Co-infusion of insulin-secreting adipose tissue-derived mesenchymal stem cells and hematopoietic stem cells: novel approach to management of type 1 diabetes mellitus

2015 ◽  
Vol 36 (4) ◽  
pp. 426-432 ◽  
Author(s):  
U. G. Thakkar ◽  
H. L. Trivedi ◽  
A. V. Vanikar ◽  
S. D. Dave
Keyword(s):  
Diabetes Mellitus ◽  
Stem Cells ◽  
Type 1 Diabetes ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Hematopoietic Stem Cells ◽  
Type 1 Diabetes Mellitus ◽  
Hematopoietic Stem ◽  
Novel Approach

Alleviation of exogenous insulin requirement in type 1 diabetes mellitus after immunoablation and transplantation of autologous hematopoietic stem cells

2009 ◽  
Vol 119 (6) ◽  
pp. 422-426
Author(s):  
Emilian Snarski ◽  
Tigran Torosian ◽  
Monika Paluszewska ◽  
Elżbieta Urbanowska ◽  
Alicja Milczarczyk ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Stem Cells ◽  
Type 1 Diabetes ◽  
Hematopoietic Stem Cells ◽  
Type 1 Diabetes Mellitus ◽  
Insulin Requirement ◽  
Exogenous Insulin ◽  
Hematopoietic Stem

Mesenchymal Stem Cells in the Treatment of Type 1 Diabetes Mellitus

2015 ◽  
Vol 26 (2) ◽  
pp. 95-103 ◽  
Author(s):  
Jana Katuchova ◽  
Denisa Harvanova ◽  
Timea Spakova ◽  
Rastislav Kalanin ◽  
Daniel Farkas ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Stem Cells ◽  
Type 1 Diabetes ◽  
Mesenchymal Stem Cells ◽  
Type 1 Diabetes Mellitus

The effect of exosomes derived from mesenchymal stem cells in the treatment of induced type 1 diabetes mellitus in rats

2020 ◽  
Vol 42 (8) ◽  
pp. 1597-1610 ◽  
Author(s):  
Dina Sabry ◽  
Samar Marzouk ◽  
Reem Zakaria ◽  
Heba A. Ibrahim ◽  
Mai Samir
Keyword(s):  
Diabetes Mellitus ◽  
Stem Cells ◽  
Type 1 Diabetes ◽  
Mesenchymal Stem Cells ◽  
Type 1 Diabetes Mellitus

scholarly journals Wip1 regulates the immunomodulatory effects of murine mesenchymal stem cells in type 1 diabetes mellitus via targeting IFN-α/BST2

2020 ◽  
Author(s):  
Na Zhou ◽  
Weijiang Liu ◽  
Yuanlin Liu ◽  
Xue Li ◽  
Yang Wang ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Stem Cells ◽  
Type 1 Diabetes ◽  
Mesenchymal Stem Cells ◽  
Type 1 Diabetes Mellitus ◽  
Pancreatic Tissue ◽  
Enzyme Linked Immunosorbent Assay ◽  
Therapeutic Effects ◽  
Wild Type

Abstract Background: Mesenchymal stem cells (MSCs) shows significant therapeutic effects in type 1 diabetes mellitus (T1DM) as they could regulate the inflammatory processes. However, little is known about the process of MSCs immunosuppression in T1DM. In this study, we investigated the effects of wild type p53-induce phosphatase 1 (Wip1) on regulating MSCs immunosuppressive capacities in T1DM mice.Methods: Primary wild type (Wip1+/+) MSCs and Wip1 knockout (Wip1−/−) MSCs were cultured in vitro. T1DM mouse model was induced with streptozotocin and then was treated with Wip1+/+ MSCs (5 × 105) or Wip1−/− MSCs (5 × 105) by tail vein injection. The general physiological states of T1DM mice were measured every week. Moreover, the pathological changes in the pancreatic tissue were observed. Enzyme-linked immunosorbent assay (ELISA) and flow cytometry were used to detect the expressions of inflammatory cytokines in mice.Results: Wip1 −/− MSCs had lower therapeutic effects in T1DM mice. Moreover, we screened and confirmed bone marrow stromal cell antigen2 (BST2) gene that showed the target gene for Wip1 through gene chips, quantitative polymerase chain reaction and Western blot. Wip1−/− MSCs exhibited lower immunosuppressive capacity, as evidenced by enhanced expression of BST2, with concurrent increased expression of interferon-α (IFN-α). In vivo distribution analysis results indicated that Wip1−/− MSCs homed to the damaged pancreatic tissue. Wip1−/− MSCs influenced the expression of immune factors by remarkably increasing the expression of tumor necrosis factor-α (TNF-α), interleukin-17A (IL-17A), IFN-α, IFN-β, and IFN-γ and decreasing the expression of IL-4 and IL-10.Conclusions: Wip1 affects MSCs immunomodulation by regulating the expression of IFN-α/BST2. These findings suggest that Wip1 is required to regulate the therapeutic effects of MSCs on T1DM treatment, indicating a novel role of Wip1 in immunoregulation.

scholarly journals Exosome Degeneration in Mesenchymal Stem Cells Derived from Patients with Type 1 Diabetes Mellitus

2021 ◽  
Vol 22 (20) ◽  
pp. 10906
Author(s):  
Michiko Horiguchi ◽  
Yuko Okada ◽  
Yuya Turudome ◽  
Kentaro Ushijima
Keyword(s):  
Diabetes Mellitus ◽  
Stem Cells ◽  
Type 1 Diabetes ◽  
Mesenchymal Stem Cells ◽  
Type 1 Diabetes Mellitus ◽  
Adipose Derived Stem Cells ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
Healthy Humans

Type 1 diabetes mellitus is characterized by the destruction of pancreatic β-cells and requires the regeneration of these destroyed pancreatic β-cells for radical treatment. The degeneration of organelles in stem cells compromises stem cell quality; however, organelles in the mesenchymal stem cells of patients with type 1 diabetes mellitus have not been characterized previously. In this study, we use transmission electron microscopy to evaluate the degeneration of organelles in adipose-derived stem cells of patients with type 1 diabetes mellitus (T1DM ADSCs). Compared to adipose-derived stem cells from healthy humans, T1DM ADSCs degenerate differently, characterized by prominent enlarged spherical vesicles. The exosomes of T1DM ADSCs are found to be enlarged, reduced in number, and increased in the percentage of those positive for tetraspanin CD9. The findings of this study provide insight into the characteristics of stem cells in patients with type 1 diabetes mellitus.

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