Synthesis and Characterization of Silver Nanoparticles from Couroupita guianensis leaf extract–A Simple Green Route

2021 ◽  
pp. 2796-2800
Author(s):  
Inbathamizh L ◽  
Kalpana V ◽  
Revathi Yadav K
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Nitrate Solution ◽  
Ultra Violet ◽  
Silver Nitrate Solution ◽  
Green Route ◽  
Couroupita Guianensis ◽  
Potential Applications ◽  
Aqueous Leaf Extract

With the increase in the potential applications of nanoparticles in pharma and various fields, nanoparticle research is attracting more attention. Though several chemical and physical methods are being used for the synthesis of metal nanoparticles, they are associated with several disadvantages. Couroupita guianensis is a traditional plant with medicinal values. The focus of the study was to follow a green chemistry route to synthesize silver nanoparticles (AgNPs) using the leaf extract of Couroupita guianensis as a reductant and stabilizing agent. The boiled aqueous leaf extract with silver nitrate solution on exposure to sunlight showed the maximum absorbance at 430nm indicating the synthesis of AgNPs. Ultra Violet (UV)-Visible spectroscopy, Scanning Electron Microscopy (SEM), and Fourier Transform Infrared spectroscopy (FTIR) techniques were used for the characterization of AgNPs. The synthesized AgNPs were found to be spherical and 4.44 – 40.20nm in size. They also seemed to be capped with the significant functional groups present in the leaf extract. Thus, the study suggested Couroupita guianensis mediated green synthesis of AgNPs as an efficient and eco-friendly approach with substantial advantages over the conventional methods. The process could be further scaled-up for mass production and wider applications of AgNPs.

scholarly journals Degradation of Methylene Blue Using Biologically Synthesized Silver Nanoparticles

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
M. Vanaja ◽  
K. Paulkumar ◽  
M. Baburaja ◽  
S. Rajeshkumar ◽  
G. Gnanajobitha ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Nitrate Solution ◽  
Reaction Medium ◽  
Silver Ions ◽  
Silver Nitrate Solution ◽  
X Ray ◽  
Synthesis Of Nanoparticles ◽  
Aqueous Leaf Extract

Nowadays plant mediated synthesis of nanoparticles has great interest and achievement due to its eco-benign and low time consuming properties. In this study silver nanoparticles were successfully synthesized by usingMorinda tinctorialeaf extract under different pH. The aqueous leaf extract was added to silver nitrate solution; the color of the reaction medium was changed from pale yellow to brown and that indicates reduction of silver ions to silver nanoparticles. Thus synthesized silver nanoparticles were characterized by UV-Vis spectrophotometer. Dispersity and morphology was characterized by scanning electron microscope (SEM); crystalline nature and purity of synthesized silver nanoparticles were revealed by X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDX). FTIR spectrum was examined to identify the effective functional molecules responsible for the reduction and stabilization of silver nanoparticles synthesized by leaf extract. The photocatalytic activity of the synthesized silver nanoparticles was examined by degradation of methylene blue under sunlight irradiation. Green synthesized silver nanoparticles were effectively degrading the dye nearly 95% at 72 h of exposure time.

Controlling the Surface Plasmon Absorption of Silver Nanoparticles via Green Synthesis Using Pennisetum purpureum Leaf Extract

2018 ◽  
Vol 772 ◽  
pp. 73-77
Author(s):  
Ruelson S. Solidum ◽  
Arnold C. Alguno ◽  
Rey Capangpangan
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Silver Nitrate ◽  
Surface Plasmon ◽  
Leaf Extract ◽  
Nitrate Solution ◽  
Silver Nitrate Solution ◽  
Absorption Peak ◽  
Plasmon Absorption ◽  
Surface Plasmon Absorption

We report on the green synthesis of silver nanoparticles utilizing theP.purpureumleaf extract. Controlling the surface plasmon absorption of silver nanoparticles was achieved by regulating the amount of extract concentration and the molarity of silver nitrate solution. The surface plasmon absorption peak is found at around 430nm. The surface plasmon absorption peak have shifted to lower wavelength as the amount of extract is increased, while plasmon absorption peak shifts on a higher wavelength as the concentration of silver nitrate is increased before it stabilized at 430nm. This can be explained in terms of the available nucleation sites promoted by the plant extract as well as the available silver ions present in silver nitrate solution.

scholarly journals Synthesis, characterization and antibacterial potential of silver nanoparticles by Morus nigra leaf extract

2013 ◽  
Vol 1 (04) ◽  
pp. 16-24 ◽  
Author(s):  
Anu Kumar ◽  
Kuldeep Kaur ◽  
Sarika Sharma
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Bacterial Species ◽  
Nitrate Solution ◽  
Average Particle Size ◽  
Silver Nitrate Solution ◽  
Sem Analysis ◽  
Average Particle ◽  
Morus Nigra ◽  
Uv Visible

The present study reports the synthesis of silver nanoparticle using Morus nigra leaf extract were used as reducing agent for reduction of silver nitrate solution. The synthesis of silver nanoparticles was analyzed by UV-Visible spectroscopy, Scanning Electron Microscopy. The SEM analysis has shown that size of silver nanoparticles synthesized from leaves extract of M.nigra was 200 nm and seems to be spherical in morphology. Morphology of chemically synthesized silver nanoparticles is nearly spherical and of size ranges from 300-500 nm. The average particle size analyzed from SEM analysis was observed to be 350 nm. This article has discussed the synthesis of silver nanoparticles generated from plant extract, characterization and antibacterial analysis. In this study the antibacterial activity was examined against six MTCC cultures collected from IMTECH Chandigarh, Including both gram positive and gram negative bacteria such as P.aeruginosa, S.aureus, B.subtilis, E.coli, P.flourescens and Streptococus mutans. Out of these strains the antimicrobial activity of the silver nanoparticles showed maximum zone of inbhition against P.flourescens (22 mm), P.aeruginosa (19 mm), S.aureus (18 mm) and least effective against E.coli (15mm). In contrast chemically synthesized silver nanoparticles were found most effective against S.aureus (13 mm) and B.subtilis (12mm) and almost ineffective against Streptococcus mutans (6 mm) and P.flourescens (4 mm). In the concluding remarks, the silver nanoparticles synthesized using M.nigra leaves extract would be a better antimicrobial effective against various bacterial species.

scholarly journals Evaluation of the Cytotoxic Activity of Biosynthesized Silver Nanoparticles Using Berberis vulgaris Leaf Extract

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Akbar Safipour Afshar ◽  
Fatemeh Saeid Nematpour
Keyword(s):  
Silver Nanoparticles ◽  
Cytotoxic Activity ◽  
Leaf Extract ◽  
Nitrate Solution ◽  
Silver Nitrate Solution ◽  
X Ray Diffraction ◽  
Berberis Vulgaris ◽  
Transmission Electron ◽  
Cancerous Cells ◽  
Mcf 7

Background: Recently, due to the numerous applications of silver nanoparticles (AgNPs) in industry, various routes to synthesize them have been developed. Objectives: The current study was aimed at synthesizing silver nanoparticles by the leaf extract of Berberis vulgaris and evaluating the cytotoxic effects on human breast cancer MCF-7 cell line. Methods: The leaf extract of Berberis vulgaris and silver nitrate solution were used to synthesize silver nanoparticles. Ultraviolet (UV)-visible, Fourier-transform infrared, and X-ray diffraction analysis spectroscopy and transmission electron microscopy methods were used to characterize and confirm the nanoparticles’ synthesis. The cytotoxic activity of synthesized nanoparticles (0, 5,10, 20, 40 µg/mL) was also studied by MTT assay. Results: The results showed that Ag nanoparticles were polydisperse and spherical in shape and had a size of about 19.9 nm. Silver nanoparticles reduced the growth of cancerous cells based on time and concentration. The IC50 for MCF-7 cells at 48 hours was 20.27 µg/mL. Conclusions: The findings showed that synthesized nanoparticles have an appropriate cytotoxic effect on cancer cells. This impact may be due to the production of free radicals through the release of Ag ions.

Rapid colorimetric detection of Hg2+ ion by green silver nanoparticles synthesized using Dahlia pinnata leaf extract

2015 ◽  
Vol 4 (6) ◽  
Author(s):  
Kaushik Roy ◽  
Chandan K. Sarkar ◽  
Chandan K. Ghosh
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Silver Nitrate ◽  
Leaf Extract ◽  
Colorimetric Detection ◽  
Nitrate Solution ◽  
Silver Nitrate Solution ◽  
First Time

AbstractIn this study, we for the first time reported green synthesis of silver nanoparticles from silver nitrate solution using leaf extract of

scholarly journals Green synthesis and characterization of silver nanoparticles using Indigofera aspalathoides leaf extract and study of its Antibacterial, Antioxidant, and Anti-cancerous activities

2021 ◽  
Vol 26 (3) ◽  
pp. 2606-2617
Author(s):  
V GANDHIRAJ ◽  
◽  
KUMAR K SATHISH ◽  
Keyword(s):  
Silver Nanoparticles ◽  
Silver Nitrate ◽  
Silver Nanoparticle ◽  
Leaf Extract ◽  
Nitrate Solution ◽  
Radical Scavenging ◽  
Silver Nitrate Solution ◽  
Bacterial Strains ◽  
Nanoparticle Formation ◽  
Average Grain Size

Indigofera aspalathoides is an indigenous medicinal plant with copious phytochemical and pharmacological benefits. The crude extract from the leaves of Indigofera apalathoides aids in the bioreduction of silver nitrate into silver ions which results in the formation of colloidal silver nanoparticles. Critical physio-chemical parameters such as temperature, pH, substrate ratio (ratio of leaf extract and silver nitrate solution) and incubation time were optimized to increase the yield and efficiency of the nanoparticle formation. Synthesized nanoparticles were then characterized by performing a set of spectral analyses such as UV, FTIR, XRD, SEM, EDAX and AFM, later its bioactivity was also assessed. Characteristic UV-Vis absorption peak (λmax) at the range of 360-390 nm indicated silver nanoparticle formation and XRD pattern revealed the crystalline nature, while the average grain size was estimated to be 68.5 nm using Scherrer’s formula. SEM image clearly depicted the square shaped silver nanoparticles, which were synthesized using Indigofera aspalathoides leaf extract. Confirmation of formation of silver nanoparticles were carried out using EDAX spectrum. IC 50 value of free radical scavenging occurred at a lower concentration of 300 µg/ml, while a maximum inhibition zone of 13 mm was recorded against pathogenic bacterial strains and 6.7 mm was recorded against pathogenic fungal strains. Further, the silver nanoparticle cytotoxicity analysis was also carried out using MCF 7 cell line and IC 50 was found out to be 5.9 µg/ml. Silver nanoparticles which are green synthesized using the aqueous extract of Indigofera aspalathoides proved as an effective broad-spectrum antibiotic as well as an efficient antioxidant and an anti-cancerous agent.

scholarly journals Corylus avellana leaf extract-mediated green synthesis of antifungal silver nanoparticles using microwave irradiation and assessment of their properties

2021 ◽  
Vol 10 (1) ◽  
pp. 606-613
Author(s):  
Mahsa Eshghi ◽  
Asa Kamali-Shojaei ◽  
Hamideh Vaghari ◽  
Yahya Najian ◽  
Zahra Mohebian ◽  
...  
Keyword(s):  
Particle Size ◽  
Silver Nanoparticles ◽  
Microwave Irradiation ◽  
Silver Nitrate ◽  
Leaf Extract ◽  
Nitrate Solution ◽  
Silver Nitrate Solution ◽  
Corylus Avellana ◽  
Transmission Electron ◽  
Minimum Particle Size

Abstract Due to high antimicrobial activity against numerous microorganisms, silver nanoparticles (AgNPs) are being utilized in various areas. Microwave-accelerated AgNPs synthesis using Corylus avellana leaf extract was evaluated. Based on randomly central composite design, 13 mixture solutions containing different amounts of the prepared extract (0.10–0.90 mL) and 1 mM silver nitrate solution (15–25 mL) were prepared and exposed to microwave irradiation for 180 s. Response surface methodology was utilized to evaluate the effects of the two independent variables on particle size and concentration of the synthesized AgNPs, as manifested in the place of broad emission peak (λ max) and its absorbance unit, respectively. Fourier transform infrared spectroscopy analysis indicated that the two hydroxyl and carboxylic acid functional groups with reducing activity existed in the prepared extract. Dynamic light scattering and transmission electron microscopy analyses revealed that the formed spherical AgNPs using optimum amounts of C. avellana leaf extract (0.9 mL) and 1 mM silver nitrate solution (25 mL) had minimum particle size (103.5 nm) and polydispersity index (PDI) (0.209), and maximum concentration (140 ppm) and zeta potential (−21.8 mV). Results indicated that the formed AgNPs had high fungicidal effects against the spoiled fungi of Colletotrichum coccodes and Penicillium digitatum.

scholarly journals Geranium wallichianum Leaf Extract Mediated Synthesis of Silver Nanoparticles: Characterization and its Antimicrobial Activity

2019 ◽  
Vol 31 (5) ◽  
pp. 1128-1132
Author(s):  
P.P. Badoni ◽  
Goutam Kumar ◽  
Mahender Singh ◽  
Navneet Singh ◽  
Arun K. Khajuria ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Antimicrobial Agents ◽  
Size Range ◽  
Nitrate Solution ◽  
Silver Nitrate Solution ◽  
Nano Particles ◽  
Diffusion Method ◽  
Cubic Phase ◽  
Absorption Bands

In present study, the Geranium wallichianum leaf extract (aqueous) and 1 mM silver nitrate solution (aqueous) were amalgamated to synthesize silver particles within the nanometer range. Initial colour changes and surface-plasmon-resonance (SPR) absorbance band observed in UV-visible spectroscopic study gave support to configuration of silver nano-particles. Silver nanoparticles were characterized by XRD, TEM and FTIR spectroscopy. Then antimicrobial (antibacterial and antifungal) activities of silver nanoparticles against selected microbial strains were studied by well diffusion method. During this work, silver SPR absorption bands between 405-425 nm range were shown by Geranium wallichianum leaf extract mediated synthesized silver nanoparticles sample solutions at different intervals. X-ray diffraction pattern displayed the formation of face centred cubic phase silver nano-structures. TEM measurements confirmed that silver nanoparticles are spherically shaped and maximum particles in 9-16 nm size range having average diameter 12.5 nm. Important FT-IR peaks at 3314.11, 1710.60, 1587.11 and 1347.77 cm-1 were predicted for hydroxyl, carbonyls, unsaturated C-C bonds and phenolic groups respectively. Further from antimicrobial results, it has been found that values of diameter of zone of inhibition (mm) of synthesized silver nanoparticles against B. subtilis, S. aureus, L. plantarum, P. aeruginosa, A. niger and C. albicans strains were 32, 28, 25, 25 35 and not active, respectively. MIC-MLC (μL) values were also determined. At last, it can be concluded that antimicrobial agents of 9-16 nm size range and stabilized by polyhydroxy-bioactive-components present in extract, in the form of silver nanoparticles has been productively synthesized.

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