Diversity of Cell Wall Composition and Saccharides Production with NaOH Pretreatment in Bioethanol Material: Miscanthus

2014 ◽  
Vol 644-650 ◽  
pp. 4740-4743
Author(s):  
Ning Xu ◽  
Ben Lin Dai ◽  
Zhen Wu ◽  
Fei Hu Mu ◽  
Ji Ming Xu
Keyword(s):  
Cell Wall ◽  
Enzymatic Hydrolysis ◽  
Cell Wall Composition ◽  
Bioenergy Crop ◽  
Glucose Yield ◽  
Naoh Pretreatment ◽  
Significant Difference ◽  
Hydrolysis Of ◽  
Average Glucose ◽  
Supernatant Solution

Miscanthus is a high yielding bioenergy crop. In this study, we selected four typical pairs of Miscanthus samples which presents different cell wall compositions. After different concentration pretreatment of NaOH (0.5%, 1.0%, 4.0%), four species shows significant difference in saccharine production. Much more xylose was extracted in the supernatant solution; the average glucose yield of all samples was about 2.0% in the concentration of 4.0%, which while the xylose yield was 12.0%. Direct enzymatic hydrolysis of the NaOH presoaked biomass was found to give higher glucose yields of 11–26%, and low xylose yields of 11–15%.Lower concentration of NaOH resulted in distinct differences in both glucose and xylose hydrolysis yields.

Diversity of Cell Wall Composition and Saccharides Production with H2SO4 Pretreatment in Bioenergy Material: Miscanthus

2014 ◽  
Vol 670-671 ◽  
pp. 193-196
Author(s):  
Ning Xu ◽  
Ben Lin Dai ◽  
Zhen Wu ◽  
Fei Hu Mu ◽  
Ji Ming Xu
Keyword(s):  
Cell Wall ◽  
Enzymatic Hydrolysis ◽  
Cell Wall Composition ◽  
Bioenergy Crop ◽  
Significant Difference ◽  
Hydrolysis Of ◽  
Supernatant Solution

Miscanthus is a high yielding bioenergy crop which has been tested as substrate for the bioprocess. In this study, we selected four typical pairs of Miscanthus samples which present different cell wall compositions. The sample shows significant difference in saccharine production with different concentration pretreatment of H2SO4(0.5%, 1.0%, and 4.0%). Much more pentose (about 15.0% -30.0%) was extracted in the supernatant solution; and no significant difference between four species. The hexose yield of all samples is disciplinary and not in order. Direct enzymatic hydrolysis of the H2SO4presoaked biomass was found to give higher hexose yields of 6.0%–13.0%, but low pentose yields of 1.5%–3.0%.Higher H2SO4concentration resulted in more hexose, but less pentose in enzymatic hydrolysis, while they exhibit good tendency in saccharin yields between four typical pairs.

Effect of pretreatment and enzymatic hydrolysis of wheat straw on cell wall composition, hydrophobicity and cellulase adsorption

2011 ◽  
Vol 102 (10) ◽  
pp. 5938-5946 ◽  
Author(s):  
Senta Heiss-Blanquet ◽  
Dan Zheng ◽  
Nicolas Lopes Ferreira ◽  
Catherine Lapierre ◽  
Stéphanie Baumberger
Keyword(s):  
Cell Wall ◽  
Enzymatic Hydrolysis ◽  
Wheat Straw ◽  
Cell Wall Composition ◽  
Cellulase Adsorption ◽  
Hydrolysis Of

scholarly journals Study of the effect of process parameters on the yield of fermentable sugar from red cocoyam (Xanthosoma sagittifolium) peels via acid and enzyme hydrolysis

2021 ◽  
Vol 1 (1) ◽  
pp. 061-069
Author(s):  
Onoh Ikechukwu Maxwell ◽  
Anho Lawrence Oghenerivwe ◽  
Egwuagu Onyekachi
Keyword(s):  
Enzymatic Hydrolysis ◽  
Functional Groups ◽  
Process Parameters ◽  
Maximum Yield ◽  
Proximate Analysis ◽  
Enzyme Hydrolysis ◽  
Glucose Yield ◽  
Xanthosoma Sagittifolium ◽  
Hydrolysis Of ◽  
Fermentative Process

The aim of this work is to study the acid and enzymatic hydrolysis of cocoyam peels using HCl, H2S04 acids and cellulase enzyme. The cellulase was secreted from Aspergillus Niger (A. niger) fungi. The proximate analysis of the substrate showed that cocoyam peel is a lignocellulosic biomass with a cellulose composition of 48%. The effect of the process parameters (time, temperature, acid concentration and pH) on the yield of glucose in acid and enzymatic hydrolysis of the cocoyam peel was respectively investigated. Maximum glucose yield of 44.5% was obtained after 3 days of enzymatic hydrolysis at 30°C and pH 5. The HCl acid hydrolysis showed a maximum glucose yield of 27.3% at 70°C, 5% HCl after 180 minutes. The glucose yield in H2S04 hydrolysis was relatively lower than that of the HCl with a maximum yield of 26.5% at 70°C, 5% H2SO4 after 180 minutes. In addition to, the functional groups present in the glucose synthesized from cocoyam ground peels and the standard glucose were evaluated using Fourier Transformed Infrared (FTIR). The FTIR results showed similarities in the functional groups present in both sugars. Cocoyam peel can be used for the production of glucose and further fermentative process to produce ethanol.

scholarly journals Mushrooms of genus Pleurotus as a source of dietary fibres and glucans for food supplements

2009 ◽  
Vol 26 (No. 6) ◽  
pp. 441-446 ◽  
Author(s):  
A. Synytsya ◽  
K. Míčková ◽  
I. Jablonský ◽  
M. Sluková ◽  
J. Čopíková
Keyword(s):  
Cell Wall ◽  
Enzymatic Hydrolysis ◽  
Food Quality ◽  
Pleurotus Ostreatus ◽  
Dry Matter ◽  
Pleurotus Eryngii ◽  
Food Supplements ◽  
Enzymatic Analysis ◽  
Dietary Fibres ◽  
Hydrolysis Of

Fruit bodies (separately pilei and stems) of mushrooms <i>Pleurotus ostreatus</i> (four strains) and <i>Pleurotus eryngii</i> were characterised as a source of polysaccharides. The contents of glucans and dietary fibres were determined with using the respective Megazyme enzymatic kits. Enzymatic analysis of the fruit bodies confirmed significant differences in the contents of these components among the species and strains. The stems contained more insoluble dietary fibres than the pilei in all the cases and more β-glucans in most cases. However, relatively high contents of β-glucan (20–50% of dry matter) could be a result of incomplete enzymatic hydrolysis of insoluble α-1,3-glucans. Nevertheless, low food quality stems of mushrooms <i>Pleurotus</i> sp. could be a valuable source of cell wall glucans for the preparation of food supplements.

scholarly journals Saccharification Yield through Enzymatic Hydrolysis of the Steam-Exploded Pinewood

Energies ◽  
2020 ◽  
Vol 13 (17) ◽  
pp. 4552 ◽  
Author(s):  
Merve Nazli Borand ◽  
Asli Isler Kaya ◽  
Filiz Karaosmanoglu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Steam Explosion ◽  
Liquid Fraction ◽  
Sugar Content ◽  
Release Time ◽  
Variable Parameters ◽  
Glucose Yield ◽  
Steam Explosion Pretreatment ◽  
Saccharification Yield ◽  
Hydrolysis Of

Pressure, temperature, and retention time are the most studied parameters in steam explosion pretreatment. However, this work aimed to fix these parameters and to evaluate the influences of several less investigated steam explosion parameters on the saccharification yield in hydrolysis. In this study, firstly, pinewood samples smaller than 200 µm were treated with steam explosion at 190 °C for 10 min. The variable parameters were biomass loading, N2 pressure, and release time. Steam-exploded samples were hydrolyzed with the Trichoderma reesei enzyme for saccharification for 72 h. The sugar content of the resultant products was analyzed to estimate the yield of sugars (such as glucose, xylose, galactose, mannose, and arabinose). The best glucose yield in the pulp was achieved with 4 g of sample, N2 pressure of 0.44 MPa, and short release time (22 s). These conditions gave a glucose yield of 97.72% in the pulp, and the xylose, mannose, galactose, and arabinose yields in the liquid fraction were found to be 85.59%, 87.76%, 86.43%, and 90.3%, respectively.

Synergetic Dissolution of Branched Xylan and Lignin Opens the Way for Enzymatic Hydrolysis of Poplar Cell Wall

2018 ◽  
Vol 66 (13) ◽  
pp. 3449-3456 ◽  
Author(s):  
Xia Zhou ◽  
Dayong Ding ◽  
Tingting You ◽  
Xun Zhang ◽  
Keiji Takabe ◽  
...  
Keyword(s):  
Cell Wall ◽  
Enzymatic Hydrolysis ◽  
Hydrolysis Of ◽  
The Way
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