OsWNK9 mitigates salt and drought stress effects through induced antioxidant systems in Arabidopsis

Melatonin is a biological hormone that plays crucial roles in stress tolerance. In this study, we investigated the effect of exogenous melatonin on abiotic stress in the tea plant. Under cold, salt and drought stress, increasing malondialdehyde levels and decreasing maximum photochemical efficiency of PSII were observed in tea leaves. Meanwhile, the levels of reactive oxygen species (ROS) increased significantly under abiotic stress. Interestingly, pretreatment with melatonin on leaves alleviated ROS burst, decreased malondialdehyde levels and maintain high photosynthetic efficiency. Moreover, 100 μM melatonin-pretreated tea plants showed high levels of glutathione and ascorbic acid and increased the activities of superoxide dismutase, peroxidase, catalase and ascorbate peroxidase under abiotic stress. Notably, melatonin treatments can positively up-regulate the genes (CsSOD, CsPOD, CsCAT and CsAPX) expression of antioxidant enzyme biosynthesis. Taken together, our results confirmed that melatonin protects tea plants against abiotic stress-induced damages through detoxifying ROS and regulating antioxidant systems.

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Physiological and Proteomic Responses of Pitaya to PEG-Induced Drought Stress

Agriculture ◽

10.3390/agriculture11070632 ◽

2021 ◽

Vol 11 (7) ◽

pp. 632

Author(s):

Aihua Wang ◽

Chao Ma ◽

Hongye Ma ◽

Zhilang Qiu ◽

Xiaopeng Wen

Keyword(s):

Drought Stress ◽

Energy Metabolism ◽

Metabolic Pathways ◽

Treatment Time ◽

Soluble Sugar ◽

Antioxidant Systems ◽

Antioxidant Enzyme Activities ◽

Proteomic Data ◽

Proteome Profile ◽

Proteomic Responses

Pitaya (Hylocereus polyrhizus L.) is highly tolerant to drought stress. Elucidating the response mechanism of pitaya to drought will substantially contribute to improving crop drought tolerance. In the present study, the physiological and proteomic responses of the pitaya cultivar ‘Zihonglong’ were compared between control seedlings and seedlings exposed to drought stress (−4.9 MPa) induced by polyethylene glycol for 7 days. Drought stress obviously enhanced osmolyte accumulation, lipid peroxidation, and antioxidant enzyme activities. Proteomic data revealed drought stress activated several pathways in pitaya, including carbohydrate and energy metabolism at two drought stress treatment time-points (6 h and 3 days). Other metabolic pathways, including those related to aspartate, glutamate, glutathione, and secondary metabolites, were induced more at 3 days than at 6 h, whereas photosynthesis and arginine metabolism were induced exclusively at 6 h. Overall, protein expression changes were consistent with the physiological responses, although there were some differences in the timing. The increases in soluble sugar contents mainly resulted from the degradation and transformation of insoluble carbohydrates. Differentially accumulated proteins in amino acid metabolism may be important for the conversion and accumulation of amino acids. GSH and AsA metabolism and secondary metabolism may play important roles in pitaya as enzymatic and nonenzymatic antioxidant systems. The enhanced carbohydrate and energy metabolism may provide the energy necessary for initiating the above metabolic pathways. The current study provided the first proteome profile of this species exposed to drought stress, and may clarify the mechanisms underlying the considerable tolerance of pitaya to drought stress.

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Comparative analysis of drought stress effects on photosynthesis of Eurasian and North African genotypes of wild barley

Photosynthetica ◽

10.1007/s11099-014-0064-3 ◽

2014 ◽

Vol 52 (4) ◽

pp. 564-573 ◽

Cited By ~ 11

Author(s):

C. Jedmowski ◽

S. Bayramov ◽

W. Bruggemann

Keyword(s):

Comparative Analysis ◽

Drought Stress ◽

Wild Barley ◽

North African ◽

Stress Effects

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Role of Kinases for Regulating K + Homeostasis Under Salt and Drought Stress Conditions

Protein Kinases and Stress Signaling in Plants ◽

10.1002/9781119541578.ch21 ◽

2020 ◽

pp. 479-500

Author(s):

Jayant Kulkarni ◽

Ashish K. Srivastava ◽

Suprasanna Penna

Keyword(s):

Drought Stress ◽

Stress Conditions ◽

Salt And Drought Stress

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Proteome Analysis for Understanding Abiotic Stress (Salinity and Drought) Tolerance in Date Palm (Phoenix dactyliferaL.)

International Journal of Genomics ◽

10.1155/2015/407165 ◽

2015 ◽

Vol 2015 ◽

pp. 1-11 ◽

Cited By ~ 14

Author(s):

Haddad A. El Rabey ◽

Abdulrahman L. Al-Malki ◽

Khalid O. Abulnaja ◽

Wolfgang Rohde

Keyword(s):

Drought Stress ◽

Chlorophyll A ◽

Date Palm ◽

Light Harvesting ◽

Binding Protein ◽

Proteome Analysis ◽

Rubisco Activase ◽

Stress Conditions ◽

Spectrometric Analysis ◽

Salt And Drought Stress

This study was carried out to study the proteome of date palm under salinity and drought stress conditions to possibly identify proteins involved in stress tolerance. For this purpose, three-month-old seedlings of date palm cultivar “Sagie” were subjected to drought (27.5 g/L polyethylene glycol 6000) and salinity stress conditions (16 g/L NaCl) for one month. DIGE analysis of protein extracts identified 47 differentially expressed proteins in leaves of salt- and drought-treated palm seedlings. Mass spectrometric analysis identified 12 proteins; three out of them were significantly changed under both salt and drought stress, while the other nine were significantly changed only in salt-stressed plants. The levels of ATP synthase alpha and beta subunits, an unknown protein and some of RubisCO fragments were significantly changed under both salt and drought stress conditions. Changes in abundance of superoxide dismutase, chlorophyll A-B binding protein, light-harvesting complex1 protein Lhca1, RubisCO activase, phosphoglycerate kinase, chloroplast light-harvesting chlorophyll a/b-binding protein, phosphoribulokinase, transketolase, RubisCO, and some of RubisCO fragments were significant only for salt stress.

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