Glycated albumin (GA) and the GA/HbA1c ratio are higher in diabetic patients positive for insulin antibodies with high binding capacity and low affinity

Abstract Diabetic patients receiving insulin therapy generally develop anti-insulin antibodies that must be eliminated, usually by extraction with polyethylene glycol (PEG), before determining the concentration of free (active) insulin in plasma. We describe a new method for removing such antibodies, with the use of Protein A coupled to Sepharose microspheres. The results correlate well with those by the PEG method, although values are systematically higher or lower for given samples, according to the initial titer of the antibody measured in terms of binding capacity. Further studies are required to clarify this observation.

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Magnetic Separation of Antibodies with High Binding Capacity by Site-Directed Immobilization of Protein A-Domains to Bare Iron Oxide Nanoparticles

ACS Applied Nano Materials ◽

10.1021/acsanm.1c00487 ◽

2021 ◽

Author(s):

Yasmin Kaveh-Baghbaderani ◽

Raphaela Allgayer ◽

Sebastian Patrick Schwaminger ◽

Paula Fraga-García ◽

Sonja Berensmeier

Keyword(s):

Iron Oxide ◽

Magnetic Separation ◽

Iron Oxide Nanoparticles ◽

Binding Capacity ◽

Protein A ◽

Oxide Nanoparticles ◽

High Binding ◽

A Domains

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A Novel Approach to Magnetic Nanoadsorbents with High Binding Capacity for Bovine Serum Albumin

Macromolecular Rapid Communications ◽

10.1002/marc.200600727 ◽

2007 ◽

Vol 28 (3) ◽

pp. 346-351 ◽

Cited By ~ 13

Author(s):

Yabin Sun ◽

Xiaobin Ding ◽

Zhaohui Zheng ◽

Xu Cheng ◽

Xinhua Hu ◽

...

Keyword(s):

Bovine Serum Albumin ◽

Serum Albumin ◽

Bovine Serum ◽

Binding Capacity ◽

High Binding ◽

Novel Approach

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The association of glycated albumin and hemoglobin A1c with glycemic excursions in Chinese type 2 diabetic patients

Diabetes Research and Clinical Practice ◽

10.1016/s0168-8227(16)31139-1 ◽

2016 ◽

Vol 120 ◽

pp. S92-S93

Author(s):

Qing Li ◽

Yuqian Bao ◽

Weiping Jia ◽

Jieming Pan ◽

Lei Zhang ◽

...

Keyword(s):

Hemoglobin A1c ◽

Glycated Albumin ◽

Diabetic Patients ◽

Type 2 Diabetic Patients ◽

Type 2 Diabetic

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Performance Characteristics of Glycated Albumin and Its Clinical Usefulness in Diabetic Patients on Hemodialysis

Annals of Laboratory Medicine ◽

10.3343/kjlm.2009.29.5.406 ◽

2009 ◽

Vol 29 (5) ◽

pp. 406-414 ◽

Cited By ~ 2

Author(s):

Hae-il Park ◽

Yeong Sic Kim ◽

Jehoon Lee ◽

Yonggoo Kim ◽

Seok Joon Shin

Keyword(s):

Glycated Albumin ◽

Performance Characteristics ◽

Clinical Usefulness ◽

Diabetic Patients

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Assessment of Hyperglycemia, Hypoglycemia and Inter-Day Glucose Variability Using Continuous Glucose Monitoring among Diabetic Patients on Chronic Hemodialysis

Journal of Clinical Medicine ◽

10.3390/jcm10184116 ◽

2021 ◽

Vol 10 (18) ◽

pp. 4116

Author(s):

Maria Divani ◽

Panagiotis I. Georgianos ◽

Triantafyllos Didangelos ◽

Vassilios Liakopoulos ◽

Kali Makedou ◽

...

Keyword(s):

Continuous Glucose Monitoring ◽

Glycated Albumin ◽

Glucose Monitoring ◽

Area Under The Curve ◽

Glucose Variability ◽

Hemodialysis Patients ◽

Chronic Hemodialysis ◽

Diabetic Patients ◽

Lower Efficiency ◽

Target Glucose

Continuous glucose monitoring (CGM) facilitates the assessment of short-term glucose variability and identification of acute excursions of hyper- and hypo-glycemia. Among 37 diabetic hemodialysis patients who underwent 7-day CGM with the iPRO2 device (Medtronic Diabetes, Northridge, CA, USA), we explored the accuracy of glycated albumin (GA) and hemoglobin A1c (HbA1c) in assessing glycemic control, using CGM-derived metrics as the reference standard. In receiver operating characteristic (ROC) analysis, the area under the curve (AUC) in diagnosing a time in the target glucose range of 70–180 mg/dL (TIR70–180) in <50% of readings was higher for GA (AUC: 0.878; 95% confidence interval (CI): 0.728–0.962) as compared to HbA1c (AUC: 0.682; 95% CI: 0.508–0.825) (p < 0.01). The accuracy of GA (AUC: 0.939; 95% CI: 0.808–0.991) in detecting a time above the target glucose range > 250 mg/dL (TAR>250) in >10% of readings did not differ from that of HbA1c (AUC: 0.854; 95% CI: 0.699–0.948) (p = 0.16). GA (AUC: 0.712; 95% CI: 0.539–0.848) and HbA1c (AUC: 0.740; 95% CI: 0.570–0.870) had a similarly lower efficiency in detecting a time below target glucose range < 70 mg/dL (TBR<70) in >1% of readings (p = 0.71). Although the mean glucose levels were similar, the coefficient of variation of glucose recordings (39.2 ± 17.3% vs. 32.0 ± 7.8%, p < 0.001) and TBR<70 (median (range): 5.6% (0, 25.8) vs. 2.8% (0, 17.9)) were higher during the dialysis-on than during the dialysis-off day. In conclusion, the present study shows that among diabetic hemodialysis patients, GA had higher accuracy than HbA1c in detecting a 7-day CGM-derived TIR70–180 < 50%. However, both biomarkers provided an imprecise reflection of acute excursions of hypoglycemia and inter-day glucose variability.

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