Phytochemical analysis and antimicrobial potential of Nigrospora sphaerica (Berk. & Broome) Petch, a fungal endophyte isolated from Dillenia indica L.

2021 ◽  
Author(s):  
Vijay Kumar ◽  
I. B. Prasher
Keyword(s):  
Fungal Endophyte ◽  
Phytochemical Analysis ◽  
Antimicrobial Potential ◽  
Dillenia Indica

Phytochemical, In vitro Anti-inflammatory and Antimicrobial Potential of Hugonia mystax L.

2021 ◽  
pp. 169-173
Author(s):  
K.P. Jaiganesh ◽  
T.J. Jasna ◽  
A.C. Tangavelou
Keyword(s):  
Tamil Nadu ◽  
Diclofenac Sodium ◽  
Inflammatory Activity ◽  
Phytochemical Analysis ◽  
Membrane Stabilization ◽  
Traditional System ◽  
Antimicrobial Potential ◽  
Biochemical Compounds ◽  
Anti Inflammatory

Hugonia mystax L., (Linaceae), is commonly distributed in the thorny scrubs and tropical dry evergreen forests of Tamil Nadu, which has been valued for centuries in traditional system of medicine for the treatment of various ailments. In the present study was an attempt to investigate the phytochemical nature and anti-inflammatory, antimicrobial potential by adopting suitable methods. Phytochemical analysis of Hugonia mystax L., plant extracts revealed the presence of various biochemical compounds such as alkaloids, flavonoids, glycosides, triterpenoids and saponins etc. Since triterpenoids and flavonoids have remarkable anti-inflammatory activity, so our present work aims at evaluating in vitro anti inflammatory activity of Hugonia mystax L., by HRBC membrane stabilization method. The inhibition of hypotonicity induced HRBC membrane lysis was taken as a measure of the anti-inflammatory activity. The percentage of membrane stabilization for ethanolic extracts and Diclofenac sodium were done at different concentrations. The maximum membrane stabilization of Hugonia mystax L., extracts was found to be 94.97 % at a dose of 2000 μg/ml. Therefore, our studies support the isolation and the use of active constituents from Hugonia mystax L., in treating inflammations.

scholarly journals Lilium Philadelphicum Flower as a Novel Source of Antimicrobial Agents: A Study of Bioactivity, Phytochemical Analysis and Partial Identification of Antimicrobial Metabolites

2021 ◽  
Author(s):  
Shefali Singh ◽  
Vineeta Singh ◽  
Alaa Alhazami ◽  
B.N. Mishra ◽  
Shafiul Haque ◽  
...  
Keyword(s):  
Cell Viability ◽  
Bioactive Compounds ◽  
Bioactive Compound ◽  
Biologically Active ◽  
Gas Chromatography Mass Spectrometry ◽  
Achromobacter Xylosoxidans ◽  
Phytochemical Analysis ◽  
Antimicrobial Potential ◽  
Compound Concentration

The members of the Liliaceae family have been regarded as an excellent source of biologically active compounds. However, the work on antimicrobial potential and characterization of the bioactive fractions of Lilium philadelphicum flower is limited and needs to be explored. The present study reports the antimicrobial potential, anti-inflammatory and anticancer potential of the bioactive fraction extracted from the flower of L. philadelphicum (Red Lily) and characterization of these bioactive compounds. The antimicrobial activity was tested against nine different Gram-positive and Gram-negative bacterial strains. The minimum inhibitory concentration (MIC) values of methanolic extract of L. philadelphicum flower against Acinetobacter bouvetii, Achromobacter xylosoxidans, Bacillus subtilis MTCC 121, Candida albicans MTCC 183, Klebsiella pneumoniae MTCC 3384, and Salmonella typhi MTCC 537 were 25, 50, 12.5, 50, 100 and 50 μg mL-1, respectively. The phytochemical analysis of the extract reveals the presence of phenols, flavonoids, tannins, terpenoids, glycosides, coumarins, and quinones. The cytotoxicity of the partially purified compound against the HepG2 cell line in MTT assay demonstrates up to 90% cell viability with a bioactive compound concentration of 50 μg/ml. However, with the increase in bioactive compound concentration up to 1000 μg/ml results into nearly 80% cell viability, just a minor decline in cell viability suggests the importance of bioactive compounds for suitable therapeutic applications. Spectroscopic studies of the bioactive compound by UV-Visible spectroscopy, FT-Infra Red spectroscopy, Gas Chromatography-Mass Spectrometry (GCMS) as well as its phytochemical analysis suggests the presence of terpenoids moiety, responsible for the antimicrobial property of L. philadelphicum flower.

scholarly journals Lilium philadelphicum Flower as a Novel Source of Antimicrobial Agents: A Study of Bioactivity, Phytochemical Analysis, and Partial Identification of Antimicrobial Metabolites

2021 ◽  
Vol 13 (15) ◽  
pp. 8471
Author(s):  
Shefali Singh ◽  
Vineeta Singh ◽  
Alaa Alhazmi ◽  
Bhartendu Nath Mishra ◽  
Shafiul Haque ◽  
...  
Keyword(s):  
Cell Viability ◽  
Antimicrobial Agents ◽  
Antimicrobial Property ◽  
Bioactive Compound ◽  
Biologically Active ◽  
Gas Chromatography Mass Spectrometry ◽  
Achromobacter Xylosoxidans ◽  
Phytochemical Analysis ◽  
Antimicrobial Potential

The members of the Liliaceae family are considered an excellent source of biologically active compounds. However, work on antimicrobial potential and characterization of the bioactive fractions of the Lilium philadelphicum flower is limited and needs to be explored. The present study reports the antimicrobial potential of the bioactive fraction extracted from the flower of L. philadelphicum (red lily) and partial characterization of the bioactive compound(s). The antimicrobial activity was tested against nine different Gram-positive and Gram-negative bacterial strains. The minimum inhibitory concentration (MIC) values of methanolic extract of the L. philadelphicum flower against Acinetobacter bouvetii, Achromobacter xylosoxidans, Bacillus subtilis MTCC 121, Candida albicans MTCC 183, Klebsiella pneumoniae MTCC 3384, and Salmonella typhi MTCC 537 were 25, 50, 12.5, 50, 100, and 50 μg/mL, respectively. The phytochemical analysis of the extract revealed the presence of phenols, flavonoids, tannins, terpenoids, glycosides, coumarins, and quinones. The cytotoxicity of the partially purified compound against the HepG2 cell line using MTT assay demonstrated up to 90% cell viability with a bioactive compound concentration of 50 μg/mL. However, the increase in the bioactive compound’s concentration up to 1000 μg/mL resulted in nearly 80% cell viability. This minor decline in cell viability suggests the importance and suitability of the bioactive compound for therapeutic applications. Spectroscopic studies of the bioactive compound by UV-visible spectroscopy, FT-infrared spectroscopy, gas chromatography-mass spectrometry (GC-MS), as well as phytochemical analysis, suggested the presence of a terpenoid moiety, which may be responsible for the antimicrobial property of the L. philadelphicum flower.

scholarly journals Aktivitas antibakteri Pleurotus ostreatus varietas Grey Oyster pada Staphylococcus aureus dan Pseudomonas aeruginosa

2018 ◽  
Vol 6 (3) ◽  
pp. 387
Author(s):  
Evi Hanizar ◽  
Dwi Nur Rikhma Sari
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Pleurotus Ostreatus ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Phytochemical Analysis ◽  
Antimicrobial Potential ◽  
Treatment Variation ◽  
Experimental Type ◽  
The Difference ◽  
Bacterial Growth Inhibition

  Oyster mushroom Pleurotus ostreatus varieties Gray oyster is often consumed by the community because it tastes delicious and contains nutrients for health but utilization for medical treatment has not been studied. The aim of this research was to analyze antimicrobial potential compounds in P. ostreatus and to investigate the inhibitory effect on the growth of Staphyllococcus aureus and Pseudomonas aeruginosa. This research was experimental type with treatment variation 0.1; 0.25; 0.5; 1; 1.5 and 2% of P. ostreatus extract, with 5 replicates. Antimicrobial potential compounds was analyzed by qualitative phytochemical test while antibacterial activity evaluated by calculating the inhibition zone diameter presented as the bright zone formed in the surroundings paper discs on bacterial cultures using solid medium. Phytochemical analysis obtained positive results for flavonoid, tannin and terpenoid compounds. The effect of mushroom extract on the bacterial growth inhibition were analyzed by One Way Anova test. The result showed that the inhibitory growth diameter for both bacteria was not significantly different. It meant that the difference in concentration variations of P. ostreatus extracts of 0.25 and 0.50 did not cause different inhibitory effects on S. aureus and P aeruginosa bacteria and 0.1% concentration was capable of causing inhibitory effect.  

Phytochemical analysis of Anchusa arvensis roots

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
SK Tsermentseli ◽  
AN Assimopoulou ◽  
N Gianovits-Argyriadou ◽  
FI Kanaze ◽  
VP Papageorgiou
Keyword(s):  
Phytochemical Analysis
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