Ethyl Methanesulfonate Mutagenesis and In Vitro Polyethylene Glycol Selection for Drought Tolerance in Sugarcane (Saccharum spp.)

Sugar Tech ◽  
2017 ◽  
Vol 20 (1) ◽  
pp. 50-59 ◽  
Author(s):  
M. Masoabi ◽  
J. Lloyd ◽  
J. Kossmann ◽  
C. van der Vyver
Keyword(s):  
Polyethylene Glycol ◽  
Drought Tolerance ◽  
Ethyl Methanesulfonate ◽  
Saccharum Spp ◽  
Selection For ◽  
Ethyl Methanesulfonate Mutagenesis

scholarly journals Recurrent Selection for Improved Germination under Water Stress in Russian Dandelion

2017 ◽  
Vol 142 (2) ◽  
pp. 85-91 ◽  
Author(s):  
Katrina J.M. Hodgson-Kratky ◽  
Olivier M. Stoffyn ◽  
David J. Wolyn
Keyword(s):  
Polyethylene Glycol ◽  
Water Stress ◽  
Recurrent Selection ◽  
Field Experiments ◽  
Phenotypic Selection ◽  
Bare Soil ◽  
Promising Candidate ◽  
Breeding Programs ◽  
Selection For

Russian dandelion [Taraxacum kok-saghyz (TKS)] is a promising candidate for introducing natural rubber production into North America. Seeds normally germinate in a humid microenvironment, such as the thatch layer of a lawn or under a canopy of grass; however, 5% to 15% establishment is often observed on bare soil, presumably due to water stress. Phenotypic selection and half-sib family recurrent selection were conducted for three cycles to improve germination in vitro, under low osmotic potential (Ψs), using a polyethylene glycol (PEG) solution. Populations were then tested for establishment on bare soil in the greenhouse and field. Germination under water stress in vitro increased from 5.8% for the cycle 0 (C0) population to 40.8% and 47.8% for the C3-phenotypic and C3-half-sib family populations, respectively. Soil establishment in the greenhouse and field was improved up to two- and 4-fold, respectively, compared with the C0, in two of four greenhouse experiments and three of eight field experiments. Overall, recurrent selection for germination under water stress in vitro has potential to improve establishment in the field and can be incorporated into current breeding programs to support the overall goal of creating cultivars with high-rubber yield.

scholarly journals The Growth Response of Several Potato Genotypes (Solanum tuberosum L.) to Induced Water Stress Using Sorbitol and Polyethylene Glycol

2016 ◽  
Vol 8 (4) ◽  
pp. 511-519 ◽  
Author(s):  
Mihaela A. CIOLOCA ◽  
Andreea M. TICAN ◽  
Maria IANOŞI ◽  
Carmen L. BĂDĂRĂU
Keyword(s):  
Polyethylene Glycol ◽  
Water Stress ◽  
Drought Tolerance ◽  
Growth Response ◽  
Culture Medium ◽  
Growth Parameters ◽  
The Other ◽  
Addition Lines ◽  
In Vitro Response

The current paper aimed to study the in vitro response of potato genotypes to water stress induced by adding sorbitol and polyethylene glycol in the culture medium. The biological material analysed in the experiment was represented by a Romanian line ‘LP 11-1525/1’ and two isogenic lines ‘LI 101’ and ‘LI 102’. For cultures initiation, the line ‘LP 11-1525/1’ was started from meristems and for the other two genotypes true potato seeds were used. The studied potato genotypes behaved differently depending on the analysed parameters and on the treatment applied for drought tolerance. It was noted that the line ‘LP 11-1525/1’ achieved good results for most of the growth parameters studied, and also the lines derived from true potato seeds behaved well, in some cases even exceeding the line derived from meristems. Of the lines derived from true potato seeds, the best performance was noted for line ‘LI 101-6’ in all the analysed parameters, both on sorbitol and PEG medium. In addition, lines ‘LI 101-7’ and ‘LI 102-4’ achieved good results on both variants of medium used to mediate water stress. Therefore, establishing drought tolerance individuals within populations derived from true potato seeds using sorbitol and polyethylene glycol might be applied.

scholarly journals Selection and regeneration of purple sweet potato calli against drought stress simulated by polyethylene glycol

F1000Research ◽  
2019 ◽  
Vol 8 ◽  
pp. 10
Author(s):  
Widi Sunaryo ◽  
Darnaningsih Darnaningsih ◽  
Nurhasanah Nurhasanah
Keyword(s):  
Polyethylene Glycol ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Sweet Potato ◽  
Crop Production ◽  
Callus Formation ◽  
Ms Medium ◽  
New Variant ◽  
Purple Sweet Potato

Background: Water shortage due to natural and/or technical drought stress, widespread throughout Sumatra, Java, Sulawesi and Kalimantan islands, significantly reduces crop production. The development of varieties tolerant to drought stress is important since it is more effective rather than improving irrigation infrastructure to increase the sweet potato productivity. Methods: Selection and regeneration experiments assessing purple sweet potato callus tolerance of drought stress, simulated by polyethylene glycol (PEG), were conducted to generate new variant plants tolerant of drought stress. Sterile explants (leaf and petiole) generated from previous in vitro culture were inoculated to the Murishage and Skoog (MS) medium containing plant growth regulator combination as treatments to induce calli. The calli were then transferred to half-MS medium containing 0, 5, 10, 15 and 20% PEG as selection agent for drought tolerance. The surviving calli were regenerated in the MS medium containing 0, 0.5, 1 or 1.5 mg l-1 6-benzylaminopurine (BAP). The callus formation, growth and survivability during in vitro culture were measured. Results: Calli were successfully formed in almost all media containing 2,4-Dichlorophenoxyacetic acid (2,4-D ) with the concentration of 1, 2, 3 and 4 mg l-1 and BAP (concentration: 0.5 and 1 mg l-1), but the medium of MS + 2 mg l-1 2,4-D + 0.5 mg l-1 BAP resulted in the highest number of induced calli per treatment (mean=11.36), with the percentage of responsive explants standing at around 96%. The higher the concentration of PEG, the lower the number of surviving calli. At 20% PEG, only 54.42% calli survived. There were five plants successfully regenerated from the survived calli at 20% PEG, using MS medium containing 1.5 mg l-1 BAP. Conclusions: The experiment has successfully produced putative drought-tolerant plants by callus screening using PEG as drought-tolerance-selecting agent in purple sweet potato.

Somaclonal Variation in Rice ̵2 Drought Tolerance and Other Agronomic Characters

1995 ◽  
Vol 43 (2) ◽  
pp. 201 ◽  
Author(s):  
SW Adkins ◽  
R Kunanuvatchaidach ◽  
ID Godwin
Keyword(s):  
Polyethylene Glycol ◽  
Drought Tolerance ◽  
Somaclonal Variation ◽  
Environmental Conditions ◽  
In Vitro Selection ◽  
Dry Weight ◽  
Rice Seed ◽  
Agronomic Characters ◽  
Selection Step

The ability to withstand drought was studied in R2 families raised by self-pollination from 149 R1 plants regenerated from callus of mature rice seed (cvs FR13A, Kurkaruppan, Khao Dawk Mali 105, Nam Sagui 19, and Calrose 76). Compared to parental controls, significant improvement in drought tolerance as assessed by dry weight production, was found in 6% of the cv. FR13A families, but not in any of Kurkaruppan or Khao Dawk Mali 105 families tested in a controlled-temperature glasshouse. When a similar study was undertaken using an in vitro selection step (using polyethylene glycol), significant improvement in drought tolerance was found in 6% of Khao Dawk Mali 105 families, but not in any of the Nam Sagui 19 families. No improvements were noted in this latter study when in vitro selection was not used. Variation in other agronomically important characters was observed in the R2 families grown under similar, but well-watered, environmental conditions.

scholarly journals Drought tolerance selection of GT1 rubber seedlings with the addition of polyethylene glycol (PEG) 6000

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
SYARIFAH AINI PASARIBU ◽  
Mohammad Basyuni ◽  
EDISON PURBA ◽  
YAYA HASANAH
Keyword(s):  
Polyethylene Glycol ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Morphological Characters ◽  
Sensitivity Index ◽  
Shoot Height ◽  
Peg 6000 ◽  
Rate Of Increase ◽  
Osmotic Solution

Abstract. Pasaribu SA, Basyuni M, Purba E, Hasanah Y. 2021. Drought tolerance selection of GT1 rubber seedlings with the addition of polyethylene glycol (PEG) 6000. Biodiversitas 22: 394-400. Tolerance of the rootstock of rubber to drought stress is not optimal. The root system can therefore be used as an indicator of drought tolerance to inhibit the growth of the plant. A good root system of rubber rootstocks is thought to help the plants acquire greater resistance to drought stress. Polyethylene glycol 6000 osmotic solution controls the water potential in the growing medium. This study aimed to analyze the effect of PEG 6000 osmotic solution (0%; 7.5%; and 15% concentration) on rubber seedlings leading to the development of morphological characters and sensitivity index. GT1 (Gondang Tapen 1) rubber seeds to be used as planting material were collected from the seed source garden of PT Socfin Indonesia. The study was carried out from January to February 2019, using a non-factorial randomized block design. The morphological characters observed were tapped root length, shoot height, the increased rate of taproot length, shoot height increase rate, ratio of taproot length, and shoot height. Data were analyzed using analysis of variance, discriminant, and drought stress sensitivity index. The results showed that the addition of PEG 6000 in planting media in vitro significantly influenced the character of the rate of increase of taproot length. The sensitivity index of taproot increase rate and the ratio of taproot length and shoot height was moderate at 7.5% levely contrast, the addition of PEG 6000 in planting media in vitro did not significantly impact it. The present study suggested that the rate of increase of taproot length was a distinguishing character showing the initial tolerance level of the GT1 seedlings to drought.

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