scholarly journals Assessment of the Structure and Diversity of Latvian Acer platanoides Populations Using Cross-Species Nuclear Microsatellite Markers

2021 ◽  
Vol 75 (4) ◽  
pp. 254-260
Author(s):  
Dainis Edgars Ruņģis ◽  
Baiba Krivmane
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Genetic Resource ◽  
Molecular Data ◽  
Initial Assessment ◽  
Northern Europe ◽  
Acer Platanoides ◽  
Norway Maple ◽  
Wide Range ◽  
Nuclear Microsatellite

Abstract Acer platanoides L. (Norway maple) is the most widespread native maple species in Europe, with a distribution from south and central Europe to northern Europe and Scandinavia. Acer platanoides is widespread throughout the territory of Latvia, and is mainly found in mixed broadleaf and conifer stands. The genetic diversity and differentiation of Latvian A. platanoides populations was analysed. Sampled populations were located throughout the territory of Latvia, and were selected to represent a range of ecological conditions, with differing levels of anthropogenic impact. A total of 496 individuals from 21 populations were analysed with eight microsatellite markers, which were developed from related Acer species. The obtained molecular data revealed a moderate level of polymorphism, and the analysed Latvian A. platanoides populations were moderately differentiated. This study provides an initial assessment of the genetic diversity and differentiation of Latvian A. platanoides populations, and is also one of the first reports of the analysis of A. platanoides populations using microsatellite markers. The results can be utilised to define A. platanoides genetic resource stands to ensure conservation of a wide range of germplasm.

scholarly journals Molecular Diversity Analysis of Jattal and Dera Din Panah Goat Breeds of Pakistan using Microsatellite Markers

2018 ◽  
Vol 69 (1) ◽  
pp. 791 ◽  
Author(s):  
T. HUSSAIN ◽  
M. SHAHEEN ◽  
M. E. BABAR ◽  
M. M. MUSTHAFA ◽  
A. NADEEM ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Genetic Resource ◽  
Polymorphic Information Content ◽  
Goat Breed ◽  
Expected Heterozygosity ◽  
Animal Genetic Resource ◽  
Wide Range ◽  
The Mean ◽  
Distinct Features

This paper evaluates the genetic diversity of goat breeds in Pakistan, a country blessed with a wide range of goats spread throughout with distinct features contributing to a rich Animal Genetic Resource. The genetic diversity of two goat breeds (Jattal and Dera Din Panah) was assessed with 25 animals representing the two breeds using 16 microsatellite markers. The mean observed and expected heterozygosity of both goat breed populations were observed as 0.83+0.21. The average number of observed alleles was 3.6+1.6 for all loci. The mean polymorphic information content for a goat breed was 0.45, indicating the usefulness of markers panel. Highest Nei’s standard genetic distance (Ds) value of 0.0612 was observed between Jattal and Dera Din Panah goats, and the mean Fst value was 0.013. The measures of genetic variation revealed a good scope for effective improvement, conservation, and designing national breeding policies, in future, for Pakistan goat breeds.

scholarly journals The role of environment, local adaptation and past climate fluctuation on the amount and distribution of genetic diversity in the teosinte in Mexico

2019 ◽  
Author(s):  
Jaime Gasca-Pineda ◽  
Yocelyn T. Gutiérrez-Guerrero ◽  
Erika Aguirre-Planter ◽  
Luis E. Eguiarte
Keyword(s):  
Genetic Diversity ◽  
Local Adaptation ◽  
Environmental Conditions ◽  
Wide Distribution ◽  
Wide Range ◽  
Significant Genetic Structure ◽  
Nuclear Microsatellite ◽  
Genetic Clusters ◽  
Assignment Analysis ◽  
Climate Fluctuation

AbstractWild maize, commonly known as teosinte, has a wide distribution in central Mexico and inhabits a wide range of environmental conditions. According to previous studies, the environment is a determinant factor for the amount and distribution of genetic diversity. In this study, we used a set of neutral markers to explore the influence of contemporary factors and historical environmental shifts on genetic diversity, including present and three historical periods. Using a set of 22 nuclear microsatellite loci, we genotyped 527 individuals from 29 localities. We found highly variable levels of genetic diversity (Z. m. parviglumis HE= 0.3646–0.7699; Z. m. mexicana HE= 0.5885–0.7671) and significant genetic structure among localities (average DEST= 0.4332). Also, we recovered significant values of heterozygote deficiency (average FIS= 0.1796) and variable levels of selfing (sg2=0.0–0.3090). The Bayesian assignment analysis yielded four genetic clusters dividing the sample into subspecies, that in turn, were separated into two clusters. Environmental conditions played a strong influence in the distribution of genetic diversity, as demographic analysis and changes in species range revealed by modeling analyses were consistent. We conclude that current genetic diversity in teosinte is the result of a mixture of local adaptation and genetic isolation along with historical environmental fluctuations.

scholarly journals Short Note: Isolation and Characterization of 12 Polymorphic Microsatellite Markers in Engelhardia roxburghiana (Juglandaceae)

2014 ◽  
Vol 63 (1-6) ◽  
pp. 109-112 ◽  
Author(s):  
Dan-Dan Zhang ◽  
Pi Luo ◽  
Ying Chen ◽  
Zheng-Feng Wang ◽  
Wan-Hui Ye ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Short Note ◽  
Screening Method ◽  
Wide Distribution ◽  
Enriched Library ◽  
Isolation And Characterization ◽  
Nuclear Microsatellite ◽  
Polymorphic Microsatellite

Abstract Engelhardia roxburghiana is a common half evergreen tree with a wide distribution in southeast Asia. Despite its ecological and pharmaceutical values, its genetic diversity is poorly studied. Our objective was to develop nuclear microsatellite markers to investigate the level of genetic diversity within and among populations in the future. Using the microsatellite-enriched library and PCR-based screening method, 12 microsatellite markers were developed and showed polymorphism in a population. The number of alleles per locus for these 12 microsatellites ranged from four to 15. The observed and expected heterozygosities ranged from 0.358 to 0.897 and from 0.369 to 0.886, respectively. The developed microsatellites will be useful for studying genetic diversity and population structure in E. roxburghiana.

scholarly journals Isolation and Characterization of 88 Polymorphic Microsatellite Markers in Kentucky Bluegrass (Poa pratensis L.)

HortScience ◽  
2010 ◽  
Vol 45 (11) ◽  
pp. 1759-1763 ◽  
Author(s):  
Josh A. Honig ◽  
Stacy A. Bonos ◽  
William A. Meyer
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Rapd Markers ◽  
Poa Pratensis ◽  
Morphological Characteristics ◽  
Kentucky Bluegrass ◽  
Ploidy Levels ◽  
Isolation And Characterization ◽  
Wide Range ◽  
Polymorphic Microsatellite

Kentucky bluegrass (Poa pratensis L.) is an important facultative apomictic temperate perennial grass species used for both forage and cultivated turf. Through apomixis, this species is able to propagate diverse and odd ploidy levels, resulting in many genetically distinct phenotypes. A wide range of diverse cultivars and accessions of kentucky bluegrass have been previously characterized based on common turf performance or morphological characteristics as well as by random amplified polymorphic DNA (RAPD) markers. Although previous characterization efforts have provided valuable information, the use of both morphological characteristics and RAPD markers for genetic diversity analysis has limitations. In the current report, we developed and characterized 88 novel microsatellite markers for kentucky bluegrass. Polymorphism for each marker was assessed in 265 kentucky bluegrass cultivars, experimental selections, collections, and hybrids. The number of alleles for individual microsatellites ranged from four to 81 with an average of 38.3 alleles per simple sequence repeat. These polymorphic microsatellite markers would be useful tools for investigating genetic diversity, creation of genetic linkage maps, assessment of levels of apomixis in cultivars and experimental varieties, and identification of aberrant progeny in apomictic kentucky bluegrass breeding programs.

scholarly journals Variasi Genetik Itik Bayang Berbasis Marka Mikrosatelit Pada Lokus AY287 dan Lokus AY283

2017 ◽  
Vol 11 (2) ◽  
pp. 91 ◽  
Author(s):  
Rusfidra Rusfidra ◽  
Y. Heryandi ◽  
Jamsari Jamsari ◽  
E. Y. Rahman
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Key Words ◽  
Genetic Markers ◽  
Microsatellite Loci ◽  
Gel Electrophoresis ◽  
Genetic Resource ◽  
Allele Frequencies ◽  
Blood Samples

West Sumatera Province has poultry genetic resource of local duck that potential in supply<br />eggs and duck meat. Bayang duck was set by Indonesian Agricultural Ministry in 2012 as national livestock breeds in Indonesia. Microsatellite markers are widely used as a genetic identifier because of their abundant existence, co-dominant and high polymorphic. The purpose of this study was to determine the genetic diversity of Bayang ducks based on two microsatellite loci which include AY287 and AY283. DNA substances used in the study were blood samples from 24 Bayang duck in Pesisir Selatan Regency. The isolated DNA genom from 24 blood samples of Bayang duck could be detected by gel electrophoresis. Results showed that AY287 locus has 6 alleles; allele A (108 bp), allele B (142 bp), allele C (183 bp), allele D (227 bp), allele E (272 bp) and allele F (340 bp). Both allele E and F were specific genetic markers of Bayang duck. Alleles frequencies of the AY287 locus were as follow: allele C (26,93%), allele D (19,24%), allele A (15,38%), allele B (15,38%), allele E (15,38%) and allele F (7,69%). The AY283 locus has 6 alleles consisted of allele A (230 bp), allele B (320 bp), allele C (345 bp), allele D (390 bp), allele E (450 bp) and allele F (500 bp). Allele frequencies of this marker were allele B (20,51%), allele D (20,51%), allele E (20,51%), allele A (15,39%), allele C (15,39%), and allele F (7,69%), respectively. Our finding suggest that two microsatellite markers, AY287 and AY283, were polymorphic in Bayang duck population.<br />Key words: Bayang duck, microsatellite, AY283, AY287

scholarly journals Legume Genetic Resource Security as Main Requirement for Future Challenges

2021 ◽  
Author(s):  
Ifeoluwa Odesina ◽  
Nenyinka Gonzuk ◽  
Elizabeth Daodu ◽  
Sheyi Akintunde
Keyword(s):  
Genetic Diversity ◽  
Genetic Resource ◽  
Anthropogenic Activity ◽  
Crop Species ◽  
Main Requirement ◽  
Global Challenges ◽  
Wide Range ◽  
The World ◽  
Future Challenges ◽  
Growing Population

Evaluating the genetic diversity of landraces has exposed us to the diverse relevance of wild line contributory to a wide range of systems ranging from morphology, physiology, biochemistry, anatomy, toxicity, etc., allowing to their genetic constituent. Today, the world is facing many global challenges. This has put the world in disarray and poses a threat via its impact leading to non-promising food security for a rapidly growing population, an increase in the production and release of greenhouse gases as a consequence of anthropogenic activity, and an increase in the level of pollutants in the environment. A well-characterized crop genetic resource is a precondition for effective breeding and genetic conservation in the world of legume security. There is a need to collect, study and conserve legume genetic resource to tackle future challenges. This will help project latent benefits of undescribed leguminous lines of various crop species.

scholarly journals Genetic diversity analysis based on morphological characters in mulberry (Morus spp.)

2019 ◽  
Vol 28 ◽  
pp. 111-119
Author(s):  
MS Rahman ◽  
SMS Islam
Keyword(s):  
Genetic Diversity ◽  
Genetic Resource ◽  
Morphological Characters ◽  
Silk Production ◽  
Wide Range ◽  
Leaf Yield ◽  
Breeding Programmes ◽  
Morus Spp ◽  
Growth Attributes ◽  
Leaf Productivity

Mulberry genetic resource is increasingly being recognized as one of the basic key component for sustainable silk production under changing climatic condition. In this investigation, analysis of multivariate was done to assess the diversity in 20 mulberry genotypes (includes indigenous and exotic) for leaf yield and its growth attributes. The analysis of variance (ANOVA) showed the presence of significant variation among genotypes for the parameters measured. Wide range and variance among the genotypes indicated the presence of variability for the traits on which selection can be practiced. For cluster analysis classified 20 genotypes into four divergent groups and greater genetic distance was detected among the members of cluster I and II and cluster II and III. The members of these divergent clusters may be combined in future breeding programmes to obtain genotypes with combined leaf yield and more branches per plant. The results showed that the germplasm having a wide genetic diversity thus the genotypes viz., BSRM64, BSRM66, BSRM63, BSRM65, BSRM45 and BSRM56 can serve as a promising donors for improving the leaf productivity of mulberry. J. bio-sci. 28: 111-119, 2020

scholarly journals Norway maple (Acer platanoides) and pedunculate oak (Quercus robur) demonstrate different patterns of genetic variation within and among populations on the eastern border of distribution ranges

2021 ◽  
Vol 67 (No. 11) ◽  
pp. 522-532
Author(s):  
Artur Akhmetov ◽  
Ruslan Ianbaev ◽  
Svetlana Boronnikova ◽  
Yulai Yanbaev ◽  
Aygul Gabitova ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Genetic Differentiation ◽  
Population Genetic ◽  
Quercus Robur ◽  
Distribution Area ◽  
Pedunculate Oak ◽  
Acer Platanoides ◽  
Norway Maple ◽  
Key Species

Norway maple (Acer platanoides L.) is a key species of broadleaved forests whose population genetics is poorly studied using modern genetic tools. We used ISSR analysis to explore genetic diversity and differentiation among 10 Russian populations on the eastern margin of the species range of distribution, and to compare the revealed patterns with the results of our population genetic studies of pedunculate oak (Quercus robur L.). In the first set comparatively high heterozygosity and allelic diversity were found (expected heterozygosity H<sub>E</sub> = 0.160 ± 0.033, number of alleles n<sub>a</sub> = 1.440 ± 0.080, effective number of alleles n<sub>e</sub> = 1.271 ± 0.062) in comparison with strongly fragmented and geographically isolated small maple stands of the second set (H<sub>E</sub> = 0.083 ± 0.011, n<sub>a</sub> = 1.281 ± 0.031, n<sub>e</sub> = 1.136 ± 0.019). A relatively high genetic differentiation among populations was detected (the proportion of the inter-population component of total genetic variation, G<sub>ST</sub> = 0.558 ± 0.038). In the Cis-Urals, local groups of populations that are confined to the northern, middle and southern parts of the Urals were identified. On the contrary, the current significant fragmentation of the pedunculate oak distribution area in the same study area did not lead to any noticeable genetic differentiation among the majority of populations, the values of the population genetic diversity were very similar in different parts of the Southern Urals.

scholarly journals Variasi Genetik Itik Bayang Berbasis Marka Mikrosatelit Pada Lokus AY287 dan Lokus AY283

2017 ◽  
Vol 11 (2) ◽  
pp. 91
Author(s):  
Rusfidra Rusfidra ◽  
Y. Heryandi ◽  
Jamsari Jamsari ◽  
E. Y. Rahman
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Key Words ◽  
Genetic Markers ◽  
Microsatellite Loci ◽  
Gel Electrophoresis ◽  
Genetic Resource ◽  
Allele Frequencies ◽  
Blood Samples

West Sumatera Province has poultry genetic resource of local duck that potential in supply<br />eggs and duck meat. Bayang duck was set by Indonesian Agricultural Ministry in 2012 as national livestock breeds in Indonesia. Microsatellite markers are widely used as a genetic identifier because of their abundant existence, co-dominant and high polymorphic. The purpose of this study was to determine the genetic diversity of Bayang ducks based on two microsatellite loci which include AY287 and AY283. DNA substances used in the study were blood samples from 24 Bayang duck in Pesisir Selatan Regency. The isolated DNA genom from 24 blood samples of Bayang duck could be detected by gel electrophoresis. Results showed that AY287 locus has 6 alleles; allele A (108 bp), allele B (142 bp), allele C (183 bp), allele D (227 bp), allele E (272 bp) and allele F (340 bp). Both allele E and F were specific genetic markers of Bayang duck. Alleles frequencies of the AY287 locus were as follow: allele C (26,93%), allele D (19,24%), allele A (15,38%), allele B (15,38%), allele E (15,38%) and allele F (7,69%). The AY283 locus has 6 alleles consisted of allele A (230 bp), allele B (320 bp), allele C (345 bp), allele D (390 bp), allele E (450 bp) and allele F (500 bp). Allele frequencies of this marker were allele B (20,51%), allele D (20,51%), allele E (20,51%), allele A (15,39%), allele C (15,39%), and allele F (7,69%), respectively. Our finding suggest that two microsatellite markers, AY287 and AY283, were polymorphic in Bayang duck population.<br />Key words: Bayang duck, microsatellite, AY283, AY287

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