko ◽  
...  
Keyword(s):  
Polyvinyl Alcohol ◽  
Antimicrobial Peptide ◽  
Acinetobacter Baumannii ◽  
Skin Wounds ◽  
Infected Skin

Gold nanoparticle-DNA aptamer-assisted delivery of antimicrobial peptide effectively inhibits Acinetobacter baumannii infection in mice

2021 ◽  
Vol 60 (1) ◽  
pp. 128-136
Author(s):  
Jaeyeong Park ◽  
Eunkyoung Shin ◽  
Ji-Hyun Yeom ◽  
Younkyung Choi ◽  
Minju Joo ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Acinetobacter Baumannii ◽  
Gold Nanoparticle ◽  
Dna Aptamer ◽  
Assisted Delivery

An injectable adhesive antibacterial hydrogel wound dressing for infected skin wounds

2021 ◽  
pp. 112584
Author(s):  
Wendi Ma ◽  
Wenying Dong ◽  
Shanshan Zhao ◽  
Tianhui Du ◽  
Yilong Wang ◽  
...  
Keyword(s):  
Wound Dressing ◽  
Skin Wounds ◽  
Infected Skin

scholarly journals Topical Application of Cinnamaldehyde Promotes Faster Healing of Skin Wounds Infected with Pseudomonas aeruginosa

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1627 ◽  
Author(s):  
Thiago A.F. Ferro ◽  
Eliene B. Souza ◽  
Mariela A.M. Suarez ◽  
João F.S. Rodrigues ◽  
Domingos M.S. Pereira ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Topical Application ◽  
Transient Receptor Potential ◽  
Bacterial Load ◽  
Unmet Need ◽  
Antimicrobial Properties ◽  
Mouse Skin ◽  
Interleukin 17 ◽  
Skin Wounds ◽  
Infected Skin

Wound healing can be delayed following colonization and infection with the common bacterium Pseudomonas aeruginosa. While multiple therapies are used for their treatment, these are ineffective, expensive, and labour-intensive. Thus, there is an enormous unmet need for the treatment of infected wounds. Cinnamaldehyde, the major component of cinnamon oil, is well known for its antimicrobial properties. Herein, we investigated the effects of sub-inhibitory concentrations of cinnamaldehyde in the virulence of P. aeruginosa. We also assessed its healing potential in P. aeruginosa-infected mouse skin wounds and the mechanisms involved in this response. Sub-inhibitory concentrations of cinnamaldehyde reduced P. aeruginosa metabolic rate and its ability to form biofilm and to cause haemolysis. Daily topical application of cinnamaldehyde on P. aeruginosa-infected skin wounds reduced tissue bacterial load and promoted faster healing. Lower interleukin-17 (IL-17), vascular endothelial growth factor (VEGF) and nitric oxide levels were detected in cinnamaldehyde-treated wound samples. Blockage of transient receptor potential ankyrin 1, the pharmacological target of cinnamaldehyde, abrogated its healing activity and partially reversed the inhibitory actions of this compound on VEGF and IL-17 generation. We suggest that topical application of sub-inhibitory concentrations of cinnamaldehyde may represent an interesting approach to improve the healing of P. aeruginosa-infected skin wounds.

scholarly journals The bactericidal effect of lysostaphin coupled with liposomal vancomycin as a dual combating system applied directly on methicillin-resistant Staphylococcus aureus infected skin wounds in mice 

2019 ◽  
Vol Volume 14 ◽  
pp. 5943-5955 ◽  
Author(s):  
Fahimeh Hajiahmadi ◽  
Mohammad Yousef Alikhani ◽  
Hanifeh Shariatifar ◽  
Mohammad Reza Arabestani ◽  
Davoud Ahmadvand
Keyword(s):  
Staphylococcus Aureus ◽  
Bactericidal Effect ◽  
Skin Wounds ◽  
Methicillin Resistant ◽  
Infected Skin

In-depth characterization of antibacterial activity of melittin against Staphylococcus aureus and use in a model of non-surgical MRSA-infected skin wounds

2021 ◽  
Vol 156 ◽  
pp. 105592
Author(s):  
William Gustavo Lima ◽  
Júlio César Moreira de Brito ◽  
Valbert Nascimento Cardoso ◽  
Simone Odília Antunes Fernandes
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Skin Wounds ◽  
Infected Skin

scholarly journals A Systematic Study of the Stability, Safety, and Efficacy of the de novo Designed Antimicrobial Peptide PepD2 and Its Modified Derivatives Against Acinetobacter baumannii

2021 ◽  
Vol 12 ◽  
Author(s):  
Sung-Pang Chen ◽  
Eric H-L Chen ◽  
Sheng-Yung Yang ◽  
Pin-Shin Kuo ◽  
Hau-Ming Jan ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Peptide ◽  
Acinetobacter Baumannii ◽  
De Novo ◽  
Multidrug Resistant ◽  
Hek293 Cells ◽  
Amino Acid Residues ◽  
Bacteria And Fungi ◽  
The Stability

Searching for new antimicrobials is a pressing issue to conquer the emergence of multidrug-resistant (MDR) bacteria and fungi. Antimicrobial peptides (AMPs) usually have antimicrobial mechanisms different from those of traditional antibiotics and bring new hope in the discovery of new antimicrobials. In addition to antimicrobial activity, stability and target selectivity are important concerns to decide whether an antimicrobial peptide can be applied in vivo. Here, we used a simple de novo designed peptide, pepD2, which contains only three kinds of amino acid residues (W, K, L), as an example to evaluate how the residues and modifications affect the antimicrobial activity against Acinetobacter baumannii, stability in plasma, and toxicity to human HEK293 cells. We found that pepI2 with a Leu→Ile substitution can decrease the minimum bactericidal concentrations (MBC) against A. baumannii by one half (4 μg/mL). A D-form peptide, pepdD2, in which the D-enantiomers replaced the L-enantiomers of the Lys(K) and Leu(L) residues, extended the peptide half-life in plasma by more than 12-fold. PepD3 is 3-residue shorter than pepD2. Decreasing peptide length did not affect antimicrobial activity but increased the IC50 to HEK293 cells, thus increased the selectivity index (SI) between A. baumannii and HEK293 cells from 4.7 to 8.5. The chain length increase of the N-terminal acyl group and the Lys→Arg substitution greatly enhanced the hemolytic activity, hence those modifications are not good for clinical application. Unlike colistin, the action mechanism of our peptides relies on negatively charged lipids rather than lipopolysaccharides. Therefore, not only gram-negative bacteria but also gram-positive bacteria can be killed by our peptides.

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