Abstract. As the satellite microwave remote sensed brightness temperature is sensitive to land surface soil moisture (SM) and SM is a basic output variable in model simulation, it is of great significance to use the brightness temperature data to improve SM numerical simulation. In this paper, the theory developed by Yan et al. (2004) about the relationship between satellite microwave remote sensing polarization index and SM was used to estimate the land surface SM from AMSR-E (Advanced Microwave Scanning Radiometer – Earth Observing System) brightness temperature data. With consideration of land surface soil texture, surface roughness, vegetation optical thickness, and the AMSR-E monthly SM products, the regional daily land surface SM was estimated over the eastern part of the Qinghai-Tibet Plateau. The results show that the estimated SM is lower than the ground measurements and the NCEP (American National Centers for Environmental Prediction) reanalysis data at the Maqu Station (33.85° N, 102.57° E) and the Tanglha Station (33.07° N, 91.94° E), but its regional distribution is reasonable and somewhat better than that from the daily AMSR-E SM product, and its temporal variation shows a quick response to the ground daily precipitations. Furthermore, in order to improve the simulating ability of the WRF (Weather Research and Forecasting) model to land surface SM, the estimated SM was assimilated into the Noah land surface model by the Newtonian relaxation (NR) method. The results indicate that, by fine tuning of the quality factor in NR method, the simulated SM values are improved most in desert area, followed by grassland, shrub and grass mixed zone. At temporal scale, Root Mean Square Error (RMSE) values between simulated and observed SM are decreased 0.03 and 0.07 m3/m3 by using the NR method in the Maqu Station and the Tanglha Station, respectively.