Effects of Brassinolide with Naphthalene Acetic Acid on the Formation of Adventitious Roots, Trichome-Like Roots and Calli from Cultured Tobacco Leaf Segments, and the Expression Patterns of CNT103

2009 ◽  
Vol 52 (6) ◽  
pp. 511-517 ◽  
Author(s):  
Min-Suk Kwak ◽  
In-Hyeon Kim ◽  
Seong-Ki Kim ◽  
Tae-Jin Han
Keyword(s):  
Acetic Acid ◽  
Adventitious Roots ◽  
Expression Patterns ◽  
Naphthalene Acetic Acid ◽  
Tobacco Leaf ◽  
Leaf Segments

scholarly journals Vegetative propagation of native fruit species of páramo Thibaudia floribunda and Cavendishia bracteata by cuttings

2017 ◽  
Vol 35 (1) ◽  
pp. 12-22
Author(s):  
Lina María López C. ◽  
Mariam Vásquez M. ◽  
Héctor Lancheros R. ◽  
Stanislav Magnitskiy
Keyword(s):  
Acetic Acid ◽  
Adventitious Roots ◽  
Naphthalene Acetic Acid ◽  
Fruit Species ◽  
Wild Fruit ◽  
The Andes ◽  
Shoot Production ◽  
Parenchyma Cells ◽  
Rooting Of Cuttings ◽  
Andes Region

Cavendishia bracteata and Thibaudia floribunda (Ericaceae) are wild fruit shrubs native to the Andes region. This study aimed to characterize the rooting potential of basal and apical cuttings using naphthalene acetic acid (NAA) in different concentrations (0, 200, 500, or 1,000 mg L-1). In C. bracteata, the cutting position on the branch affected its sprouting and rooting, with apical cuttings presenting the best performance with auxin application. The rooting of cuttings was more successful in C. bracteata than in T. floribunda. NAA applications between 200 to 500 mg L-1 were more suitable for the propagation of C. bracteata due to the higher percentages of rooting and shoot production. For propagation of T. floribunda, the use of apical cuttings without the application of auxins is recommended. Adventitious roots in cuttings of both species were originated from parenchyma cells of the stem.

scholarly journals Determining the Rooting Ability of Hardwood Cuttings of Blueberry (Vaccinium corymbosum L.) cv. ‘Bluecrop’ Stored in Refrigerating Conditions

2020 ◽  
Vol 8 (3) ◽  
pp. 81
Author(s):  
Sabri Braha ◽  
Petrit Rama ◽  
Agim Zajmi
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Adventitious Roots ◽  
Vaccinium Corymbosum ◽  
Mother Plant ◽  
Winter Period ◽  
Naphthalene Acetic Acid ◽  
Intact Control ◽  
Rooting Ability ◽  
Time Periods

This experiment has been carried out in order to determine the rooting ability of hardwood cuttings of the blueberry cultivar ‘Bluecrop’ collected during the deep dormant winter period in the last part of January. In order to induce the adventitious roots formation, the cuttings were stimulated using Indol Butyric Acid (IBA) and Naphthalene Acetic Acid (NAA) in various concentrations (1500, 3000, 4500 ppm), whilst one row in each box remained intact (control).  The collection of cuttings off the mother plant at the beginning of January enable the nutrients and other matter that help the process of rooting not to move from the base of cutting towards the top and consequently they remain at the base of cutting which results with quite a high rooting percentage (up to 72.5%) compared to the cuttings collected in February with rooting percentage of 67.5% and in March (52%). In both types of substrate, turf and turf-perlite 2:1 and in different time periods when cuttings have been collected, IBA proved to be better in inducing adventitious roots compared to NAA.

scholarly journals Auxin Type, Auxin Concentration, and Air and Substrate Temperature Difference Play Key Roles in the Rooting of Juvenile Hardwood Pecan Cuttings

2015 ◽  
Vol 25 (2) ◽  
pp. 209-213 ◽  
Author(s):  
Ji-Yu Zhang ◽  
Zhong-Ren Guo ◽  
Rui Zhang ◽  
Yong-Rong Li ◽  
Lin Cao ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Substrate Temperature ◽  
Adventitious Roots ◽  
Carya Illinoinensis ◽  
Naphthalene Acetic Acid ◽  
Ambient Temperatures ◽  
Auxin Concentration ◽  
Rooting Of Cuttings ◽  
Bottom Heat ◽  
Indole 3 Acetic Acid

This study examined the ability to vegetatively propagate 1-year-old pecan (Carya illinoinensis) through the rooting of hardwood cuttings. Cuttings were treated with varying concentrations of different auxins and different combinations of media and ambient temperatures. Under different temperature conditions, all auxin treatments induced the rooting of cuttings but did not promote sprouting. The effectiveness of the induction of adventitious roots was as follows: 1-naphthalene acetic acid (NAA) > indole 3-butyric acid > indole 3-acetic acid. The base of the parent shoot treated by NAA at a concentration of 0.09%, planted in substrate with bottom heat was the most effective, with 82% rooting, 8.3 roots/cutting and root lengths of 7.3 cm. These findings suggested that auxin and substrate/air temperature differences are both indispensable in the process of adventitious roots formation in pecan. This study revealed that the propagation of hardwood cuttings derived from branches of 1-year-old pecan is possible.

scholarly journals Somatic embryogenesis in the leaf tissue of Hedyotis diffusa Willd.

2018 ◽  
Vol 1 (6) ◽  
pp. 76-85
Author(s):  
Anh Thi Kim Nguyen ◽  
Tham Thi Thu Hoang ◽  
Hoang Ngo Phan
Keyword(s):  
Acetic Acid ◽  
Somatic Embryogenesis ◽  
Somatic Embryo ◽  
Morphological Changes ◽  
Leaf Tissue ◽  
Naphthalene Acetic Acid ◽  
Mature Embryos ◽  
Embryo Formation ◽  
Hedyotis Diffusa ◽  
Leaf Segments

Hedyotis diffusa is a valuable medicinal herb belong to the Rubiaceae family. It is widely used in the treatment of various types of cancer and other diseases related to leukemia. Besides, the plant usually contains triterpenoids (oleanolic acid, ursolic acid) and flavonoids which have a range of pharmacological activities of antiinflammatory, antibacterial, hypoglycemic, antifree radicals, reducing blood lipids and anticancer. Leaf segments of 3 weeks old H. diffusa were cultured on half strength Murashige and Skoog (½MS) medium supplemented with different concentrations of indole acetic acid (IAA; 0.1, 0.2 and 0.4 mg/L) or α-naphthalene acetic acid (NAA; 0.1, 0.2 and 0.4 mg/L) and benzyladenine (BA) at a concentration of 1mg/L. In this study, the highest percentage of somatic embryogenesis was obtained using 0.1 mg/L IAA and 1 mg/L BA, and 0.2 mg/L IAA and 1 mg/L BA. The somatic embryos develop through a series of morphological stages: globular type, heart, torpedo and mature embryos. Besides, the highest number of shoots per explant was achieved in the same media. The middle and the distal end segments were found the most suitable for somatic embryogenesis. Morphological changes and the role of endogenous hormones in somatic embryo formation were analyzed. The position of the leaf segments of the same leaf, respiration rate, and endogenous hormone and somatic embryo formation were also discussed.

scholarly journals Tissue Culture and Plant Regeneration of Cowpea (Vigna unguiculata)

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 627f-627
Author(s):  
Lurline Marsh
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Plant Regeneration ◽  
Vigna Unguiculata ◽  
Nutrient Medium ◽  
Cotyledonary Node ◽  
Naphthalene Acetic Acid ◽  
Leaf Segments ◽  
Dichlorophenoxy Acetic Acid ◽  
Cotyledonary Node Explants

Explants (cotyledon, cotyledonary node, second node, hypocotyl, epicotyl, and leaf) of cowpea (Vigna unguiculata) genotypes MN13 and Pinkeye Purple Hull were cultured on Murashige and Skoog basal nutrient medium. The medium was supplemented with 1 mg·L–1 benzyladenine (BA) or 1 mg·L–1 benzyladenine plus naphthalene acetic acid (BA + NAA) or 2 mg·L–1 2,4-dichlorophenoxy acetic acid (2,4-D). Cultures were maintained at 22°C for 1 month, after which they were transferred to 1 mg·L–1 BA + NAA. Cotyledons, hypocotyl, epicotyl, and leaf segments produced only calli after subculturing in BA + NAA. The second node and cotyledonary node explants cultured on the BA or BA + NAA followed by subculture on BA + NAA produced calli, shoots, and roots. The plants were then transplanted to promix but later died.

FTIR, GC-MS and HPLC analysis of Baliospermum montanum (Willd.) Muell. Arg.

2020 ◽  
Vol 11 (4) ◽  
pp. 5059-5066
Author(s):  
Sushma B K ◽  
Raveesha H R
Keyword(s):  
Acetic Acid ◽  
Quantitative Estimation ◽  
Chemical Constituents ◽  
Standard Procedure ◽  
Naphthalene Acetic Acid ◽  
Root Extract ◽  
Pentanoic Acid ◽  
Trimethylsilyl Ester ◽  
Isolation And Characterization ◽  
Ms Analysis

The present work is aimed to determine the chemical constituents in Baliospermum montanum methanolic extracts. An in vitro regenerated procedure was developed for the induction of callus from stem explant cultured on Murashige and Skoog (MS) medium fortified with various concentration and permutations of 2, 4-dichloro phenoxy acetic acid, 1-naphthalene acetic acid, 6-benzyl amino purine and gibberellic acid. FTIR & GC-MS analysis was done according to standard procedure. The quantitative estimation of β-sitosterol was done by HPLC method. Maximum fresh and dry weight of callus was estimated in the combination of GA3 (0.5 mg/L) + NAA (2 mg/L) compared to other concentration. The FTIR analysis showed various functional compounds with different characteristic peak values in the extracts. Major bioactive constituents were recognized in the GC-MS analysis. Root extract revealed the existence of 1-hexadecanol, pentanoic acid, 2-(aminooxy)- and 1-hexacosanol. Leaf extract showed the presence of propanoic acid, 2-oxo-, trimethylsilyl ester, 9,12-octadecadienoic acid (z,z)-, trimethylsilyl ester, docosane, 1,22-dibromo- and pentatriacontane. Stem and stem derived callus exhibit the presence of 1,6,3,4-dihydro-2-deoxy-beta-d-lyxo-hexopyranose, n-hexadecanoic acid and pentanoic acid, 2-(aminooxy). The methanolic extract of leaf exhibited 0.2149 % of β-sitosterol content. There were no peaks observed in the root, stem and stem derived callus. Further studies are necessary for the isolation and characterization of bioactive compounds from B. montanum.

PENGARUH KOMBINASI INTENSITAS NAUNGAN DENGAN ZAT PENGATUR TUMBUH INDOLE BUTIRIC ACID (IBA), NAPHTHALENE ACETIC ACID (NAA), DAN VITAMIN B1 DALAM AKLIMATISASI PERTUMBUHAN BIBIT GAHARU (Aquilaria beccariana)

2013 ◽  
Vol 14 (3) ◽  
Author(s):  
Daru Mulyono
Keyword(s):  
Acetic Acid ◽  
Factorial Design ◽  
Vitamin B1 ◽  
Naphthalene Acetic Acid ◽  
Randomized Design ◽  
Optimal Formula

The objective of this research is to know the optimal formula of Indole Butiric Acid (IBA), Naphthalene Acetic Acid (NAA), Vitamine B1 and the combination with shading intensities to the acclimatization of Gaharu stump (Aquilaria beccariana). This research used Factorial Design with basic analysis of Complete Randomized Design in order to know theeffect of treatment. The research was carried out in Agroindustry and Biotechnology Laboratory, Ciampea, Bogor, from July to September 2007. The results of the research showed that after 8 weeks of treatment: (a). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 10 mg/l + Vitamine B1 1 mg/l was the best formula for increasingheight of Gaharu stump 4.660 cm. (b). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 30 mg/l + Vitamine B1 1 mg/l was the best formula for increasing sum of Gaharu leaf stump 12.337 leafs, (c). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 40 mg/l + Vitamine B1 1 mg/l was the best formula for increasing sumof Gaharu root stump 3.783 roots, and (d). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 40 mg/l + Vitamine B1 1 mg/l was the best formula for increasing length of Gaharu root stump 3.686 cm.

scholarly journals Influence of growth regulators and explants on shoot regeneration in carnation

2009 ◽  
Vol 36 (No. 4) ◽  
pp. 140-146 ◽  
Author(s):  
J.K. Kanwar ◽  
S. Kumar
Keyword(s):  
Acetic Acid ◽  
Shoot Regeneration ◽  
Growth Regulators ◽  
Dianthus Caryophyllus ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Shoot Bud ◽  
Dichlorophenoxy Acetic Acid ◽  
Number Of Shoots

The influence of growth regulators, explants and their interactions on in vitro shoot bud formation from callus was studied in <I>Dianthus caryophyllus</I> L. The leaf and internode explants were cultured on Murashige and Skoog (MS) medium containing different concentrations of growth regulators. The highest callus induction was observed with 2 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D) and 1 mg/l benzyl adenine (BA). Out of twenty seven shoot regeneration media tested, only 2 mg/l thidiazuron (TDZ) and zeatin alone or in combination with naphthalene acetic acid (NAA) and/or indole acetic acid (IAA) could differentiate calli. The highest average number of shoots was observed with 2 mg/l TDZ and 1 mg/l IAA. Significant differences were observed in calli producing shoots and number of shoots per callus in the explants of leaf and internode. The shoots were elongated and multiplied on MS medium supplemented with 1 mg/l BA and solidified with 1% agar. The shoots were rooted and hardened with 76% survival success in pots after six weeks of transfer to the pots.

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