Development of nuclear microsatellite markers for the Tasmanian endemic conifer Diselma archeri Hook. F. (Cupressaceae)

2014 ◽  
Vol 7 (2) ◽  
pp. 369-372 ◽  
Author(s):  
Shota Sakaguchi ◽  
James R. P. Worth ◽  
Gregory J. Jordan ◽  
Philip Thomas ◽  
Motomi Ito
2015 ◽  
Vol 3 (12) ◽  
pp. 1500088 ◽  
Author(s):  
Juan Viruel ◽  
Pedro L. Ortiz ◽  
Montserrat Arista ◽  
María Talavera

Author(s):  
Dainis Edgars Ruņģis ◽  
Baiba Krivmane

Abstract Acer platanoides L. (Norway maple) is the most widespread native maple species in Europe, with a distribution from south and central Europe to northern Europe and Scandinavia. Acer platanoides is widespread throughout the territory of Latvia, and is mainly found in mixed broadleaf and conifer stands. The genetic diversity and differentiation of Latvian A. platanoides populations was analysed. Sampled populations were located throughout the territory of Latvia, and were selected to represent a range of ecological conditions, with differing levels of anthropogenic impact. A total of 496 individuals from 21 populations were analysed with eight microsatellite markers, which were developed from related Acer species. The obtained molecular data revealed a moderate level of polymorphism, and the analysed Latvian A. platanoides populations were moderately differentiated. This study provides an initial assessment of the genetic diversity and differentiation of Latvian A. platanoides populations, and is also one of the first reports of the analysis of A. platanoides populations using microsatellite markers. The results can be utilised to define A. platanoides genetic resource stands to ensure conservation of a wide range of germplasm.


2018 ◽  
Vol 65 (5) ◽  
pp. 1337-1348 ◽  
Author(s):  
Margherita Rodolfi ◽  
Tommaso Ganino ◽  
Benedetta Chiancone ◽  
Raffaella Petruccelli

2011 ◽  
Vol 59 (8) ◽  
pp. 749 ◽  
Author(s):  
Sarah Zanon Agapito-Tenfen ◽  
Neusa Steiner ◽  
Miguel Pedro Guerra ◽  
Rubens Onofre Nodari

The development of polyembryony is a common reproductive strategy in conifers. Multiple embryos are observed during early seed developmental stages. However, upon seed maturation, only the dominant embryo survives, with few exceptions. Although programmed cell death has been reported as the major mechanism responsible for elimination of subordinate embryos, the genetics of surviving embryos and the probabilities of survival remain unclear. The aim of this study is to determine patterns of polyembryony and survival frequency in Araucaria angustifolia (Bert) O. Ktze. Thus, we investigate the morphogenetic parameters that might be related to embryo survival using nuclear microsatellite markers and morphological characteristics of immature embryos and seedlings. Our novel approach couples genotype frequency analysis with the number of surviving embryos, presence of embryo dominance and number of cotyledons present within a single seed. Polyembryonic seedling frequency was low (0.022%) and 91% of surviving embryos were monozygotic. From all monozygotic embryos, 98% showed differences in growth rate (height) in relation to each other. Concrescent tissues were common in the monozygotic polyembryony patterns observed (80%) but not for those with polyzygotic polyembryony. We demonstrate that the survival of multiple embryos is a rare event in A. angustifolia seeds. To the best of our knowledge this study represents the first evidence of cleavage polyembryony in immature embryos and seedlings from A. angustifolia. Our novel approach using a combined set of morphological parameters and microsatellite markers was successful in investigating polyembryony patterns and survival.


2014 ◽  
Vol 2 (12) ◽  
pp. 1400093 ◽  
Author(s):  
Maria von Cräutlein ◽  
Helena Korpelainen ◽  
Marjo Helander ◽  
Annika Öhberg ◽  
Kari Saikkonen

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