Analysis of a Plasmodium falciparum EBA-175 peptide with high binding capacity to erythrocytes and their analogues using 1H NMR

2003 ◽  
Vol 141 (2) ◽  
pp. 115-121 ◽  
Author(s):  
Gladys Cifuentes ◽  
Fanny Guzmán ◽  
Martha Patricia Alba ◽  
Luz Mary Salazar ◽  
Manuel Elkin Patarroyo
Keyword(s):  
Plasmodium Falciparum ◽  
1H Nmr ◽  
Binding Capacity ◽  
High Binding

Magnetic Separation of Antibodies with High Binding Capacity by Site-Directed Immobilization of Protein A-Domains to Bare Iron Oxide Nanoparticles

2021 ◽  
Author(s):  
Yasmin Kaveh-Baghbaderani ◽  
Raphaela Allgayer ◽  
Sebastian Patrick Schwaminger ◽  
Paula Fraga-García ◽  
Sonja Berensmeier
Keyword(s):  
Iron Oxide ◽  
Magnetic Separation ◽  
Iron Oxide Nanoparticles ◽  
Binding Capacity ◽  
Protein A ◽  
Oxide Nanoparticles ◽  
High Binding ◽  
A Domains

A Novel Approach to Magnetic Nanoadsorbents with High Binding Capacity for Bovine Serum Albumin

2007 ◽  
Vol 28 (3) ◽  
pp. 346-351 ◽  
Author(s):  
Yabin Sun ◽  
Xiaobin Ding ◽  
Zhaohui Zheng ◽  
Xu Cheng ◽  
Xinhua Hu ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Binding Capacity ◽  
High Binding ◽  
Novel Approach

Cyclic peptide ligand with high binding capacity for affinity purification of immunoglobulin G

2016 ◽  
Vol 1466 ◽  
pp. 105-112 ◽  
Author(s):  
Hyo Jin Kang ◽  
Weonu Choe ◽  
Jeong-Ki Min ◽  
Young-mi Lee ◽  
B. Moon Kim ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Cyclic Peptide ◽  
Binding Capacity ◽  
Affinity Purification ◽  
Peptide Ligand ◽  
High Binding

scholarly journals Developmental Stage- and Cell Cycle Number-Dependent Changes in Characteristics of Plasmodium falciparum-Infected Erythrocyte Adherence to Placental Chondroitin-4-Sulfate Proteoglycan

2007 ◽  
Vol 75 (9) ◽  
pp. 4409-4415 ◽  
Author(s):  
SubbaRao V. Madhunapantula ◽  
Rajeshwara N. Achur ◽  
D. Channe Gowda
Keyword(s):  
Plasmodium Falciparum ◽  
Binding Capacity ◽  
Protein S ◽  
Parasite Development ◽  
Trypsin Treatment ◽  
Cycle Number ◽  
Adhesive Protein ◽  
Parasite Stage ◽  
Selection For ◽  
Successive Generations

ABSTRACTThe adherence ofPlasmodium falciparum-infected red blood cells (IRBCs) in the human placenta is mediated by chondroitin-4-sulfate (C4S). Although IRBC binding to C4S has been unequivocally established, the adherence characteristics of IRBCs at different stages of parasite development and through successive parasite generations after selection for C4S adherence are not known. Here we show that IRBCs acquire a significant capacity to bind to C4S at as early as 14 h and exhibit maximum binding at 22 to 26 h postinvasion. Surprisingly, the IRBC binding ability decreases by ∼50% at the late trophozoite and schizont stages. The binding strength of the IRBCs also gradually decreases during successive generations after selection for C4S binding, and at the 32nd generation, the binding capacity was only ∼31% of that of IRBCs at the 2nd generation, suggesting that IRBCs eventually lose their C4S-adherent capacity. We also tested the susceptibility of the adhesive protein(s) on the IRBC surface to trypsin treatment at different stages of parasite development. The data show that IRBCs with late trophozoites are more resistant to trypsin treatment than those containing early trophozoites, indicating that parasite proteins expressed on the IRBC surface during trophozoite maturation partially mask accessibility of adhesive protein for binding to C4S. These data provide important insights into the expression pattern of the C4S-adhesive protein(s) on the IRBC surface, emphasizing the need for understanding the regulation of genes involved in IRBC binding to C4S. Our data also define the parasite stage at which IRBCs are suitable for studying structural interactions with C4S.

Ionic liquid modified magnetic microspheres for isolation of heme protein with high binding capacity

2013 ◽  
Vol 1 (15) ◽  
pp. 2066 ◽  
Author(s):  
Yun Wei ◽  
Yan Li ◽  
Ailin Tian ◽  
Yuntian Fan ◽  
Xiong Wang
Keyword(s):  
Ionic Liquid ◽  
Binding Capacity ◽  
Heme Protein ◽  
Magnetic Microspheres ◽  
High Binding
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