Pengembangan Biosensor Berbasis Plastik Zona Mikro untuk Skrining Aktivitas Antidiabetes pada Ekstrak Tanaman Obat

Research on the diabetes mellitus (DM) treatment has recently been carried out, especially on discovering antidiabetic agents derived from plants. This study aims to determine the fabrication of microzone plastic-based biosensors, determine the optimal sensor conditions, analysis characteristics, and compare the biosensor with the UV-Vis spectrophotometric method. The results showed that the response time was 15 minutes, the linear range was 500-40000 µg / mL (R = 0.9989). The limit of detection (LOD) was 1109.6 µg / mL, and the limit of quantification (LOQ) was 3698.8 µg / mL. Biosensor filled up the precision parameters with an RSD value of less than 3.7% and an accuracy with recovery in the range of 95-105%. The biosensor is stable in storage at 25 ° C for 270 minutes and at chiller temperature for three days. The antidiabetic activity of the biosensor was compared with the UV-Vis spectrophotometer using the Independent Sample T-test and showed insignificant differences between the two methods.

Optimasi Hidroksipropil Metilselulosa dan Polivinil Pirolidon dalam Sediaan Mucoadhesive Buccal Film Diltiazem Hidroklorida

Diltiazem HCl is a class of benzodiazepine calcium channel blockers used to treat angina pectoris, arrhythmias, and hypertension. Diltiazem HCl improves first-pass metabolism, a short half time of 3-5 hours, and bioavailability of diltiazem for oral administration of about 40%. Mucoadhesive buccal film diltiazem HCl releases the drug to the buccal mucosa, so the first pass metabolism can be avoided because of its absorption through the venous system that flows from the cheek. This study aimed to study HPMC polymers and polyvinyl pyrrolidone (PVP) polymers on swelling index, residence time, mucoadhesive strength of mucoadhesive buccal film diltiazem HCl, FTIR, and drug release. The prepared film was evaluated for swelling index, mucoadhesive strength, and mucoadhesive residence time. The optimal amount for HPMC was 35 mg, and PVP was 15 mg. The combination of polymers with this amount can produce a swelling index was 3,00, mucoadhesive strength was 41,87 gF, and mucoadhesive residence time was 330,66. FTIR test indicated that there was no interaction between active function clusters of Diltiazem HCl and other excipients. The release of the optimum formula in the 360th minute was about 97.847%, following in the zero-order release model and Higuchi.

Higiene sanitasi makanan dan analisis nomor P-IRT pada kerupuk berwarna merah (Studi pasar Kepanjen Kabupaten Malang)

Food sanitation hygiene has been done to control food factor, people, place and tools in order to prevent cross contamination between food and eater. Household food industry must possess household food industry certificate or SPP-IRT to aid supervision by involved parties. The aim of this research was to discover food sanitation hygiene and analysis P-IRT number on red-coloring crackers at Kepanjen Market in Malang. Thisresearch is descriptive research with observation and interview methods to cracker industry workers and there were 12 kinds of red-coloring crackers distributed around Kepanjen Market. The research used univariant analysis to describe variable of those which were observed. This research it was found that there were three stages in cracker making process, Food sanitation hygiene, and P-IRT number on red-coloring crackers. From this research there were 3 of respondents around < 40 years old, all respondents in secondary education, and 2respondents had < 3 years of working period. The application of food hygiene and sanitation of cracker industry in Malang Regency was categorized as relatively good. There were 7 red-coloring crackers traded at Kepanjen Market were qualified as what had been standardized by BPOM number 22 year 2020. Meanwhile, there were 4 products were not updated and 1 product was not qualified.

Faktor yang Mempengaruhi Persepsi Kemudahan dan Kemanfaatan Penggunaan Sistem Informasi Manajemen RSU Kaliwates Jember Tahun 2019

Kaliwates General Hospital is a privately owned public service body with full accreditation status for five services and was established by the Ministry of Health of the Republic of Indonesia on December 31, 2010, No.Y.M.01.10/III/7957/10. At the beginning of its development, Kaliwates General Hospital managed data and information using still manual systems. In 2012 Kaliwates General Hospital began using computerized management information systems and changed vendors in 2015. This research is an analytical study with an observational approach. In this study, researchers tested hypotheses on the influence of institutional factors, social factors, and individual factors independently and together influenced the perceived ease and prestige of the usefulness of information systems. Institutional factors, social factors, individual factors, and ease of use significantly affect perceived usefulness in the Kaliwates general hospital management information system.

Hubungan Tingkat Stres dengan Kualitas Tidur pada Ibu Preeklamsi di Wilayah Kerja Puskesmas Tempurejo-Jember

Preeclampsia is some of the symptoms that occur in pregnancy, childbirth, and puerperal women, which are characterized by hypertension, edema, and increased proteinuria. Preeclampsia usually occurs when pregnancy enters the age of 20 weeks to 48 hours after the birth process. In that condition, there are undesirable complications during pregnancy that can make mothers more vulnerable to psychological disorders, one of which is stress. Stress experienced during pregnancy can affect the health of the baby and can also improve poor sleep quality. This study aims to analyze the relationship of stress levels with the quality of preeclampsia sleep in the Tempurejo Community Health Center in Jember. This research was conducted on 31 preeclampsia mothers using a correlational research design with a cross-sectional approach and total sampling techniques. Data collection using questionnaires stress and sleep quality. The results showed 19 (61.3)% of respondents experienced mild stress, and 26 (83.9)% experienced poor sleep quality. The results of data analysis using the spearmen statistical test obtained p-value 0.001 and r = 0.894, which means there is a relationship between stress levels and sleep quality or ha is accepted. R-value = 0.396 means that the correlation between stress levels and sleep quality in preeclampsia has a positive correlation with weak correlation strength. Nursing implications for further research can intervene in interventions that can reduce stress levels with the quality of sleep in preeclampsia mothers to reduce the factors of preeclampsia.

Pengembangan Sensor Kimia Berbasis Strip Tes untuk Deteksi Logam Berat Merkuri pada Sampel Produk Jamu

The use of plants as a traditional medicine in the modern era is still in high demand. One of the herbs commonly used by Indonesian is analgesic herbal medicine. The safety aspect of herbal medicine should be considered, such as heavy metal contamination. Mercury is the typical heavy metal found in polluting herbal plants. The maximum mercury level allowed in a medicinal preparation has been regulated by BPOM RI, as much as ≤ 0.5 ppm. A high level of mercury contamination causing toxic effects on the body due to protein precipitation, which inhibits enzyme activity and acts as a corrosive material. A technology that allows the detection of mercury is a chemical sensor. The strip test is optimal if the reagent 1-(2-thiazolyl azo)-2-naphthol (TAN) concentration at 1000 ppm and the sample has been confirmed at pH 7. The results of the strip test characterization showed that the response time was in the range of minutes 17 to 24, the use time of the test strips for 28 days if stored in room temperature and 29 days if stored at in refrigerator temperature, linearity with r = 0.998, Vx0 = 3.96%, p-value = 0.00000172, LOD = 0.102 ppm and LOQ = 0.306 ppm, interference value <5%, precision with RSD 6.54%, and accuracy with the value of % recovery 98.007%. T-test analysis showed p= 0.872, meaning no significant difference between the application of TAN-based test strips with the ICP-AES method.

Peran Petugas Lingkungan Pondok Sosial (Liposos) dalam Pengelolaan Diapres pada Klien di UPTD Liposos Kabupaten Jember

Diapers merupakan alat yang berupa popok sekali pakai berdaya serap tinggi yang terbuat dari plastik dan campuran bahan kimia untuk menampung sisa-sisa metabolisme seperti air seni dan feses. Diapres masuk dalam limbah bahan berbahaya dan beracun yang disebut limbah B3. Limbah B3 adalah sisa suatu usaha atau kegiatan yang mengandung B3. Penggunaan diapers dewasa pada orang tua dan orang dengan gangguan jiwa yang tidak memiliki kesadaran dan mengalami pelemahan fisik banyak digunakan sebagai alternatif kontrol kemih, sebanyak 42,3% orang yang dirawat dirumah sakit yang mengalami penurunan mobilitas, kemampuan fisik dan kesadaan menggunakan diapers dewasa sebagai alternatif kontrol kemih dalam proses perawatan. Penelitian ini merupakan penelitian kuantitatif yang bertujuan untuk menganalisis peran petugas lingkungan pondok sosial (LIPOSOS) dalam pengelolaan diapers pada klien ODGJ di UPTD. Hasil penelitian menunjukkan bahwa dari 5 responden penelitian dalam pengelolaan diapers di UPTD. LIPOSOS yang digunakan yaitu dengan cara penimbunan, penguburan dan pembakaran. Sehingga dari penelitian yang dilakukan dapat disimpulkan bahwa belum ada pengelolalan secara baik dan benar mengenai pengolahan diapers di UPTD. LIPOSOS. Pemilihan diapers kurang memadai terkait dengan ukuran dan merk yang digunakan.

Daya Hambat Ekstrak Kulit Semangka (Citrullus lanatus) Terhadap Pertumbuhan Candida albicans

Candida albicans is normal flora found in the oral mucosa in healthy individuals but has opportunistic pathogenic properties. The change in the oral cavity's environmental conditions can cause C. albicans to turn into a pathogen and cause oral candidiasis. Antifungal drugs that are common used for the treatment of oral candidiasis are topical nystatin preparations. Nystatin is an antifungal drug from the polyene group produced by Streptomyces noursei, which is thought to be very effective in treating diseases caused by C. albicans with a success rate 79.6 - 87.5%. The previous study showed that watermelon peel extract contains active compounds of alkaloids, flavonoids, tannins, and saponins. The active compound is known to have antifungal activity. The study aimed to determine the ability of watermelon peel extract to inhibit the growth of C. albicans. The type of research conducted is experimental laboratories using the post-test only control group design. The observations in this study about the inhibitory power of watermelon rind extract (Citrullus lanatus) on the growth of C. albicans showed that there were inhibitory zones of watermelon skin extract on the growth of C. albicans.

Uji Aktivitas Antibakteri Ekstrak Etanol Daun Waru Gunung (Hibiscus macrophyllus) dan Fraksinya terhadap Staphylococcus aureus

Infection is one of the main causes of world health problems, especially in developing countries like Indonesia, one of which is an acute respiratory infection caused by bacteria such as Staphylococcus aureus. Therapeutics are used for the treatment of infections today with antibiotics. However, there are many cases of bacterial resistance to antibiotics that need to develop alternatives derived from plants. One of the plants that have antibacterial activity is the leaves of Waru Gunung (Hibiscus macrophyllus). The purpose of this study was to determine whether the ethanolic extract of H. macrophyllus leaves and their fractions have antibacterial activity against S. aureus. The method used in this antibacterial activity test is the disk diffusion method with a test concentration of 5%, 10%, 15%, 20%, and 25%. The results of this study indicate that all test samples have antibacterial activity except in the water fraction. The highest antibacterial activity was obtained in the ethyl acetate fraction which attracted semipolar compounds in the ethanolic extract of H. macrophyllus leaves. The antibacterial activity of the test samples in sequence from high to low includes ethyl acetate fraction, ethanolic extract, n-hexane fraction, and water fraction.

Aktivitas Antibakteri Kombinasi Vitamin C dan Amoksisilin sebagai Bahan Alternatif Intrakanal Medikamen terhadap Enterococcus faecalis secara In Vitro

Bacteria that are often found in cases of pulp necrosis and root canal treatment are Enterococcus faecalis. Elimination of microorganisms in the root canal can use intracanal medicament. The intracanal medicament can be used from antibiotics such as amoxicillin. The antibacterial power of amoxicillin can be increased by combination with vitamin C to prevent resistance. This research aimed to determine the zone of inhibition of vitamin C and amoxicillin against Enterococcus faecalis. The method used in this study is well diffusion method. Inhibition is shown through the clear zone around the wellbore at 24 hours, 48 ​​hours, 72 hours after incubation. The number of research samples is 16 consisted of 4 groups consisting of amoxicillin, vitamin C, a combination of vitamin C and amoxicillin, and 3 MIX MP. The data were tested by non-parametric statistical analysis Kruskal Wallis and Mann Whitney. The result showed that vitamin C and amoxicillin's antibacterial activity as alternative material intracanal medicament is equivalent to antibacterial 3 MIX MP.