scholarly journals Proteome Analysis for Understanding Abiotic Stress (Salinity and Drought) Tolerance in Date Palm (Phoenix dactyliferaL.)

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Haddad A. El Rabey ◽  
Abdulrahman L. Al-Malki ◽  
Khalid O. Abulnaja ◽  
Wolfgang Rohde
Keyword(s):  
Drought Stress ◽  
Chlorophyll A ◽  
Date Palm ◽  
Light Harvesting ◽  
Binding Protein ◽  
Proteome Analysis ◽  
Rubisco Activase ◽  
Stress Conditions ◽  
Spectrometric Analysis ◽  
Salt And Drought Stress

This study was carried out to study the proteome of date palm under salinity and drought stress conditions to possibly identify proteins involved in stress tolerance. For this purpose, three-month-old seedlings of date palm cultivar “Sagie” were subjected to drought (27.5 g/L polyethylene glycol 6000) and salinity stress conditions (16 g/L NaCl) for one month. DIGE analysis of protein extracts identified 47 differentially expressed proteins in leaves of salt- and drought-treated palm seedlings. Mass spectrometric analysis identified 12 proteins; three out of them were significantly changed under both salt and drought stress, while the other nine were significantly changed only in salt-stressed plants. The levels of ATP synthase alpha and beta subunits, an unknown protein and some of RubisCO fragments were significantly changed under both salt and drought stress conditions. Changes in abundance of superoxide dismutase, chlorophyll A-B binding protein, light-harvesting complex1 protein Lhca1, RubisCO activase, phosphoglycerate kinase, chloroplast light-harvesting chlorophyll a/b-binding protein, phosphoribulokinase, transketolase, RubisCO, and some of RubisCO fragments were significant only for salt stress.

scholarly journals Proteome Analysis of Date Palm (Phoenix dactylifera L.) under Severe Drought and Salt Stress

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Haddad A. El Rabey ◽  
Abdulrahman L. Al-Malki ◽  
Khalid O. Abulnaja
Keyword(s):  
Salt Stress ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Date Palm ◽  
Proteome Analysis ◽  
Rubisco Activase ◽  
2D Electrophoresis ◽  
Severe Drought ◽  
Protein Abundance ◽  
Low Protein

Date palm cultivars differently tolerate salinity and drought stress. This study was carried out to study the response of date palm to severe salinity and drought based on leaf proteome analysis. Eighteen-month-old date palm plants were subjected to severe salt (48 g/L NaCl) and drought (82.5 g/L PEG or no irrigation) conditions for one month. Using a protein 2D electrophoresis method, 55 protein spots were analyzed using mass spectrometry. ATP synthase CF1 alpha chains were significantly upregulated under all three stress conditions. Changes in the abundance of RubisCO activase and one of the RubisCO fragments were significant in the same spots only for salt stress and drought stress with no irrigation, and oxygen-evolving enhancer protein 2 was changed in different spots. Transketolase was significantly changed only in drought stress with PEG. The expression of salt and drought stress genes of the chosen protein spots was either overexpressed or downexpressed as revealed by the high or low protein abundance, respectively. In addition, all drought tolerance genes due to no irrigation were downregulated. In conclusion, the proteome analysis of date palm under salinity and drought conditions indicated that both salinity and drought tolerance genes were differentially expressed resulting in high or low protein abundance of the chosen protein spots as a result of exposure to drought and salinity stress condition.

Role of Kinases for Regulating K + Homeostasis Under Salt and Drought Stress Conditions

2020 ◽  
pp. 479-500
Author(s):  
Jayant Kulkarni ◽  
Ashish K. Srivastava ◽  
Suprasanna Penna
Keyword(s):  
Drought Stress ◽  
Stress Conditions ◽  
Salt And Drought Stress

The N-terminal domain of the light-harvesting chlorophyll a/b -binding protein complex (LHCII) is essential for its acclimative proteolysis

FEBS Letters ◽  
2000 ◽  
Vol 466 (2-3) ◽  
pp. 385-388 ◽  
Author(s):  
Dan-Hui Yang ◽  
Harald Paulsen ◽  
Bertil Andersson
Keyword(s):  
Chlorophyll A ◽  
Protein Complex ◽  
Light Harvesting ◽  
Binding Protein ◽  
Terminal Domain

STAY-GREEN and light-harvesting complex II chlorophyll a/b binding protein are involved in albinism of a novel albino tea germplasm ‘Huabai 1’

2022 ◽  
Vol 293 ◽  
pp. 110653
Author(s):  
Xuefei Chen ◽  
Jianjie Li ◽  
Ying Yu ◽  
Xiaobing Kou ◽  
Rajiv Periakaruppan ◽  
...  
Keyword(s):  
Chlorophyll A ◽  
Light Harvesting ◽  
Binding Protein ◽  
Stay Green ◽  
Light Harvesting Complex ◽  
Complex Ii ◽  
Light Harvesting Complex Ii

scholarly journals Light-harvesting chlorophyll a/b-binding protein-coding genes in jatropha and the comparison with castor, cassava and arabidopsis

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8465 ◽  
Author(s):  
Yongguo Zhao ◽  
Hua Kong ◽  
Yunling Guo ◽  
Zhi Zou
Keyword(s):  
Chlorophyll A ◽  
Light Harvesting ◽  
Ricinus Communis ◽  
Binding Protein ◽  
Expression Profiles ◽  
Transcriptome Profiling ◽  
Protein Coding ◽  
Whole Genome Duplications ◽  
Genome Duplications ◽  
Species Specific

The Lhc (light-harvesting chlorophyll a/b-binding protein) superfamily represents a class of antennae proteins that play indispensable roles in capture of solar energy as well as photoprotection under stress conditions. Despite their importance, little information has been available beyond model plants. In this study, we presents a first genome-wide analysis of Lhc superfamily genes in jatropha (Jatropha curcas L., Euphorbiaceae), an oil-bearing plant for biodiesel purpose. A total of 27 members were identified from the jatropha genome, which were shown to distribute over nine out of the 11 chromosomes. The superfamily number is comparable to 28 present in castor (Ricinus communis, Euphorbiaceae), but relatively less than 35 in cassava (Manihot esculenta, Euphorbiaceae) and 34 in arabidopsis (Arabidopsis thaliana) that experienced one or two recent whole-genome duplications (WGDs), respectively. In contrast to a high number of paralogs present in cassava and arabidopsis, few duplicates were found in jatropha as observed in castor, corresponding to no recent WGD occurred in these two species. Nevertheless, 26 orthologous groups representing four defined families were found in jatropha, and nearly one-to-one orthologous relationship was observed between jatropha and castor. By contrast, a novel group named SEP6 was shown to have been lost in arabidopsis. Global transcriptome profiling revealed a predominant expression pattern of most JcLhc superfamily genes in green tissues, reflecting their key roles in photosynthesis. Moreover, their expression profiles upon hormones, drought, and salt stresses were also investigated. These findings not only improve our knowledge on species-specific evolution of the Lhc supergene family, but also provide valuable information for further studies in jatropha.

Cell membrane stability and chlorophyll content variation in wheat (Triticum aestivum) genotypes under conditions of heat and drought

2016 ◽  
Vol 67 (7) ◽  
pp. 712 ◽  
Author(s):  
Shoaib Ur Rehman ◽  
Muhammad Bilal ◽  
Rashid Mehmood Rana ◽  
Muhammad Naveed Tahir ◽  
Muhammad Kausar Nawaz Shah ◽  
...  
Keyword(s):  
Heat Stress ◽  
Drought Stress ◽  
Cell Membrane ◽  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Stress Conditions ◽  
Seedling Stage ◽  
Total Chlorophyll ◽  
Cell Membrane Stability ◽  
Total Chlorophyll Content

Heat and drought are among the major obstacles confronting crop production under climate change. The present study was conducted to evaluate 50 diverse wheat genotypes for cell membrane stability (CMS) and chlorophyll content at seedling and anthesis stages under heat and drought stress conditions, to understand the effect of the two abiotic factors and to find promising genotypes for future breeding. Experiments were conducted in the glasshouse (seedling stage) and the field (anthesis stage). Analysis of variance showed significant variation (P ≤ 0.05) for all of the traits at seedling and anthesis stages. High levels of broad-sense heritability and genetic advance at 5% selection intensity indicated the presence of a high genetic component of variation and potential for genetic improvement through selection among the existing genetic variation. CMS showed a significant positive correlation with 1000-grain weight (TGW) under heat and drought conditions at both seedling and anthesis stages. Chlorophyll a/b ratio at seedling stage exhibited a significant negative correlation (r = –0.39, P < 0.05) with TGW under heat stress. Total chlorophyll content was significantly (r = 0.42, P < 0.05) correlated with TGW under heat stress at anthesis. Genotypes ETAD248 and ETAD7 showed the highest CMS and TGW values, whereas their chlorophyll a/b values were lowest, at both seedling and anthesis stages under heat and drought stress conditions. Higher CMS and total chlorophyll content, and lower chlorophyll a/b, were found to be useful indicators to identify genotypes with high TGW under heat and drought stress conditions. This study indicated the possibility of using seedling resistance as an indicator for later stage response in breeding for heat and drought resistance. The resistant genotypes identified can be used as potential germplasm in breeding programs.

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