Antimicrobial activity of green silver nanoparticles produced using aqueous leaf extract of Hydrocotyle rotundifolia

2016 ◽  
Vol 16 (3) ◽  
pp. 195-201 ◽  
Author(s):  
Ragini Kumari ◽  
Girija Brahma ◽  
Sumit Rajak ◽  
Mukesh Singh ◽  
Santosh Kumar
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Aqueous Leaf Extract

Green Synthesis of Silver Nanoparticles by Allamanda cathartica L. Leaf Extract and Evaluation for Antimicrobial Activity

2013 ◽  
Vol 6 (4) ◽  
pp. 2260-2268
Author(s):  
M. Linga Rao ◽  
Bhumi G ◽  
Savithramma N
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Fusarium Species ◽  
Inhibition Zone ◽  
Fungal Species ◽  
Green Technology ◽  
Metallic Silver ◽  
Synthesis Of Nanoparticles ◽  
Aqueous Leaf Extract

Silver nanoparticles (SNPs) exhibit tremendous applications in medicine as antimicrobial agent.  The use of different parts of plants for the synthesis of nanoparticles is considered as a green technology as it does not involve any harmful chemicals.  In the present study, we report a rapid biosynthesis of silver nanoparticles from aqueous leaf extract of medicinal plant Allamanda cathartica.  The active phytochemicals present in the plant were responsible for the quick reduction of silver ion to metallic silver nanoparticles. The reduced silver nanoparticles were characterized by using UV-Vis spectrophotometry, Scanning Electron Microscope (SEM), Energy Dispersive Analysis of X-ray (EDAX) and Atomic Force Microscopy (AFM).  The spherical shaped silver nanoparticles were observed and it was found to 19-40 nm range of size.  These phytosynthesized SNPs were tested for their antimicrobial activity and it analyzed by measuring the inhibitory zone. A. cathartica aqueous leaf extract of SNPs showed highest toxicity to Pseudomonas followed by Klebsiella, Bacillus and E. coli and lowest toxicity towards Proteus. In fungal species, highest inhibition zone was noted against Rhizopus followed by Curvularia, Aspergillus flavus and Aspergillus niger and minimum inhibition zone was observed against Fusarium species.  These results suggest a promising potential of Indian plant-based green chemistry for production of SNPs for biomedical and nanotechnology applications.

Green Synthesis of Silver Nanoparticles from Aqueous Leaf Extract of 'Selaginella bryopteris' and Elucidation of its Antimicrobial Activity

2020 ◽  
Vol 16 (4) ◽  
pp. 449-459
Author(s):  
Varsha Yadav ◽  
Neha Kapoor ◽  
Soma M. Ghorai ◽  
Pradeep
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Aqueous Leaf Extract ◽  
Selaginella Bryopteris

Background: Biosynthesis of nanoparticles from aqueous leaf extract of ‘Selaginella bryopteris’ is a green chemistry approach and is considered to be one of the most efficient methods as it is devoid of toxic chemicals as well as provides natural capping agents for the stabilization of synthesized nanoparticles. ‘S.bryopteris’ also known as ‘Sanjeevani’ (in India), is thought to be prospective natural resource that possesses extraordinary pharmaceutical potential. Objective: S. bryopteris is exclusively native to India and has already been known for its expression of stress-associated genes and high levels of protective metabolites of sugars, phenolic compounds, and polyols. Its potential as an antibacterial agent is being elucidated. Methods: Different leaf extract volumes, silver nitrate (AgNO3) concentrations, and reaction time were investigated separately and the optimal conditions for the synthesis of AgNPs were suggested. The resulting AgNPs were characterized by various techniques like Ultraviolet-Visible (UV-Vis) Spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM) and XRay Diffraction (XRD). Antibacterial assays were carried out by using both agar disk and well diffusion method. Results: The AgNPs synthesized in this process were found to have efficient antimicrobial activity against both Gram-positive as well as Gram-negative bacteria. The antibacterial efficacy of S. bryopteris was consciously tried on uropathogenic Escherichia coli (Gram-negative bacteria) and Bacillus megaterium (Gram-positive bacteria) which have the self-limiting food poisoning potential along with opportunistic uropathogenic bacterial strains namely Proteus mirabilis (Gram-negative bacteria) and a non-pathogenic Micrococcus luteus (Gram-positive bacteria) for comparison. Conclusion: S. bryopteris mediated silver nanoparticles’ synthesis is attempted for being cost-effective, eco-friendly and safe for human therapeutics.

scholarly journals Biogenic synthesis, characterization and antimicrobial activity of Ixora brachypoda (DC) leaf extract mediated silver nanoparticles

2020 ◽  
pp. 101296
Author(s):  
Meghashyama Bhat ◽  
Bidhayak Chakraborty ◽  
Raju Suresh Kumar ◽  
Abdulrahman I. Almansour ◽  
Natarajan Arumugam ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Biogenic Synthesis

A dual-purpose silver nanoparticles biosynthesized using aqueous leaf extract of Detarium microcarpum : An under-utilized species

Talanta ◽  
2016 ◽  
Vol 160 ◽  
pp. 735-744 ◽  
Author(s):  
Ayomide H. Labulo ◽  
Elijah T. Adesuji ◽  
Oyinade A. Dedeke ◽  
Olusola S. Bodede ◽  
Charles O. Oseghale ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Dual Purpose ◽  
Detarium Microcarpum ◽  
Aqueous Leaf Extract

Biosynthesis of silver nanoparticles using Ocimum sanctum (Tulsi) leaf extract and screening its antimicrobial activity

2011 ◽  
Vol 13 (7) ◽  
pp. 2981-2988 ◽  
Author(s):  
Garima Singhal ◽  
Riju Bhavesh ◽  
Kunal Kasariya ◽  
Ashish Ranjan Sharma ◽  
Rajendra Pal Singh
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Ocimum Sanctum

Synthesis and Characterization of Silver Nanoparticles from Couroupita guianensis leaf extract–A Simple Green Route

2021 ◽  
pp. 2796-2800
Author(s):  
Inbathamizh L ◽  
Kalpana V ◽  
Revathi Yadav K
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Nitrate Solution ◽  
Ultra Violet ◽  
Silver Nitrate Solution ◽  
Green Route ◽  
Couroupita Guianensis ◽  
Potential Applications ◽  
Aqueous Leaf Extract

With the increase in the potential applications of nanoparticles in pharma and various fields, nanoparticle research is attracting more attention. Though several chemical and physical methods are being used for the synthesis of metal nanoparticles, they are associated with several disadvantages. Couroupita guianensis is a traditional plant with medicinal values. The focus of the study was to follow a green chemistry route to synthesize silver nanoparticles (AgNPs) using the leaf extract of Couroupita guianensis as a reductant and stabilizing agent. The boiled aqueous leaf extract with silver nitrate solution on exposure to sunlight showed the maximum absorbance at 430nm indicating the synthesis of AgNPs. Ultra Violet (UV)-Visible spectroscopy, Scanning Electron Microscopy (SEM), and Fourier Transform Infrared spectroscopy (FTIR) techniques were used for the characterization of AgNPs. The synthesized AgNPs were found to be spherical and 4.44 – 40.20nm in size. They also seemed to be capped with the significant functional groups present in the leaf extract. Thus, the study suggested Couroupita guianensis mediated green synthesis of AgNPs as an efficient and eco-friendly approach with substantial advantages over the conventional methods. The process could be further scaled-up for mass production and wider applications of AgNPs.

scholarly journals Biogenic silver nanoparticles from Trichodesma indicum aqueous leaf extract against Mythimna separata and evaluation of its larvicidal efficacy

2017 ◽  
Vol 57 (2) ◽  
pp. 194-200 ◽  
Author(s):  
Abdul A. Buhroo ◽  
Gousul Nisa ◽  
Syed Asrafuzzaman ◽  
Ram Prasad ◽  
Razia Rasheed ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Average Particle ◽  
Particle Sizes ◽  
Mythimna Separata ◽  
Ag Nps ◽  
Leaf Extracts ◽  
Tem Analysis ◽  
Vis Spectroscopy ◽  
Aqueous Leaf Extract

AbstractThe present exploration is focused on the bio-fabrication of silver nanoparticles (Ag NPs) usingTrichodesma indicumaqueous leaf extract as a reducing agent. The synthesized Ag NPs were productively characterized by UV-vis spectroscopy, XRD, and TEM studies. The photosynthesis of Ag NPs was done at room temperature for 24 h and at 60°C. The green synthesis of spherical-shaped Ag NPs bio-fabricated fromT. indicumwith a face centred cubic structure showed average particle sizes of 20–50 nm, which is inconsistent with the particle size calculated by the XRD Scherer equation and TEM analysis. We further explored the larvicidal efficacy of biosynthesized Ag NPs with leaf extracts ofT. indicumagainstMythimna separata. The results showed that Ag NPs (20–50 nm) ofT. indicumpossess good larvicidal activity againstM. separatawith an LC50of 500 ppm. Thus, we can advocate that Ag NPs of 20–50 nm size extracted fromT. indicummay be considered in the pest management programme ofM. separatain future.

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