Synergistic Effect of High Hydrostatic Pressure (HHP) and Marination Treatment on the Inactivation of Hepatitis A Virus in Mussels (Mytilus galloprovincialis)

ABSTRACT Previous results demonstrated that hepatitis A virus (HAV) could be inactivated by high hydrostatic pressure (HHP) (D. H. Kingsley, D. Hoover, E. Papafragkou, and G. P. Richards, J. Food Prot. 65:1605-1609, 2002); however, direct evaluation of HAV inactivation within contaminated oysters was not performed. In this study, we report confirmation that HAV within contaminated shellfish is inactivated by HHP. Shellfish were initially contaminated with HAV by using a flowthrough system. PFU reductions of >1, >2, and >3 log10 were observed for 1-min treatments at 350, 375, and 400 megapascals, respectively, within a temperature range of 8.7 to 10.3Ã¯Â¿Â½C. Bioconcentration of nearly 6 log10 PFU of HAV per oyster was achieved under simulated natural conditions. These results suggest that HHP treatment of raw shellfish will be a viable strategy for the reduction of infectious HAV.

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Inactivation of Hepatitis A Virus and a Calicivirus by High Hydrostatic Pressure†

Journal of Food Protection ◽

10.4315/0362-028x-65.10.1605 ◽

2002 ◽

Vol 65 (10) ◽

pp. 1605-1609 ◽

Cited By ~ 164

Author(s):

DAVID H. KINGSLEY ◽

DALLAS G. HOOVER ◽

EFI PAPAFRAGKOU ◽

GARY P. RICHARDS

Keyword(s):

Tissue Culture ◽

Hydrostatic Pressure ◽

High Hydrostatic Pressure ◽

Hepatitis A ◽

Hepatitis A Virus ◽

Virus Inactivation ◽

Dependent Manner ◽

Norwalk Virus ◽

Rnase Protection ◽

Infectious Dose

Potential application of high hydrostatic pressure processing (HPP) as a method for virus inactivation was evaluated. A 7-log10 PFU/ml hepatitis A virus (HAV) stock, in tissue culture medium, was reduced to nondetectable levels after exposure to more than 450 MPa of pressure for 5 min. Titers of HAV were reduced in a time- and pressure-dependent manner between 300 and 450 MPa. In contrast, poliovirus titer was unaffected by a 5-min treatment at 600 MPa. Dilution of HAV in seawater increased the pressure resistance of HAV, suggesting a protective effect of salts on virus inactivation. RNase protection experiments indicated that viral capsids may remain intact during pressure treatment, suggesting that inactivation was due to subtle alterations of viral capsid proteins. A 7-log10 tissue culture infectious dose for 50% of the cultures per ml of feline calicivirus, a Norwalk virus surrogate, was completely inactivated after 5-min treatments with 275 MPa or more. These data show that HAV and a Norwalk virus surrogate can be inactivated by HPP and suggest that HPP may be capable of rendering potentially contaminated raw shellfish free of infectious viruses.

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Molecular Basis of the Behavior of Hepatitis A Virus Exposed to High Hydrostatic Pressure

Applied and Environmental Microbiology ◽

10.1128/aem.01693-14 ◽

2014 ◽

Vol 80 (20) ◽

pp. 6499-6505 ◽

Cited By ~ 8

Author(s):

Lucía D'Andrea ◽

Francisco J. Pérez-Rodríguez ◽

M. Isabel Costafreda ◽

Nerea Beguiristain ◽

Cristina Fuentes ◽

...

Keyword(s):

Hydrostatic Pressure ◽

High Hydrostatic Pressure ◽

Molecular Basis ◽

Hepatitis A ◽

Hepatitis A Virus ◽

Fold Axis ◽

Food Borne ◽

Key Factor ◽

Virucidal Effect ◽

Ph Dependent

ABSTRACTFood-borne hepatitis A outbreaks may be prevented by subjecting foods at risk of virus contamination to moderate treatments of high hydrostatic pressure (HHP). A pretreatment promoting hepatitis A virus (HAV) capsid-folding changes enhances the virucidal effect of HHP, indicating that its efficacy depends on capsid conformation. HAV populations enriched in immature capsids (125S provirions) are more resistant to HHP, suggesting that mature capsids (150S virions) are more susceptible to this treatment. In addition, the monoclonal antibody (MAb) K24F2 epitope contained in the immunodominant site is a key factor for the resistance to HHP. Changes in capsid folding inducing a loss of recognition by MAb K24F2 render more susceptible conformations independently of the origin of such changes. Accordingly, codon usage-associated folding changes and changes stimulated by pH-dependent breathings, provided they confer a loss of recognition by MAb K24F2, induce a higher susceptibility to HHP. In conclusion, the resistance of HAV to HHP treatments may be explained by a low proportion of 150S particles combined with a good accessibility of the epitope contained in the immunodominant site close to the 5-fold axis.

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Inactivation of hepatitis A virus by heat and high hydrostatic pressure: variation among laboratory strains

Vox Sanguinis ◽

10.1111/j.1423-0410.2008.01113.x ◽

2009 ◽

Vol 96 (1) ◽

pp. 14-19 ◽

Cited By ~ 22

Author(s):

N. Shimasaki ◽

T. Kiyohara ◽

A. Totsuka ◽

K. Nojima ◽

Y. Okada ◽

...

Keyword(s):

Hydrostatic Pressure ◽

High Hydrostatic Pressure ◽

Hepatitis A ◽

Hepatitis A Virus ◽

Pressure Variation

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Effectiveness of depuration for hepatitis A virus removal from mussels (Mytilus galloprovincialis)

International Journal of Food Microbiology ◽

10.1016/j.ijfoodmicro.2014.04.001 ◽

2014 ◽

Vol 180 ◽

pp. 24-29 ◽

Cited By ~ 13

Author(s):

David Polo ◽

Cristina Álvarez ◽

Ángeles Longa ◽

Jesús L. Romalde

Keyword(s):

Hepatitis A ◽

Mytilus Galloprovincialis ◽

Hepatitis A Virus ◽

Virus Removal

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Synergistic effect of carbon dioxide atmospheres and high hydrostatic pressure to reduce spoilage bacteria on poultry sausages

LWT ◽

10.1016/j.lwt.2014.03.041 ◽

2014 ◽

Vol 58 (2) ◽

pp. 404-411 ◽

Cited By ~ 19

Author(s):

A. Al-Nehlawi ◽

S. Guri ◽

B. Guamis ◽

J. Saldo

Keyword(s):

Carbon Dioxide ◽

Hydrostatic Pressure ◽

Synergistic Effect ◽

High Hydrostatic Pressure ◽

Spoilage Bacteria

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Combined Effect of High Hydrostatic Pressure and Nisin on Loss of Viability, Membrane Damage and Release of Intracellular Contents of Bacillus subtilis and Escherichia coli

International Journal of Food Engineering ◽

10.2202/1556-3758.1873 ◽

2010 ◽

Vol 6 (5) ◽

Cited By ~ 1

Author(s):

Wei-Min Qi ◽

Ping Qian ◽

Jian-Yong Yu ◽

Chi-Yu Zhang ◽

Xiao Chen ◽

...

Keyword(s):

Escherichia Coli ◽

Bacillus Subtilis ◽

Hydrostatic Pressure ◽

Synergistic Effect ◽

High Hydrostatic Pressure ◽

Cell Envelope ◽

Membrane Damage ◽

Combined Effect ◽

Gram Negative Bacteria ◽

E Coli

Bacillus subtilis and Escherichia coli were chosen to investigate the combined effect of high hydrostatic pressure (HHP) and Nisin on loss of viability, membrane damage and release of intracellular contents of microorganisms. The results showed that the combination of 200 IU/mL Nisin and HHP exhibited a synergistic effect over 2 log on the inactivation of B. subtilis at pressure 300 MPa. The similar synergistic effect was observed on the membrane damage and release of intracellular contents of B. subtilis. The Nisin alone had no effect against E. coli, which belongs to gram negative bacteria. However, at pressure 300 MPa, Nisin caused the membrane damage from 55% to 80%. The synergistic effect of Nisin and HHP on loss of viability, membrane damage and release of intracellular contents of E. coli were also illustrated when the HHP pressure exceeded 300 MPa as the consequence of the serious changes produced by HHP at higher pressure in the cell envelope. It allows the entry of Nisin molecules to cell membrane.

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