Multi-walled carbon nanotubes improved growth, anatomy, physiology, secondary metabolism, and callus performance in Catharanthus roseus: an in vitro study

Engineered nanomaterials are potentially very useful for a variety of applications, but studies are needed to ascertain whether these materials pose a risk to human health. Here, we studied three benchmark nanomaterials (Ag nanoparticles, TiO2 nanoparticles, and multi-walled carbon nanotubes, MWCNTs) procured from the nanomaterial repository at the Joint Research Centre of the European Commission. Having established a sub-lethal concentration of these materials using two human cell lines representative of the immune system and the lungs, respectively, we performed RNA sequencing of the macrophage-like cell line after exposure for 6, 12, and 24 h. Downstream analysis of the transcriptomics data revealed significant effects on chemokine signaling pathways. CCR2 was identified as the most significantly upregulated gene in MWCNT-exposed cells. Using multiplex assays to evaluate cytokine and chemokine secretion, we could show significant effects of MWCNTs on several chemokines, including CCL2, a ligand of CCR2. The results demonstrate the importance of evaluating sub-lethal concentrations of nanomaterials in relevant target cells.

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Biocompatibility Characteristics of Titanium Coated with Multi Walled Carbon Nanotubes—Hydroxyapatite Nanocomposites

Materials ◽

10.3390/ma12020224 ◽

2019 ◽

Vol 12 (2) ◽

pp. 224 ◽

Cited By ~ 5

Author(s):

Jung-Eun Park ◽

Yong-Seok Jang ◽

Tae-Sung Bae ◽

Min-Ho Lee

Keyword(s):

Electron Microscopy ◽

Carbon Nanotubes ◽

Sol Gel ◽

Pure Titanium ◽

Cell Proliferation And Differentiation ◽

Transmission Electron ◽

Proliferation And Differentiation ◽

Multi Walled Carbon Nanotubes ◽

Walled Carbon Nanotubes

Multi walled carbon nanotubes-hydroxyapatite (MWCNTs-HA) with various contents of MWCNTs was synthesized using the sol-gel method. MWCNTs-HA composites were characterized by X-ray diffraction (XRD) and transmission electron microscopy (TEM). HA particles were generated on the surface of MWCNT. Produced MWCNTs-HA nanocomposites were coated on pure titanium (PT). Characteristic of the titanium coated MWCNTs-HA was evaluated by field-emission scanning electron microscopy (FE-SEM) and XRD. The results show that the titanium surface was covered with MWCNTs-HA nanoparticles and MWCNTs help form the crystalized hydroxyapatite. Furthermore, the MWCNTs-HA coated titanium was investigated for in vitro cellular responses. Cell proliferation and differentiation were improved on the surface of MWCNT-HA coated titanium.

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Bioactive poly(etheretherketone) composite containing calcium polyphosphate and multi-walled carbon nanotubes for bone repair: Mechanical property and in vitro biocompatibility

Journal of Bioactive and Compatible Polymers ◽

10.1177/0883911518783214 ◽

2018 ◽

Vol 33 (5) ◽

pp. 543-557 ◽

Cited By ~ 4

Author(s):

Jianfei Cao ◽

Yue Lu ◽

Hechun Chen ◽

Lifang Zhang ◽

Chengdong Xiong

Keyword(s):

Mechanical Property ◽

Carbon Nanotubes ◽

Bone Repair ◽

Mechanical Performance ◽

Injection Molding Process ◽

Molding Process ◽

Calcium Polyphosphate ◽

Multi Walled Carbon Nanotubes ◽

Walled Carbon Nanotubes

Poly(etheretherketone) exhibits good biocompatibility, excellent mechanical properties, and bone-like stiffness. However, the natural bio-inertness of pure poly(etheretherketone) hinders its applications in biomedical field, especially when direct bone-implant osteo-integration is desired. For developing an alternative biomaterial for load-bearing orthopedic application, combination of bioactive fillers with poly(etheretherketone) matrix is a feasible approach. In this study, a bioactive multi-walled carbon nanotubes/calcium polyphosphate/poly(etheretherketone) composite was prepared through a compounding and injection-molding process for the first time. Bioactive calcium polyphosphate was added to polymer matrix to enhance the bioactivity of the composite, and incorporation of multi-walled carbon nanotubes to composite was aimed to improve both the mechanical property and biocompatibility. Furthermore, the microstructures, surface hydrophilicity, and mechanical property of multi-walled carbon nanotubes/calcium polyphosphate/poly(etheretherketone) composite, as well as the cellular responses of MC3T3-E1 osteoblast cells to this material were investigated. The mechanical testing revealed that mechanical performance of the resulting ternary composite was significantly enhanced by adding the multi-walled carbon nanotubes and the mechanical values obtained were close to or higher than those of human cortical bone. More importantly, cell culture tests showed that initial cell adhesion, cell viability, and osteogenic differentiation of MC3T3-E1 cells were significantly promoted on the multi-walled carbon nanotubes/calcium polyphosphate/poly(etheretherketone) composite. Accordingly, the multi-walled carbon nanotubes/calcium polyphosphate/poly(etheretherketone) composite may be used as a promising bone repair material in dental and orthopedic applications.

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The addition of carbon nanotubes to orthodontic adhesives: an in vitro study

Australasian Orthodontic Journal ◽

10.21307/aoj-2020-085 ◽

2021 ◽

Vol 33 (1) ◽

pp. 57-63

Author(s):

Paulo Guilherme Bittencourt Marchi ◽

Felipe de Brum Ricardi ◽

Maurício Matté Zanini ◽

Paulo Rodrigo Stival Bittencourt ◽

Mauro Carlos Agner Busato

Keyword(s):

Carbon Nanotubes ◽

In Vitro Study ◽

Vitro Study ◽

Orthodontic Adhesives

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Assessment of the dermal and ocular irritation potential of multi-walled carbon nanotubes by using in vitro and in vivo methods

Toxicology Letters ◽

10.1016/j.toxlet.2009.09.007 ◽

2009 ◽

Vol 191 (2-3) ◽

pp. 268-274 ◽

Cited By ~ 50

Author(s):

A. Sairam Kishore ◽

P. Surekha ◽

P. Balakrishna Murthy

Keyword(s):

Carbon Nanotubes ◽

Ocular Irritation ◽

Multi Walled Carbon Nanotubes ◽

Walled Carbon Nanotubes

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Biodegradable multi-walled carbon nanotubes trigger anti-tumoral effects

Nanoscale ◽

10.1039/c8nr03036g ◽

2018 ◽

Vol 10 (23) ◽

pp. 11013-11020 ◽

Cited By ~ 11

Author(s):

E. González-Lavado ◽

N. Iturrioz-Rodríguez ◽

E. Padín-González ◽

J. González ◽

L. García-Hevia ◽

...

Keyword(s):

Carbon Nanotubes ◽

Malignant Melanoma ◽

Solid Tumors ◽

Mild Oxidation ◽

Multi Walled Carbon Nanotubes ◽

Walled Carbon Nanotubes

Mild oxidation treatments improve the in vitro and in vivo macrophage biodegradation of carbon nanotubes that trigger remarkable anti-tumoral effects in malignant melanoma solid tumors produced in mice.

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Assessment of the antibacterial activity of Catharanthus roseus leaf extract on periodontal pathogens, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Prevotella intermedia: An In vitro study

Journal of Interdisciplinary Dentistry ◽

10.4103/jid.jid_1_20 ◽

2020 ◽

Vol 10 (2) ◽

pp. 61

Author(s):

KhushbooVijaysinh Rathod ◽

Swetalin Das

Keyword(s):

Antibacterial Activity ◽

Porphyromonas Gingivalis ◽

Catharanthus Roseus ◽

Leaf Extract ◽

In Vitro Study ◽

Aggregatibacter Actinomycetemcomitans ◽

Vitro Study ◽

Prevotella Intermedia ◽

Periodontal Pathogens

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The Application of Multi-Walled Carbon Nanotubes in Bone Tissue Repair Hybrid Scaffolds and the Effect on Cell Growth In Vitro

Polymers ◽

10.3390/polym11020230 ◽

2019 ◽

Vol 11 (2) ◽

pp. 230 ◽

Cited By ~ 5

Author(s):

Jie Xu ◽

Xueyan Hu ◽

Siyu Jiang ◽

Yiwei Wang ◽

Roxanne Parungao ◽

...

Keyword(s):

Carbon Nanotubes ◽

Bone Tissue ◽

Optimal Growth ◽

Bone Tissue Regeneration ◽

Proliferation And Differentiation ◽

Potential Applications ◽

Multi Walled Carbon Nanotubes ◽

Walled Carbon Nanotubes ◽

Hybrid Scaffolds

In this study, composite scaffolds with different multi-walled carbon nanotubes (MWCNTs) content were prepared by freeze-drying. These scaffolds were characterized by scanning electron microscope (SEM), energy dispersive spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR), porosity, hydrophilicity, mechanical strength, and degradation. The MWCNTs scaffolds were structurally sound and had porous structures that offered ample space for adherence, proliferation, and differentiation of MC3T3-E1 cells, and also supported the transport of nutrients and metabolic waste. CS/Gel/nHAp/0.3%MWCNTs scaffolds provided the best outcomes in terms of scaffold porosity, hydrophilicity, and degradation rate. However, CS/Gel/nHAp/0.6%MWCNTs scaffolds were found to support the optimal growth, homogenous distribution, and biological activity of MC3T3-E1 cells. The excellent properties of CS/Gel/nHAp/0.6%MWCNTs scaffolds for the adhesion, proliferation, and osteogenesis differentiation of MC3T3-E1 cells in vitro highlights the potential applications of this scaffold in bone tissue regeneration.

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