Heterogeneity of Bacterial Community Compositions in Surface Sediments of Three Lake Zones in Lake Taihu

ABSTRACT Bacterial communities play crucial roles in the biogeochemical cycle of the surface sediments of freshwater lakes, but previous studies on bacterial community changes in this habitat have mostly been based on the total bacterial community (DNA level), while an exploration of the active microbiota at the RNA level has been lacking. Herein, we analysed the bacterial communities in the surface sediments of Lake Taihu at the DNA and RNA levels. Using MiSeq sequencing and real-time quantification, we found that the sequencing and quantitative results obtained at the RNA level compared with the DNA level were more accurate in responding to the spatiotemporal dynamic changes of the bacterial community. Although both sequencing methods indicated that Proteobacteria, Chloroflexi, Acidobacteria, Nitrospirae, Bacteroidetes and Actinobacteria were the dominant phyla, the co-occurrence network at the RNA level could better reflect the close relationship between microorganisms in the surface sediment. Additionally, further analysis showed that Prochlorococcus and Microcystis were the most relevant and dominant genera of Cyanobacteria in the total and active bacterial communities, respectively; our results also demonstrated that the analysis of Cyanobacteria-related groups at the RNA level was more ‘informative’.

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Characterization of the bacterial community composition in a hypoxic zone induced by Microcystis blooms in Lake Taihu, China

FEMS Microbiology Ecology ◽

10.1111/j.1574-6941.2011.01262.x ◽

2012 ◽

Vol 79 (3) ◽

pp. 773-784 ◽

Cited By ~ 40

Author(s):

Huabing Li ◽

Peng Xing ◽

Qinglong L. Wu

Keyword(s):

Bacterial Community ◽

Community Composition ◽

Lake Taihu ◽

Bacterial Community Composition ◽

Hypoxic Zone ◽

Microcystis Blooms

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Decomposition ofMicrocystisblooms: Implications for the structure of the sediment bacterial community, as assessed by a mesocosm experiment in Lake Taihu, China

Journal of Basic Microbiology ◽

10.1002/jobm.201100532 ◽

2012 ◽

Vol 53 (6) ◽

pp. 549-554 ◽

Cited By ~ 20

Author(s):

Keqiang Shao ◽

Guang Gao ◽

Kexu Chi ◽

Boqiang Qin ◽

Xiangming Tang ◽

...

Keyword(s):

Bacterial Community ◽

Lake Taihu ◽

Mesocosm Experiment ◽

Sediment Bacterial Community

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Removal of Microcystin-LR by a Novel Native Effective Bacterial Community Designated as YFMCD4 Isolated from Lake Taihu

Toxins ◽

10.3390/toxins10090363 ◽

2018 ◽

Vol 10 (9) ◽

pp. 363 ◽

Cited By ~ 23

Author(s):

Fei Yang ◽

Jian Guo ◽

Feiyu Huang ◽

Isaac Massey ◽

Ruixue Huang ◽

...

Keyword(s):

Bacterial Community ◽

High Performance ◽

Lake Taihu ◽

Degradation Process ◽

Alcaligenes Faecalis ◽

Natural Ecosystem ◽

The Novel ◽

Bacterial Strains ◽

Different Temperatures ◽

Cost Efficient

Microcystin-LR (MC-LR) is the most toxic and frequently detected monocyclic heptapeptide hepatotoxin produced by cyanobacteria, which poses a great threat to the natural ecosystem and public health. It is very important to seek environment-friendly and cost-efficient methods to remove MC-LR in water. In this study, the MC-degrading capacities of a novel indigenous bacterial community designated as YFMCD4 and the influence of environmental factors including various temperatures, MC concentrations and pH on the MC-degrading activities were investigated utilizing high-performance liquid chromatography (HPLC). In addition, the MC-degrading mechanism of YFMCD4 was also studied using HPLC coupled with a mass spectrometry equipped with electrospray ionization interface (HPLC-ESI-MS). The data showed MC-LR was completely removed at the maximum rate of 0.5 µg/(mL·h) under the optimal condition by YFMCD4. Two pure bacterial strains Alcaligenes faecalis and Stenotrophomonas acidaminiohila were isolated from YFMCD4 degraded MC-LR at a slower rate. The MC-degrading rates of YFMCD4 were significantly affected by different temperatures, pH and MC-LR concentrations. Two intermediates of a tetrapeptide and Adda appeared in the degradation process. These results illustrate that the novel YFMCD4 is one of the highest effective MC-degrading bacterial community, which can completely remove MC-LR and possesses a significant potential to treat water bodies contaminated by MC-LR.

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Comparing sediment bacterial communities in the macrophyte-dominated and algae-dominated areas of eutrophic Lake Taihu, China

Canadian Journal of Microbiology ◽

10.1139/w11-003 ◽

2011 ◽

Vol 57 (4) ◽

pp. 263-272 ◽

Cited By ~ 37

Author(s):

Keqiang Shao ◽

Guang Gao ◽

Boqiang Qin ◽

Xiangming Tang ◽

Yongping Wang ◽

...

Keyword(s):

Environmental Factors ◽

Bacterial Community ◽

Dna Sequences ◽

Lake Taihu ◽

Bacterial Community Composition ◽

Eutrophic Lake ◽

Banding Patterns ◽

Factors Affecting ◽

Specific Variation ◽

Area Variation

Bacterial community structure and the effects of several environmental factors on bacterial community distribution were investigated in the sediment of the macrophyte-dominated and algae-dominated areas in a large, shallow, eutrophic freshwater lake (Lake Taihu, China). Surface sediment samples were collected at 6 sampling sites (3 sites from each of the 2 areas) on 15 February and 15 August 2009. Based on cluster analysis of the DGGE banding patterns, there were significant seasonal variations in the structure of the sediment bacterial community in the macrophyte- and algae-dominated areas, and site-specific variation within an area and between 2 areas. However, there were no significant between-area variations due to the large within-area variation. Analysis of DNA sequences showed that there were differences in the species composition of the sediment bacteria between the macrophyte- and algae-dominated area clone libraries. In the macrophyte-dominated area library, the bacterial community was dominated by Deltaproteobacteria, Verrucomicrobia, Acidobacteria, Bacteroidetes, Gammaproteobacteria, and Betaproteobacteria. OP10 was found in the library of this area but not in the algae-dominated area library. The algae-dominated area library was dominated by Betaproteobacteria, Deltaproteobacteria, Gammaproteobacteria, and Acidobacteria. Cyanobacteria, Alphaproteobacteria, and Planctomycetes were found in this area library but not in the macrophyte-dominated area library. Canonical correspondence analysis demonstrated that total phosphorus and water temperature were the dominant environmental factors affecting bacterial community composition in the sediment.

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Organic phosphorus species in surface sediments of a large, shallow, eutrophic lake, Lake Taihu, China

Environmental Pollution ◽

10.1016/j.envpol.2009.03.018 ◽

2009 ◽

Vol 157 (8-9) ◽

pp. 2507-2513 ◽

Cited By ~ 111

Author(s):

Xiuling Bai ◽

Shiming Ding ◽

Chengxin Fan ◽

Tao Liu ◽

Dan Shi ◽

...

Keyword(s):

Surface Sediments ◽

Organic Phosphorus ◽

Lake Taihu ◽

Eutrophic Lake ◽

Phosphorus Species ◽

Shallow Eutrophic Lake

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Distribution, sources and ecological risk assessment of heavy metals in surface sediments from Lake Taihu, China

Environmental Research Letters ◽

10.1088/1748-9326/6/4/044012 ◽

2011 ◽

Vol 6 (4) ◽

pp. 044012 ◽

Cited By ~ 61

Author(s):

Hongbin Yin ◽

Yongnian Gao ◽

Chengxin Fan

Keyword(s):

Heavy Metals ◽

Risk Assessment ◽

Ecological Risk ◽

Ecological Risk Assessment ◽

Surface Sediments ◽

Lake Taihu

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Effects of benthic macrofauna bioturbation on the bacterial community composition in lake sediments

Canadian Journal of Microbiology ◽

10.1139/cjm-2014-0132 ◽

2014 ◽

Vol 60 (8) ◽

pp. 517-524 ◽

Cited By ~ 17

Author(s):

Jin Zeng ◽

Da-Yong Zhao ◽

Peng Liu ◽

Zhong-Bo Yu ◽

Rui Huang ◽

...

Keyword(s):

Bacterial Community ◽

Community Composition ◽

Lake Taihu ◽

Bacterial Community Composition ◽

Chemical Properties ◽

Eutrophic Lake ◽

Benthic Macrofauna ◽

Chironomidae Larvae ◽

Culture Independent ◽

Tubificid Worms

Benthic macrofauna are considered to be an important part of the lacustrine ecosystem, and bioturbation may greatly affect the biogeochemical processes and microbial activities in sediments. In the present study, the bacterial community composition in sediments inhabited by 3 different types of benthic macrofauna (Corbicula fluminea, Chironomidae larvae, and tubificid worms) in the shallow and eutrophic Lake Taihu was studied to investigate the different effects of bioturbation on the composition of these communities. Microcosms were constructed, and culture-independent methods, including terminal restriction fragment length polymorphism (T-RFLP) and clone library analysis, were performed to evaluate the bacterial communities. Analysis of similarities (ANOSIM) and multidimensional scaling (MDS) analysis of T-RFLP patterns demonstrated that differences in the bacterial community composition between the control and the macrofauna-inhabited sediments were not as great as expected, although the chemical properties of the sediments changed remarkably. Nevertheless, the dominant bacterial group in each type of macrofauna-inhabited sediment was different. Acidobacteria, Betaproteobacteria, and Deltaproteobacteria were the dominant bacterial groups in sediments inhabited by C. fluminea, tubificid worms, and Chironomidae larvae, respectively. The data obtained in this study are helpful for understanding the effects of bioturbation in a shallow, eutrophic lake.

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