Characterization of Active Biomass and Species by Means of Respirometric Technique from Activated Sludge Models

2017 ◽  
Vol 11 (4) ◽  
pp. 489-500 ◽  
Author(s):  
Hana Benaliouche ◽  
Djamal Abdessemed ◽  
Geoffroy Lesage ◽  
Marc Heran
Keyword(s):  
Activated Sludge ◽  
Active Biomass ◽  
Activated Sludge Models

scholarly journals Determination of the heterotrophic and autotrophic active biomass during activated sludge respirometric batch assays using molecular techniques

2008 ◽  
Author(s):  
◽  
Arshad Ismail
Keyword(s):  
Activated Sludge ◽  
Direct Measurement ◽  
Molecular Techniques ◽  
Cod Removal ◽  
Active Biomass ◽  
Biomass Components ◽  
Nitrification And Denitrification ◽  
Activated Sludge Models

Activated sludge models now in use worldwide for the design and operation of treatment systems use hypothetical concentrations of active organisms. In order to validate and calibrate model outputs, concentrations and activities of organisms responsible for nitrification and denitrification need to be reflected by actual measurements. This research has been initiated by the observation of an increasing gap of suitable techniques that exist in the direct measurement and separation of active biomass components, responsible for COD removal and denitrification.

Enrichment and characterization of an anaerobic methyl ethyl ketoxime degrading culture from an anoxic/anaerobic/aerobic activated sludge sequencing batch reactor

1998 ◽  
Vol 37 (4-5) ◽  
pp. 95-98 ◽  
Author(s):  
Nancy G. Love ◽  
Mary E. Rust ◽  
Kathy C. Terlesky
Keyword(s):  
Energy Source ◽  
Activated Sludge ◽  
Electron Acceptor ◽  
Sequencing Batch Reactor ◽  
Enrichment Culture ◽  
Batch Reactor ◽  
Nitrification Inhibitor ◽  
Time Frame ◽  
Methyl Ethyl

An anaerobic enrichment culture was developed from an anoxic/anaerobic/aerobic activated sludge sequencing batch reactor using methyl ethyl ketoxime (MEKO), a potent nitrification inhibitor, as the sole carbon and energy source in the absence of molecular oxygen and nitrate. The enrichment culture was gradually fed decreasing amounts of biogenic organic compounds and increasing concentrations of MEKO over 23 days until the cultures metabolized the oxime as the sole carbon source; the cultures were maintained for an additional 41 days on MEKO alone. Turbidity stabilized at approximately 100 mg/l total suspended solids. Growth on selective media plates confirmed that the microorganisms were utilizing the MEKO as the sole carbon and energy source. The time frame required for growth indicated that the kinetics for MEKO degradation are slow. A batch test indicated that dissolved organic carbon decreased at a rate comparable to MEKO consumption, while sulfate was not consumed. The nature of the electron acceptor in anaerobic MEKO metabolism is unclear, but it is hypothesized that the MEKO is hydrolyzed intracellularly to form methyl ethyl ketone and hydroxylamine which serve as electron donor and electron acceptor, respectively.

Characterization of the fate of lipids in activated sludge

2008 ◽  
Vol 20 (5) ◽  
pp. 536-542 ◽  
Author(s):  
Kangala B. Chipasa ◽  
Krystyna Mdrzycka
Keyword(s):  
Activated Sludge

scholarly journals Characterization of Norovirus-Binding Proteins (NoVBPs) Recovered from Activated Sludge Microorganisms

2007 ◽  
Vol 30 (12) ◽  
pp. 731-736
Author(s):  
Keishi WADA ◽  
Daisuke SANO ◽  
Takahiro IMAI ◽  
Tatsuo OMURA
Keyword(s):  
Activated Sludge ◽  
Binding Proteins

Estimation of Kinetic Parameters of Heterotrophic Biomass under Aerobic Conditions and Characterization of Wastewater for Activated Sludge Modelling

1992 ◽  
Vol 25 (6) ◽  
pp. 125-139 ◽  
Author(s):  
J. Kappeler ◽  
W. Gujer
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Kinetic Parameters ◽  
Specific Growth ◽  
Wastewater Treatment Plants ◽  
Simple Method ◽  
Batch Tests ◽  
Different Types ◽  
Heterotrophic Biomass

To predict the behaviour of biological wastewater treatment plants, the Activated Sludge Model No. 1 is often used. For the application of this model kinetic parameters and wastewater composition must be known. A simple method to estimate kinetic parameters of heterotrophic biomass and COD wastewater fractions is presented. With three different types of batch-tests these parameters and fractions can be determined by measuring oxygen respiration. Our measurements showed that the maximum specific growth rate µmax of heterotrophic biomass depends on temperature, reactor configuration and SRT. In typical wastewater treatment plants of Switzerland the amount of readily biodegradable substrate was generally small (about 9 % of the COD in primary effluent). The same method can also be used to determine kinetic parameters of nitrifying biomass.

A simplified method to assess structurally identifiable parameters in Monod-based activated sludge models

2003 ◽  
Vol 37 (12) ◽  
pp. 2893-2904 ◽  
Author(s):  
Britta Petersen ◽  
Krist Gernaey ◽  
Martijn Devisscher ◽  
Denis Dochain ◽  
Peter A. Vanrolleghem
Keyword(s):  
Activated Sludge ◽  
Simplified Method ◽  
Activated Sludge Models

Characterization of wastewater and activated sludge from European municipal wastewater treatment plants using the NUR test

1998 ◽  
Vol 38 (1) ◽  
pp. 303-310 ◽  
Author(s):  
V. Naidoo ◽  
V. Urbain ◽  
C. A. Buckley
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
Municipal Wastewater Treatment ◽  
Batch Tests ◽  
Denitrification Kinetics ◽  
Municipal Wastewater Treatment Plants

Denitrification kinetics and wastewater characterization of eight different plants in Europe are discussed. Denitrification batch tests revealed three distinct rates except in the cases of Plaisir, Rostock and Orense where 4 rates were observed. The latter three plants revealed atypical rapid initial rates which were between 7 and 21 mgN/gVSS.h. All denitrification kinetics under non-limiting carbon conditions revealed fast first rates which ranged between 3.0 and 7.3 mgN/gVSS.h. Acetate was used to simulate denitrification kinetics with readily biodegradable COD present. Two subsequent rates were observed. Rates 2 and 3 ranged between 2 and 3 mgN/gVSS.h, and 1 and 2 mgN/gVSS.h, respectively. The RBCOD fraction varied between 10 and 19%, except for one of the plants where the value determined was 7%.

Physicochemical and Biological Treatability Studies on Surfactant Wastewaters

1992 ◽  
Vol 26 (1-2) ◽  
pp. 377-386 ◽  
Author(s):  
F. Çeçen
Keyword(s):  
Activated Sludge ◽  
Suspended Solids ◽  
Heavy Duty ◽  
Surface Active Agents ◽  
Surface Active ◽  
Liquid Type ◽  
Experimental Findings ◽  
Biological Treatability ◽  
Anionic Surface

Laboratory-scale physicochemical and biological treatability studies were performed on wastewaters discharged from an industry producing household and heavy-duty detergents of powder and liquid type. The characterization of effluents led to the conclusion that the industry was highly pollutant in terms of BOD5, COD, surfactant, phosphorus, oil-grease, suspended solids and pH. By the use of lime in the precipitation stage about 80 % of COD and more than 90 % of phosphorus and anionic surface active agents could be removed. Following the treatment with lime the biodegradation of these wastewaters was investigated in continuously fed activated sludge units. The biokinetic constants of the reaction were determined as k = 0.76 d−1, Ks = 972 mg/l COD, a = 0.58 mg O2/mg COD, b = 0.044 mg O2/mg MLVSS.d. The experimental findings were used in the design of a full-scale treatment system.

A systematic approach for model verification: application on seven published activated sludge models

2010 ◽  
Vol 61 (4) ◽  
pp. 825-839 ◽  
Author(s):  
H. Hauduc ◽  
L. Rieger ◽  
I. Takács ◽  
A. Héduit ◽  
P. A. Vanrolleghem ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Systematic Approach ◽  
Model Development ◽  
Model Verification ◽  
Software Implementation ◽  
Model Description ◽  
Kinetic Rate ◽  
Simulation Results ◽  
Activated Sludge Models

The quality of simulation results can be significantly affected by errors in the published model (typing, inconsistencies, gaps or conceptual errors) and/or in the underlying numerical model description. Seven of the most commonly used activated sludge models have been investigated to point out the typing errors, inconsistencies and gaps in the model publications: ASM1; ASM2d; ASM3; ASM3 + Bio-P; ASM2d + TUD; New General; UCTPHO+. A systematic approach to verify models by tracking typing errors and inconsistencies in model development and software implementation is proposed. Then, stoichiometry and kinetic rate expressions are checked for each model and the errors found are reported in detail. An attached spreadsheet (see http://www.iwaponline.com/wst/06104/0898.pdf) provides corrected matrices with the calculations of all stoichiometric coefficients for the discussed biokinetic models and gives an example of proper continuity checks.

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