DIGE-based identification of preferentially expressed proteins in early stage of lactogenic differentiation in buffalo (Bubalus bubalis) mammary epithelial cells

2021 ◽  
Vol 12 (1) ◽  
pp. 19-31
Author(s):  
Manoj K. Jena ◽  
Vijay Anand ◽  
Shalini Jaswal ◽  
Sudarshan Kumar ◽  
Thota. J. Reddy ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Early Stage ◽  
Bubalus Bubalis ◽  
Mammary Epithelial ◽  
Lactogenic Differentiation

Investigation of the role of microtubules in protein secretion from lactating mouse mammary epithelial cells

1992 ◽  
Vol 102 (2) ◽  
pp. 239-247 ◽  
Author(s):  
M.E. Rennison ◽  
S.E. Handel ◽  
C.J. Wilde ◽  
R.D. Burgoyne
Keyword(s):  
Plasma Membrane ◽  
Epithelial Cells ◽  
Protein Secretion ◽  
Secretory Pathway ◽  
Mammary Epithelial Cells ◽  
Early Stage ◽  
Major Effect ◽  
Vesicle Transport ◽  
Mammary Epithelial ◽  
Mammary Cells

Disruption of microtubules has been shown to reduce protein secretion from lactating mammary epithelial cells. To investigate the involvement of microtubules in the secretory pathway in these cells we have examined the effect of nocodazole on protein secretion from mammary epithelial cells derived from the lactating mouse. Mouse mammary cells have extensive microtubule networks and 85% of their tubulin was in a polymeric form. Treatment with 1 micrograms/ml nocodazole converted most of the tubulin into a soluble form. In a continuous labelling protocol it was found that nocodazole did not interfere with protein synthesis but over a 5 h period secretion was markedly inhibited. To determine whether the inhibition was at the level of early or late stages of the secretory pathway mammary cells were pulse-labelled for 1 h to label protein throughout the secretory pathway before nocodazole treatment. When secretion was subsequently assayed it was found to be slower and only partially inhibited. These findings suggest that the major effect of nocodazole is on an early stage of the secretory pathway and that microtubules normally facilitate vesicle transport to the plasma membrane. An involvement of microtubules in vesicle transport to the plasma membrane is consistent with an observed accumulation of casein vesicles in nocodazole-treated cells. Exocytosis stimulated by the calcium ionophore ionomycin was unaffected by nocodazole treatment. We conclude from these results that the major effect of nocodazole is at an early stage of the secretory pathway, one possible target being casein vesicle biogenesis in the trans-Golgi network.

Identification of suitable housekeeping genes for expression analysis in mammary epithelial cells of buffalo (Bubalus bubalis) during lactation cycle

2012 ◽  
Vol 147 (1-3) ◽  
pp. 72-76 ◽  
Author(s):  
Poonam Yadav ◽  
Desh Deepak Singh ◽  
Manishi Mukesh ◽  
R.S. Kataria ◽  
Anita Yadav ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Expression Analysis ◽  
Mammary Epithelial Cells ◽  
Housekeeping Genes ◽  
Bubalus Bubalis ◽  
Mammary Epithelial ◽  
Lactation Cycle

scholarly journals Spatiotemporal Dynamics of 3D Genome Architecture and Gene Expression during Lactogenic Differentiation of Murine Mammary Epithelial Cells

2017 ◽  
Vol 1 (Special Issue) ◽  
pp. 181-181
Author(s):  
Trinadha Rao Sornapudi ◽  
Prashanth Kumar Guthikonda ◽  
Srinivas Kethavath ◽  
Sailu Yellaboina ◽  
Sreenivasulu Kurukuti
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Spatiotemporal Dynamics ◽  
Mammary Epithelial ◽  
Genome Architecture ◽  
3D Genome ◽  
Lactogenic Differentiation

scholarly journals Correction: Impact of Heat Stress on Cellular and Transcriptional Adaptation of Mammary Epithelial Cells in Riverine Buffalo (Bubalus Bubalis)

PLoS ONE ◽  
2018 ◽  
Vol 13 (1) ◽  
pp. e0191380 ◽  
Author(s):  
Neha Kapila ◽  
Ankita Sharma ◽  
Amit Kishore ◽  
Monika Sodhi ◽  
Pawan K. Tripathi ◽  
...  
Keyword(s):  
Heat Stress ◽  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Bubalus Bubalis ◽  
Mammary Epithelial ◽  
Transcriptional Adaptation

scholarly journals Identification of Appropriate Reference Genes for qRT-PCR Analysis of Heat-Stressed Mammary Epithelial Cells in Riverine Buffaloes (Bubalus bubalis)

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Neha Kapila ◽  
Amit Kishore ◽  
Monika Sodhi ◽  
Ankita Sharma ◽  
Pawan Kumar ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Reference Genes ◽  
Mammary Epithelial Cells ◽  
Bubalus Bubalis ◽  
Mammary Epithelial ◽  
Pcr Analysis ◽  
Functional Classes ◽  
Gene Expression Studies ◽  
The Stability ◽  
Suitable Reference

Gene expression studies require appropriate normalization methods for proper evaluation of reference genes. To date, not many studies have been reported on the identification of suitable reference genes in buffaloes. The present study was undertaken to determine the panel of suitable reference genes in heat-stressed buffalo mammary epithelial cells (MECs). Briefly, MEC culture from buffalo mammary gland was exposed to 42 °C for one hour and subsequently allowed to recover at 37 °C for different time intervals (from 30 m to 48 h). Three different algorithms, geNorm, NormFinder, and BestKeeper softwares, were used to evaluate the stability of 16 potential reference genes from different functional classes. Our data identified RPL4, EEF1A1, and RPS23 genes to be the most appropriate reference genes that could be utilized for normalization of qPCR data in heat-stressed buffalo MECs.

scholarly journals Autocrine Growth Hormone Prevents Lactogenic Differentiation of Mouse Mammary Epithelial Cells

Endocrinology ◽  
2006 ◽  
Vol 147 (4) ◽  
pp. 1819-1829 ◽  
Author(s):  
Svetlana Mukhina ◽  
DongXu Liu ◽  
Ke Guo ◽  
Mireille Raccurt ◽  
Sahra Borges-Bendris ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Epithelial Cells ◽  
Mammary Epithelial Cell ◽  
Mammary Epithelial Cells ◽  
Three Dimensional ◽  
Late Pregnancy ◽  
Mammary Epithelial ◽  
Postnatal Ontogeny ◽  
Lactogenic Differentiation ◽  
Pregnancy And Lactation

We have examined the expression, postnatal ontogeny, and localization of mouse GH (mGH) and its relative expression during pregnancy, lactation, and weaning in the mouse. mGH mRNA and protein was expressed predominantly in the epithelial component of the mammary gland, and maximal expression was observed during the pubertal period. Autocrine mGH expression dramatically decreased during late pregnancy and lactation. Concordantly, autocrine mGH expression is repressed during forced differentiation of mouse HC11 mammary epithelial cells in culture. Forced expression of mGH in HC11 cells abrogated lactogenic differentiation as indicated by reduced expression of β-casein and reduced expression and loss of lateral epithelial localization of E-cadherin. Forced expression of mGH in mouse mammary epithelial cells increased cell survival and proliferation and consequently increased the size of mammary acinar-like structures formed in three-dimensional Matrigel. Thus, autocrine mGH expression in the mouse mammary epithelial cell is maximal at puberty and prevents mammary epithelial cell differentiation. Autocrine GH will therefore participate in mammary morphogenic processes at puberty.

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