A Comparison of Genomic DNA Extraction Protocols in Artemisia annua L. for Large Scale Genetic Analyses Studies

2021 ◽  
Author(s):  
Negar Valizadeh ◽  
Hossein Abbasi Holasou ◽  
Seyyed Abolghasem Mohammadi ◽  
Khalid Mahmood Khawar
Keyword(s):  
Dna Extraction ◽  
Genomic Dna ◽  
Large Scale ◽  
Artemisia Annua ◽  
Genetic Analyses ◽  
Genomic Dna Extraction ◽  
Artemisia Annua L

scholarly journals A simple and efficient genomic DNA extraction protocol for large scale genetic analyses of plant biological systems

Plant Gene ◽  
2015 ◽  
Vol 1 ◽  
pp. 43-45 ◽  
Author(s):  
Kamirou Chabi Sika ◽  
Timnit Kefela ◽  
Hubert Adoukonou-Sagbadja ◽  
Leonard Ahoton ◽  
Aliou Saidou ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Genomic Dna ◽  
Large Scale ◽  
Biological Systems ◽  
Genetic Analyses ◽  
Genomic Dna Extraction ◽  
Extraction Protocol

scholarly journals Comparison of Eleven Methods for Genomic DNA Extraction Suitable for Large-Scale Whole-Genome Genotyping and Long-Term DNA Banking Using Blood Samples

PLoS ONE ◽  
2015 ◽  
Vol 10 (1) ◽  
pp. e0115960 ◽  
Author(s):  
Androniki Psifidi ◽  
Chrysostomos I. Dovas ◽  
Georgios Bramis ◽  
Thomai Lazou ◽  
Claire L. Russel ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Genomic Dna ◽  
Large Scale ◽  
Whole Genome ◽  
Blood Samples ◽  
Genomic Dna Extraction ◽  
Dna Banking

Optimization of genomic DNA extraction from formalin-fixed fish specimens

2013 ◽  
Vol 19 (6) ◽  
pp. 1068-1073
Author(s):  
Xiaolan KONG ◽  
Zuozhi CHEN ◽  
Lin LIN ◽  
Chunhou LI ◽  
Peiwen LIANG
Keyword(s):  
Dna Extraction ◽  
Genomic Dna ◽  
Genomic Dna Extraction ◽  
Formalin Fixed

Optimization of genomic DNA extraction from grapevine cultivars

2012 ◽  
Vol 29 ◽  
pp. S220
Author(s):  
Karlygash Aubakirova ◽  
Madina Omasheva ◽  
Natalya Ryabushkina ◽  
Laura Yerbolova ◽  
Tolepbergen Tazhibaev ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Genomic Dna ◽  
Genomic Dna Extraction

scholarly journals Optimization of BY2 cell suspension as a stable transformable system

2014 ◽  
Vol 42 (2) ◽  
pp. 472-477 ◽  
Author(s):  
Zhou SHUMIN ◽  
Chu YANXIA ◽  
Zheng BANG ◽  
Zhang WEI
Keyword(s):  
Cell Suspension ◽  
Dna Extraction ◽  
Genomic Dna ◽  
35S Promoter ◽  
Gfp Expression ◽  
Genomic Dna Extraction ◽  
Agrobacterium Mediated Transformation ◽  
Culture Cells ◽  
Low Efficiency

Tobacco (Nicotiana tabacum) cv. ‘Bright Yellow 2’ (BY2) cell suspension is a useful system to study the structure and function of plant cell. However, low efficiency of Agrobacterium-mediated transformation, and transgene silencing during subculture limit its application. Here we present optimization of the traditional protocols of Agrobacterium-mediated transformation and genomic DNA extraction. The transforming efficiency and recovery ratio of genomic DNA extraction were substantially increased by these improvements. Southern assay demonstrated that copy number of transgene could be determined unambiguously. Meanwhile by monitoring the GFP fluorescence we found that the GFP expression can keep stable in suspension culture cells for at least 20 days in liquid medium. Finally, applicability of constitutive promoters of Arabidopsis thaliana UBIQUITIN10 (AtUBQ10) and ARABIDOPSISSKP1 HOMOLOGUE1 (AtASK1) also can drive stable GFP expression in vivo of BY2 cells like CaMV 35S promoter in this plant system./span>

Genomic DNA extraction from herbarium samples of Cunila D. Royen ex L. (Lamiaceae) and Polygala L. (Polygalaceae)

2010 ◽  
Vol 3 (1) ◽  
pp. 37-39 ◽  
Author(s):  
Gustavo Agostini ◽  
Raquel Lüdtke ◽  
Sergio Echeverrigaray ◽  
Tatiana Teixeira de Souz-Chies
Keyword(s):  
Dna Extraction ◽  
Genomic Dna ◽  
Genomic Dna Extraction
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