Exogenous application of coat protein-specific dsRNA inhibits cognate cucumber mosaic virus (CMV) of ghost pepper

2022 ◽  
Author(s):  
Gowtham Kumar Routhu ◽  
Munmi Borah ◽  
Sundaresha Siddappa ◽  
P. D. Nath
Keyword(s):  
Coat Protein ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Exogenous Application

scholarly journals First Report of Cucumber mosaic virus Subgroup II Infecting Lycopersicon esculentum in India

Plant Disease ◽  
2006 ◽  
Vol 90 (11) ◽  
pp. 1457-1457 ◽  
Author(s):  
N. Sudhakar ◽  
D. Nagendra-Prasad ◽  
N. Mohan ◽  
K. Murugesan
Keyword(s):  
Coat Protein ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Tamil Nadu ◽  
Indian Subcontinent ◽  
Enzyme Linked Immunosorbent Assay ◽  
Infected Leaf ◽  
Polymorphism Analysis ◽  
First Report ◽  
Subgroup Ii

During a survey in January 2006 near Salem in Tamil Nadu (south India), Cucumber mosaic virus was observed infecting tomatoes with an incidence of more than 70%. Plants exhibiting severe mosaic, leaf puckering, and stunted growth were collected, and the virus was identified using diagnostic hosts, evaluation of physical properties of the virus, compound enzyme-linked immunosorbent assay (ELISA) (ELISA Lab, Washington State University, Prosser), reverse-transcription polymerase chain reaction (RT-PCR), and restriction fragment length polymorphism analysis (DSMZ, S. Winter, Germany). To determine the specific CMV subgroup, total RNA was extracted from 50 infected leaf samples using the RNeasy plant RNA isolation kit (Qiagen, Hilden, Germany) and tested for the presence of the complete CMV coat protein gene using specific primers as described by Rizos et al. (1). A fragment of the coat protein was amplified and subsequently digested with MspI to reveal a pattern of two fragments (336 and 538 bp), indicating CMV subgroup II. No evidence of mixed infection with CMV subgroup I was obtained when CMV isolates representing subgroups I (PV-0419) and II (PV-0420), available at the DSMZ Plant Virus Collection, were used as controls. Only CMV subgroup I has been found to predominantly infect tomato in the Indian subcontinent, although Verma et al. (2) identified CMV subgroup II infecting Pelargonium spp., an ornamental plant. To our knowledge, this is the first report of CMV subgroup II infecting tomato crops in India. References: (1) H. Rizos et al. J. Gen. Virol. 73:2099, 1992. (2) N. Verma et al. J. Biol. Sci. 31:47, 2006.

Expression of the gene encoding the coat protein of cucumber mosaic virus (CMV) strain WL appears to provide protection to tobacco plants against infection by several different CMV strains

Gene ◽  
1991 ◽  
Vol 107 (2) ◽  
pp. 181-188 ◽  
Author(s):  
Namba Shigetou ◽  
Ling Kaishu ◽  
Carol Gonsalves ◽  
Dennis Gonsalves ◽  
Jerry L. Slightom
Keyword(s):  
Coat Protein ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Gene Encoding ◽  
Tobacco Plants

Rapid transient protein production by the coat protein-deficient cucumber mosaic virus vector: non-packaged CMV system, NoPaCS

2018 ◽  
Vol 37 (11) ◽  
pp. 1513-1522 ◽  
Author(s):  
Noriho Fukuzawa ◽  
Chikara Masuta ◽  
Takeshi Matsumura
Keyword(s):  
Coat Protein ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Protein Production ◽  
Virus Vector

scholarly journals Cucumber mosaic virus coat protein induces the development of chlorotic symptoms through interacting with the chloroplast ferredoxin I protein

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Yanhong Qiu ◽  
Yongjiang Zhang ◽  
Chaonan Wang ◽  
Rong Lei ◽  
Yupin Wu ◽  
...  
Keyword(s):  
Coat Protein ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Virus Coat Protein ◽  
Ferredoxin I ◽  
Virus Coat

A reexamination of the molecular size of cucumber mosaic virus and its coat protein

Virology ◽  
1972 ◽  
Vol 49 (3) ◽  
pp. 647-653 ◽  
Author(s):  
M.H.V. van Regenmortel ◽  
D.A. Hendry ◽  
T. Baltz
Keyword(s):  
Coat Protein ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Molecular Size

Preparation of polyclonal antibody against recombinant coat protein of Cucumber mosaic virus isolate B13

2017 ◽  
Vol 6 (1) ◽  
pp. 25-34 ◽  
Author(s):  
Davoud Koolivand ◽  
◽  
Nemat Sokhandan Bashir ◽  
Afshin Rostami ◽  
◽  
...  
Keyword(s):  
Coat Protein ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Polyclonal Antibody ◽  
Virus Isolate ◽  
Cucumber Mosaic Virus Isolate ◽  
Recombinant Coat Protein

Monoclonal antibodies specific to Cucumber mosaic virus coat protein possess DNA-hydrolyzing activity

2009 ◽  
Vol 46 (7) ◽  
pp. 1527-1533 ◽  
Author(s):  
Haggag S. Zein ◽  
Jaime A. Teixeira da Silva ◽  
Kazutaka Miyatake
Keyword(s):  
Monoclonal Antibodies ◽  
Coat Protein ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Virus Coat Protein ◽  
Virus Coat
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