Rearing the 28-spotted ladybird beetle, Henosepilachna vigintioctopunctata (Coleoptera: Coccinelidae), with a switchover from host plant leaves to artificial diet

2014 ◽  
Vol 49 (2) ◽  
pp. 359-362 ◽  
Author(s):  
Kei Kawazu
2004 ◽  
Vol 39 (4) ◽  
pp. 537-544 ◽  
Author(s):  
Hanife Genc ◽  
James L. Nation

The Phaon crescent butterfly, Phyciodes phaon (Edwards), can be reared by placing newly-eclosed larvae on a pinto bean-based artificial diet; however, the adults fail to reproduce. Addition of 10% (w/w) of freeze-dried leaves of the host plant, Phyla nodiflora (L.) Greene, to the artificial diet approximately doubled the number of adults produced, and females oviposited viable eggs. Addition of wheat germ oil, linseed oil, or olive oil to the artificial diet without host plant tissue also increased survival to the adult stage, but adult females failed to oviposit. Larvae and adults reared from the artificial diet with addition of any one of the oils contained a higher quantity of linolenic acid in their body lipids than those reared on the artificial diet without additional oil. Leaves of the larval host plant are especially rich in the polyunsaturated fatty acids, linoleic acid and linolenic acid; whereas, the pinto bean diet is relatively low in these fatty acids, especially linolenic acid. Addition of synthetic β-sitosterol, stigmasterol, and campesterol, the sterols identified in the host plant leaves, to the artificial diet improved neither larval nor adult survival, and adult females did not oviposit. The factor (or factors) present in host plant leaves and acquired during larval feeding that enables reproduction of adults remains to be identified.


2021 ◽  
Vol 22 (11) ◽  
pp. 5941
Author(s):  
Abigail Ngugi-Dawit ◽  
Isaac Njaci ◽  
Thomas J.V. Higgins ◽  
Brett Williams ◽  
Sita R. Ghimire ◽  
...  

Pigeonpea [Cajanus cajan (L.) Millspaugh] is an economically important legume playing a crucial role in the semi-arid tropics. Pigeonpea is susceptible to Helicoverpa armigera (Hübner), which causes devastating yield losses. This pest is developing resistance to many commercially available insecticides. Therefore, crop wild relatives of pigeonpea, are being considered as potential sources of genes to expand the genetic base of cultivated pigeonpea to improve traits such as host plant resistance to pests and pathogens. Quantitative proteomic analysis was conducted using the tandem mass tag platform to identify differentially abundant proteins between IBS 3471 and ICPL 87 tolerant accession and susceptible variety to H. armigera, respectively. Leaf proteome were analysed at the vegetative and flowering/podding growth stages. H. armigera tolerance in IBS 3471 appeared to be related to enhanced defence responses, such as changes in secondary metabolite precursors, antioxidants, and the phenylpropanoid pathway. The development of larvae fed on an artificial diet with IBS 3471 lyophilised leaves showed similar inhibition with those fed on an artificial diet with quercetin concentrations with 32 mg/25 g of artificial diet. DAB staining (3,3’-diaminobenzidine) revealed a rapid accumulation of reactive oxygen species in IBS 3471. We conclude that IBS 3471 is an ideal candidate for improving the genetic base of cultivated pigeonpea, including traits for host plant resistance.


1993 ◽  
Vol 139 (9) ◽  
pp. 2251-2260 ◽  
Author(s):  
M. Romantschuk ◽  
E.-L. Nurmiaho-Lassila ◽  
E. Roine ◽  
A. Suoniemi

2019 ◽  
Vol 22 (4) ◽  
pp. 404-412
Author(s):  
Kei W. Matsubayashi ◽  
Sih Kahono ◽  
Sri Hartini ◽  
Haruo Katakura

1999 ◽  
Vol 91 (1) ◽  
pp. 245-250 ◽  
Author(s):  
Hideki Ueno ◽  
Naoyuki Fujiyama ◽  
Kiyoshi Irie ◽  
Yuji Sato ◽  
Haruo Katakura

Author(s):  
Rachappa V. ◽  
S. G. Hanchinal ◽  
Chandra Shekhara ◽  
Sweta Surpur ◽  
B. V. Patil ◽  
...  

The investigation on refinement and evaluation of artificial diet for M. vitrata, were carried out during 2014 and 2015 under walk in plant growth chamber established at ARS, Gulbarga, Karnataka, India. Comparison of developmental parameters of three successive generations of M. vitrata reared on different diets revealed that, maximum survival of egg, larva and pupa was 78.4 ± 3.1, 67.6±5.5 and 73.0±5.0 respectively in modified D-OOD diet and was superior over rest of the diets (D-OOD, D-New and D-OO) while it was on par with natural host plant. Maximum fecundity of 62.34±4.1 eggs/female was observed in refined D-OOD diet was on par with pigeonpea host plant and superior over other diets. Biology studies of Maruca on refined artificial diet in comparison with natural host plant pigonpea were revealed that, incubation period (2.6 ± 0.46 and 2.85 ± 0.40 days), total larval duration (14.28± 1.02 and 14.78±1.52 days), pupal period (9.41±0.70 days and 10.33±1.08 days) did not vary significantly between modified diet and natural host. Fecundity in refined artificial diet was 66.00 ± 4.00 and it was 65.99± 7.02 eggs on natural host plant. Per cent egg hatching was almost same in both the cases.


Oikos ◽  
2003 ◽  
Vol 100 (1) ◽  
pp. 172-184 ◽  
Author(s):  
Geraldine A. Wright ◽  
Stephen J. Simpson ◽  
David Raubenheimer ◽  
Philip C. Stevenson

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