How well do isolated lignins mimic the inhibitory behaviour of cell wall lignins during enzymatic hydrolysis of hydrothermally treated softwood?

Fruit bodies (separately pilei and stems) of mushrooms Pleurotus ostreatus (four strains) and Pleurotus eryngii were characterised as a source of polysaccharides. The contents of glucans and dietary fibres were determined with using the respective Megazyme enzymatic kits. Enzymatic analysis of the fruit bodies confirmed significant differences in the contents of these components among the species and strains. The stems contained more insoluble dietary fibres than the pilei in all the cases and more β-glucans in most cases. However, relatively high contents of β-glucan (20–50% of dry matter) could be a result of incomplete enzymatic hydrolysis of insoluble α-1,3-glucans. Nevertheless, low food quality stems of mushrooms Pleurotus sp. could be a valuable source of cell wall glucans for the preparation of food supplements.

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Synergetic Dissolution of Branched Xylan and Lignin Opens the Way for Enzymatic Hydrolysis of Poplar Cell Wall

Journal of Agricultural and Food Chemistry ◽

10.1021/acs.jafc.8b00320 ◽

2018 ◽

Vol 66 (13) ◽

pp. 3449-3456 ◽

Cited By ~ 7

Author(s):

Xia Zhou ◽

Dayong Ding ◽

Tingting You ◽

Xun Zhang ◽

Keiji Takabe ◽

...

Keyword(s):

Cell Wall ◽

Enzymatic Hydrolysis ◽

Hydrolysis Of ◽

The Way

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Diversity of Cell Wall Composition and Saccharides Production with H2SO4 Pretreatment in Bioenergy Material: Miscanthus

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.670-671.193 ◽

2014 ◽

Vol 670-671 ◽

pp. 193-196

Author(s):

Ning Xu ◽

Ben Lin Dai ◽

Zhen Wu ◽

Fei Hu Mu ◽

Ji Ming Xu

Keyword(s):

Cell Wall ◽

Enzymatic Hydrolysis ◽

Cell Wall Composition ◽

Bioenergy Crop ◽

Significant Difference ◽

Hydrolysis Of ◽

Supernatant Solution

Miscanthus is a high yielding bioenergy crop which has been tested as substrate for the bioprocess. In this study, we selected four typical pairs of Miscanthus samples which present different cell wall compositions. The sample shows significant difference in saccharine production with different concentration pretreatment of H2SO4(0.5%, 1.0%, and 4.0%). Much more pentose (about 15.0% -30.0%) was extracted in the supernatant solution; and no significant difference between four species. The hexose yield of all samples is disciplinary and not in order. Direct enzymatic hydrolysis of the H2SO4presoaked biomass was found to give higher hexose yields of 6.0%–13.0%, but low pentose yields of 1.5%–3.0%.Higher H2SO4concentration resulted in more hexose, but less pentose in enzymatic hydrolysis, while they exhibit good tendency in saccharin yields between four typical pairs.

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Compositional studies on the cell walls of the synnema and vegetative hyphae of Ceratocystis ulmi

Canadian Journal of Botany ◽

10.1139/b73-145 ◽

1973 ◽

Vol 51 (6) ◽

pp. 1147-1153 ◽

Cited By ~ 13

Author(s):

James L. Harris ◽

Willard A. Taber

Keyword(s):

Electron Microscopy ◽

Amino Acids ◽

Cell Wall ◽

Enzymatic Hydrolysis ◽

Cell Walls ◽

Amino Sugar ◽

Fibrillar Structure ◽

Dense Granules ◽

Hyphal Wall ◽

Hydrolysis Of

The composition of the cell walls of synnemal and vegetative hyphae of Ceratocystis ulmi was studied by fractionation and assay of released compounds. Residues after enzymatic hydrolyses were examined by electron microscopy. The synnemal wall was found to have 67% carbohydrate, 4.52% amino sugar, 5.02% protein, 1.6% lipid, and 0.59% ash, which accounted for 78.7% of the cell wall. The vegetative hyphal wall contained 56% carbohydrate, 3.44% amino sugar, 7.92% protein, 4.5% lipid, and 1.45% ash, which totaled 73.3% of the wall weight. Sugars identified were D-glucose, D-mannose, D-galactose, and L-rhamnose. Enzymatic hydrolysis of both wall types by cellulase and laminaranase indicated the presence of beta-1,3 and beta-1,4 linkages of glucose polymers. N-acetylglucosamine was liberated by chitinase. Most of the 16 amino acids detected in each wall type were at least twice as abundant in vegetative hyphal walls as in synnemal hyphal walls. Cellulase and laminaranase treatment of cell walls revealed a fibrillar structure. Chitinase-treated walls did not appear as fibrous, suggesting that the fibrous structure may be mostly chitinous. Synnemal cell walls are covered by electron-dense granules which may correspond to the pigment in the synnemal hyphae.

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Effect of storage of apple on the enzymatic hydrolysis of cell wall polysaccharides

Carbohydrate Polymers ◽

10.1016/s0144-8617(96)00043-4 ◽

1996 ◽

Vol 29 (4) ◽

pp. 301-307 ◽

Cited By ~ 14

Author(s):

P Massiot

Keyword(s):

Cell Wall ◽

Enzymatic Hydrolysis ◽

Cell Wall Polysaccharides ◽

Hydrolysis Of

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First result of tartaric stabilization by adding mannoproteins (Mannostab™)

OENO One ◽

10.20870/oeno-one.1997.31.1.1092 ◽

1997 ◽

Vol 31 (1) ◽

pp. 23 ◽

Cited By ~ 1

Author(s):

Virginie Moine-Ledoux ◽

A. Perrin ◽

I. Paladin ◽

Denis Dubourdieu

Keyword(s):

Cell Wall ◽

Enzymatic Hydrolysis ◽

New Product ◽

Model Solution ◽

The Other ◽

Inhibitor Activity ◽

Commercial Preparation ◽

Other Hand ◽

Crystallization Inhibitor ◽

Hydrolysis Of

Some mannoproteins which are extracted by enzymatic hydrolysis of yeast's cell wall with the aid of commercial preparation composed with beta-glucanases (Glucanex™) inhihits the tartaric acid's salt breakdown in model solution as well as in wines for an addition between 10 and 25 g/hl. On the other hand, heat extracted mannoproteins arc inactive on the tartaric precipitation stabilization. Furthermore, the crystallization inhibitor activity of the enzymatic extracted mannoproteins is due to the presence of sharply glycosylated mannoproteins of mediummolecular weight (30 to 50 kda) which are not heat extracted. That is why the heat extracted mannoproteins are inactive. The enzymatic extracted mannoproteins are produced at a pilot plan. This preparation (Mannostab™), soluble in wines, colourless and without any flavour, stabilize with the same efficiency than a freeze treatment and this effective live is superior to the metatartric acid. Moreover, the necessary steps were been taked to permit experimentally this new product : Mannostab™

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Effect of pretreatment and enzymatic hydrolysis of wheat straw on cell wall composition, hydrophobicity and cellulase adsorption

Bioresource Technology ◽

10.1016/j.biortech.2011.03.011 ◽

2011 ◽

Vol 102 (10) ◽

pp. 5938-5946 ◽

Cited By ~ 55

Author(s):

Senta Heiss-Blanquet ◽

Dan Zheng ◽

Nicolas Lopes Ferreira ◽

Catherine Lapierre ◽

Stéphanie Baumberger

Keyword(s):

Cell Wall ◽

Enzymatic Hydrolysis ◽

Wheat Straw ◽

Cell Wall Composition ◽

Cellulase Adsorption ◽

Hydrolysis Of

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Diversity of Cell Wall Composition and Saccharides Production with NaOH Pretreatment in Bioethanol Material: Miscanthus

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.644-650.4740 ◽

2014 ◽

Vol 644-650 ◽

pp. 4740-4743

Author(s):

Ning Xu ◽

Ben Lin Dai ◽

Zhen Wu ◽

Fei Hu Mu ◽

Ji Ming Xu

Keyword(s):

Cell Wall ◽

Enzymatic Hydrolysis ◽

Cell Wall Composition ◽

Bioenergy Crop ◽

Glucose Yield ◽

Naoh Pretreatment ◽

Significant Difference ◽

Hydrolysis Of ◽

Average Glucose ◽

Supernatant Solution

Miscanthus is a high yielding bioenergy crop. In this study, we selected four typical pairs of Miscanthus samples which presents different cell wall compositions. After different concentration pretreatment of NaOH (0.5%, 1.0%, 4.0%), four species shows significant difference in saccharine production. Much more xylose was extracted in the supernatant solution; the average glucose yield of all samples was about 2.0% in the concentration of 4.0%, which while the xylose yield was 12.0%. Direct enzymatic hydrolysis of the NaOH presoaked biomass was found to give higher glucose yields of 11–26%, and low xylose yields of 11–15%.Lower concentration of NaOH resulted in distinct differences in both glucose and xylose hydrolysis yields.

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