scholarly journals Molecular characterization, modeling, and docking analysis of late phytic acid biosynthesis pathway gene, inositol polyphosphate 6-/3-/5-kinase, a potential candidate for developing low phytate crops

3 Biotech ◽  
2018 ◽  
Vol 8 (8) ◽  
Author(s):  
Mansi Punjabi ◽  
Navneeta Bharadvaja ◽  
Archana Sachdev ◽  
Veda Krishnan
Keyword(s):  
Molecular Characterization ◽  
Phytic Acid ◽  
Potential Candidate ◽  
Biosynthesis Pathway ◽  
Inositol Polyphosphate ◽  
Acid Biosynthesis ◽  
Docking Analysis ◽  
Pathway Gene ◽  
Kinase A

scholarly journals Hormonal Regulation and Expression Profiles of Wheat Genes Involved during Phytic Acid Biosynthesis Pathway

Plants ◽  
2015 ◽  
Vol 4 (2) ◽  
pp. 298-319 ◽  
Author(s):  
Sipla Aggarwal ◽  
Vishnu Shukla ◽  
Kaushal Bhati ◽  
Mandeep Kaur ◽  
Shivani Sharma ◽  
...  
Keyword(s):  
Phytic Acid ◽  
Hormonal Regulation ◽  
Expression Profiles ◽  
Biosynthesis Pathway ◽  
Acid Biosynthesis

scholarly journals Synthesis and Biological Evaluation of a Novel Glycidyl Metharcylate/Phaytic Acid-Based on Bagasse Xylan Composite Derivative

Polymers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 2084
Author(s):  
Mingkun Li ◽  
Heping Li ◽  
Hongli Liu ◽  
Zhiming Zou ◽  
Chaoyu Xie
Keyword(s):  
Lung Cancer ◽  
Thermal Stability ◽  
Ionic Liquid ◽  
Molecular Docking ◽  
Phytic Acid ◽  
Biological Activities ◽  
Biological Evaluation ◽  
Potential Candidate ◽  
Reaction Conditions ◽  
Cell Proliferation Inhibition

The development of natural biomass materials with excellent properties is an attractive way to improve the application range of natural polysaccharides. Bagasse Xylan (BX) is a natural polysaccharide with various biological activities, such as antitumor, antioxidant, etc. Its physic-chemical and biological properties can be improved by functionalization. For this purpose, a novel glycidyl metharcylate/phytic acid based on a BX composite derivative was synthesized by a free radical polymerization technique with glycidyl metharcylate (GMA; GMABX) and further esterification with phytic acid (PA; GMABX-PA) in ionic liquid. The effects of the reaction conditions (i.e., temperature, time, initiator concentration, catalyst concentration, GMA concentration, PA concentration, mass of ionic liquid) on grafting rate(G), conversion rate(C) and degree of substitution(DS) are discussed. The structure of the composite material structure was confirmed by FTIR, 1H NMR and XRD. SEM confirmed the particle morphology of the composite derivative. The thermal stability of GMABX-PA was determined by TG-DTG. Molecular docking was further performed to study the combination mode of the GMABX-PA into the active site of two lung cancer proteins (5XNV, 2EB2) and a blood cancer protein (2M6N). In addition, tumor cell proliferation inhibition assays for BX, GMABX-PA were carried out using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetraz -olium bromide (MTT) method. The results showed that various reaction conditions exhibited favorable gradient curves, and that a maximum G of 56% for the graft copolymerization and a maximum DS of 0.267 can be achieved. The thermal stability was significantly improved, as demonstrated by the fact that there was still 60% residual at 800 °C. The molecular docking software generated satisfactory results with regard to the evaluated binding energy and combining sites. The inhibition ratio of GMABX-PA on NCI-H460 (lung cancer cells) reached 29.68% ± 4.45%, which is five times higher than that of BX. Therefore, the material was shown to be a potential candidate for biomedical applications as well as for use as a heat resistant material.

scholarly journals Analysis of the ergosterol biosynthesis pathway cloning, molecular characterization and phylogeny of lanosterol 14α-demethylase (ERG11) gene of Moniliophthora perniciosa

2014 ◽  
Vol 37 (4) ◽  
pp. 683-693 ◽  
Author(s):  
Geruza de Oliveira Ceita ◽  
Laurival Antônio Vilas-Boas ◽  
Marcelo Santos Castilho ◽  
Marcelo Falsarella Carazzolle ◽  
Carlos Priminho Pirovani ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Ergosterol Biosynthesis ◽  
Biosynthesis Pathway ◽  
Moniliophthora Perniciosa

scholarly journals Crystal Structure and Mechanistic Implications of N2-(2-Carboxyethyl)arginine Synthase, the First Enzyme in the Clavulanic Acid Biosynthesis Pathway

2003 ◽  
Vol 279 (7) ◽  
pp. 5685-5692 ◽  
Author(s):  
Matthew E. C. Caines ◽  
Jonathan M. Elkins ◽  
Kirsty S. Hewitson ◽  
Christopher J. Schofield
Keyword(s):  
Crystal Structure ◽  
Clavulanic Acid ◽  
Biosynthesis Pathway ◽  
Acid Biosynthesis

scholarly journals Reconstruction of the Fatty Acid Biosynthetic Pathway ofExiguobacterium antarcticumB7 Based on Genomic and Bibliomic Data

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Regiane Kawasaki ◽  
Rafael A. Baraúna ◽  
Artur Silva ◽  
Marta S. P. Carepo ◽  
Rui Oliveira ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fatty Acid Biosynthesis ◽  
De Novo ◽  
Short Chain Fatty Acids ◽  
Hexadecenoic Acid ◽  
Biosynthesis Pathway ◽  
Acid Biosynthesis ◽  
Cold Environments ◽  
Fatty Acid Biosynthesis Pathway

Exiguobacterium antarcticumB7 is extremophile Gram-positive bacteria able to survive in cold environments. A key factor to understanding cold adaptation processes is related to the modification of fatty acids composing the cell membranes of psychrotrophic bacteria. In our study we show thein silicoreconstruction of the fatty acid biosynthesis pathway ofE. antarcticumB7. To build the stoichiometric model, a semiautomatic procedure was applied, which integrates genome information using KEGG and RAST/SEED. Constraint-based methods, namely, Flux Balance Analysis (FBA) and elementary modes (EM), were applied. FBA was implemented in the sense of hexadecenoic acid production maximization. To evaluate the influence of the gene expression in the fluxome analysis, FBA was also calculated using thelog2⁡FCvalues obtained in the transcriptome analysis at 0°C and 37°C. The fatty acid biosynthesis pathway showed a total of 13 elementary flux modes, four of which showed routes for the production of hexadecenoic acid. The reconstructed pathway demonstrated the capacity ofE. antarcticumB7 tode novoproduce fatty acid molecules. Under the influence of the transcriptome, the fluxome was altered, promoting the production of short-chain fatty acids. The calculated models contribute to better understanding of the bacterial adaptation at cold environments.

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