Estrogen Receptor-beta Mediates the Protective Effects of Aromatase Induction in the MMTV-Her-2/neu x Aromatase Double Transgenic Mice

2011 ◽  
Vol 3 (1-2) ◽  
pp. 26-36 ◽  
Author(s):  
Hareesh B. Nair ◽  
Rao P. Perla ◽  
Nameer B. Kirma ◽  
Naveen K. Krishnegowda ◽  
Manonmani Ganapathy ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Transgenic Mice ◽  
Estrogen Receptor Beta ◽  
Protective Effects ◽  
Her 2 ◽  
Double Transgenic Mice ◽  
Receptor Beta

Corrigendum to “Distribution of estrogen receptor beta containing cells in the brains of bacterial artificial chromosome transgenic mice” [Brain Res. 1351 (2010) 74–96]

2011 ◽  
Vol 1396 ◽  
pp. 105
Author(s):  
Teresa A. Milner ◽  
Louisa I. Thompson ◽  
Gang Wang ◽  
Justin A. Kievits ◽  
Eugene Martin ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Bacterial Artificial Chromosome ◽  
Transgenic Mice ◽  
Estrogen Receptor Beta ◽  
Artificial Chromosome ◽  
Mice Brain ◽  
Receptor Beta

Detection and Characterization of Estrogen Receptor Beta Expression in the Brain with Newly Developed Transgenic Mice

Neuroscience ◽  
2020 ◽  
Vol 438 ◽  
pp. 182-197
Author(s):  
Shoko Sagoshi ◽  
Sho Maejima ◽  
Masahiro Morishita ◽  
Satoshi Takenawa ◽  
Akito Otubo ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Transgenic Mice ◽  
Estrogen Receptor Beta ◽  
The Brain ◽  
Receptor Beta

scholarly journals Distribution of estrogen receptor beta containing cells in the brains of bacterial artificial chromosome transgenic mice

2010 ◽  
Vol 1351 ◽  
pp. 74-96 ◽  
Author(s):  
Teresa A. Milner ◽  
Louisa I. Thompson ◽  
Gang Wang ◽  
Justin A. Kievits ◽  
Eugene Martin ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Bacterial Artificial Chromosome ◽  
Transgenic Mice ◽  
Estrogen Receptor Beta ◽  
Artificial Chromosome ◽  
Receptor Beta

scholarly journals Estrogen Receptor Beta Influences the Inflammatory p65 Cistrome in Colon Cancer Cells

2021 ◽  
Vol 12 ◽  
Author(s):  
Rajitha Indukuri ◽  
Linnea Hases ◽  
Amena Archer ◽  
Cecilia Williams
Keyword(s):  
Estrogen Receptor ◽  
Cell Lines ◽  
Estrogen Receptor Beta ◽  
Estrogen Signaling ◽  
Chromatin Interaction ◽  
Protective Effects ◽  
Inflammatory Signaling ◽  
Necrosis Factor Alpha ◽  
First Time ◽  
Receptor Beta

Inflammation is a primary component of both initiation and promotion of colorectal cancer (CRC). Cytokines secreted by macrophages, including tumor necrosis factor alpha (TNFα), activates the pro-survival transcription factor complex NFκB. The precise mechanism of NFκB in CRC is not well studied, but we recently reported the genome-wide transcriptional impact of TNFα in two CRC cell lines. Further, estrogen signaling influences inflammation in a complex manner and suppresses CRC development. CRC protective effects of estrogen have been shown to be mediated by estrogen receptor beta (ERβ, ESR2), which also impacts inflammatory signaling of the colon. However, whether ERβ impacts the chromatin interaction (cistrome) of the main NFκB subunit p65 (RELA) is not known. We used p65 chromatin immunoprecipitation followed by sequencing (ChIP-Seq) in two different CRC cell lines, HT29 and SW480, with and without expression of ERβ. We here present the p65 colon cistrome of these two CRC cell lines. We identify that RELA and AP1 motifs are predominant in both cell lines, and additionally describe both common and cell line-specific p65 binding sites and correlate these to transcriptional changes related to inflammation, migration, apoptosis and circadian rhythm. Further, we determine that ERβ opposes a major fraction of p65 chromatin binding in HT29 cells, but enhances p65 binding in SW480 cells, thereby impacting the p65 cistrome differently in the two cell lines. However, the biological functions of the regulated genes appear to have similar roles in both cell lines. To our knowledge, this is the first time the p65 CRC cistrome is compared between different cell lines and the first time an influence by ERβ on the p65 cistrome is investigated. Our work provides a mechanistic foundation for a better understanding of how estrogen influences inflammatory signaling through NFκB in CRC cells.

Erratum to “Distribution of estrogen receptor beta containing cells in the brains of bacterial artificial chromosome transgenic mice” [Brain Res. 1351 (2010) 74–96]

2011 ◽  
Vol 1368 ◽  
pp. 361-362
Author(s):  
Teresa A. Milner ◽  
Louisa I. Thompson ◽  
Gang Wang ◽  
Justin A. Kievits ◽  
Eugene Martin ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Bacterial Artificial Chromosome ◽  
Transgenic Mice ◽  
Estrogen Receptor Beta ◽  
Artificial Chromosome ◽  
Mice Brain ◽  
Receptor Beta

scholarly journals Natural Herbal Estrogen-Mimetics (Phytoestrogens) Promote the Differentiation of Fallopian Tube Epithelium into Multi-Ciliated Cells via Estrogen Receptor Beta

Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 722
Author(s):  
Maobi Zhu ◽  
Sen Takeda ◽  
Tomohiko Iwano
Keyword(s):  
Estrogen Receptor ◽  
Cell Differentiation ◽  
Epithelial Cells ◽  
Fallopian Tube ◽  
Ciliated Cell ◽  
Estrogen Receptor Beta ◽  
Notch Pathway ◽  
Secretory Cells ◽  
Ciliated Cells ◽  
Receptor Beta

Phytoestrogens are herbal polyphenolic compounds that exert various estrogen-like effects in animals and can be taken in easily from a foodstuff in daily life. The fallopian tube lumen, where transportation of the oocyte occurs, is lined with secretory cells and multi-ciliated epithelial cells. Recently, we showed that estrogen induces multi-ciliogenesis in the porcine fallopian tube epithelial cells (FTECs) through the activation of the estrogen receptor beta (ERβ) pathway and simultaneous inhibition of the Notch pathway. Thus, ingested phytoestrogens may induce FTEC ciliogenesis and thereby affect the fecundity. To address this issue, we added isoflavones (genistein, daidzein, or glycitin) and coumestan (coumestrol) to primary culture FTECs under air–liquid interface conditions and assessed the effects of each compound. All phytoestrogens except glycitin induced multi-ciliated cell differentiation, which followed Notch signal downregulation. On the contrary, the differentiation of secretory cells decreased slightly. Furthermore, genistein and daidzein had a slight effect on the proportion of proliferating cells exhibited by Ki67 expression. Ciliated-cell differentiation is inhibited by the ERβ antagonist, PHTPP. Thus, this study suggests that phytoestrogens can improve the fallopian tube epithelial sheet homeostasis by facilitating the genesis of multi-ciliated cells and this effect depends on the ERβ-mediated pathway.

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