CONSTRUCTION AND CHARACTERIZATION OF A NANOSTRUCTURED BIOCATALYST CONSISTING OF IMMOBILIZED LIPASE ON Mg-AMINO-CLAY

2021 ◽  
Author(s):  
Mingzhu Zhang ◽  
Shiyong Sun ◽  
Rui Lv ◽  
Yevgeny Aleksandrovich Golubev ◽  
Ke Wang ◽  
...  
Keyword(s):  
Immobilized Lipase

scholarly journals Production and Characterization of Biodiesel Using Nonedible Castor Oil by Immobilized Lipase fromBacillus aerius

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Sunil Kumar Narwal ◽  
Nitin Kumar Saun ◽  
Priyanka Dogra ◽  
Ghanshyam Chauhan ◽  
Reena Gupta
Keyword(s):  
Castor Oil ◽  
Immobilized Lipase ◽  
Chemical Reactor ◽  
Molar Ratio ◽  
Free System ◽  
H Nmr ◽  
Solvent Free System ◽  
Substrate Molar Ratio ◽  
Optimized Conditions

A novel thermotolerant lipase fromBacillus aeriuswas immobilized on inexpensive silica gel matrix. The immobilized lipase was used for the synthesis of biodiesel using castor oil as a substrate in a solvent free system at 55°C under shaking in a chemical reactor. Several crucial parameters affecting biodiesel yield such as incubation time, temperature, substrate molar ratio, and amount of lipase were optimized. Under the optimized conditions, the highest biodiesel yield was up to 78.13%. The characterization of synthesized biodiesel was done through FTIR spectroscopy,1H NMR spectra, and gas chromatography.

Characterization of cross-linked immobilized lipase from thermophilic mould Thermomyces lanuginosa using glutaraldehyde

2009 ◽  
Vol 100 (18) ◽  
pp. 4074-4076 ◽  
Author(s):  
Pritesh Gupta ◽  
Kakoli Dutt ◽  
Swati Misra ◽  
Shailendra Raghuwanshi ◽  
R.K. Saxena
Keyword(s):  
Immobilized Lipase

scholarly journals Production of Biodiesel Using Immobilized Lipase and the Characterization of Different Co-Immobilizing Agents and Immobilization Methods

2016 ◽  
Vol 8 (9) ◽  
pp. 764 ◽  
Author(s):  
Kang Zhao ◽  
Qinjian Di ◽  
Xi Cao ◽  
Meng Wang ◽  
Li Deng ◽  
...  
Keyword(s):  
Immobilized Lipase

scholarly journals Immobilization ofYarrowia lipolyticaLipase on Macroporous Resin Using Different Methods: Characterization of the Biocatalysts in Hydrolysis Reaction

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Jingjing Sun ◽  
Yiling Chen ◽  
Jun Sheng ◽  
Mi Sun
Keyword(s):  
Immobilized Lipase ◽  
Solvent Tolerance ◽  
Alkaline Ph ◽  
Lipase Hydrolysis ◽  
Organic System ◽  
Microbial Lipase ◽  
Application Range ◽  
Immobilized Lipases ◽  
Free Lipase

To improve the reusability and organic solvent tolerance of microbial lipase and expand the application of lipase (hydrolysis, esterification, and transesterification), we immobilized marine microbial lipase using different methods and determined the properties of immobilized lipases. Considering the activity and cost of immobilized lipase, the concentration of lipase was fixed at 2 mg/mL. The optimal temperature of immobilized lipases was 40°C and 5°C higher than free lipase. The activities of immobilized lipases were much higher than free lipase at alkaline pH (more than 50% at pH 12). The free lipase lost most activity (35.3%) and immobilized lipases retained more than 46.4% of their initial activity after 3 h heat treatment at 70°C. At alkaline pH, immobilized lipases were more stable than free lipase (more than 60% residue activity at pH 11 for 3 h). Immobilized lipases retained 80% of their activity after 5 cycles and increased enzyme activity (more than 108.7%) after 3 h treatment in tert-butanol. Immobilization of lipase which improved reusability of lipase and provided a chance to expand the application of marine microbial lipase in organic system expanded the application range of lipase to catalyze hydrolysis and esterification in harsh condition.

scholarly journals Enzymatic synthesis and characterization of polycaprolactone by using immobilized lipase onto a surface-modified renewable carrier

2016 ◽  
Vol 18 (3) ◽  
pp. 134-140 ◽  
Author(s):  
Cansu Ulker ◽  
Nurefsan Gokalp ◽  
Yuksel Guvenilir
Keyword(s):  
Rice Husk ◽  
Rice Husk Ash ◽  
Immobilized Lipase ◽  
Covalent Binding ◽  
Aqueous Media ◽  
Energy Saving Process ◽  
Surface Modified ◽  
First Time ◽  
Polymerization Conditions

Abstract In the present study, rice husk ash, which is a renewable and abundant material, was utilized as a carrier for lipase immobilization for the first time. Poly (ε-caprolactone) synthesis was successfully achieved by the new enzymatic catalyst: Candida antarctica lipase B immobilized onto surface-modified rice husk ashes by covalent binding. It was aimed to obtain optimum polymerization conditions at which highest molecular weight was reached and characterize the polymer produced. Moreover, thermal stability and effectiveness of the new biocatalyst in non-aqueous media were also shown with successful polymerization reactions. In addition, by using the new enzyme preparation, ε-caprolactone was able to be polymerized even at 30°C, which was promising for an energy saving process. Consequently, this work provides a new alternative route for poly (ε-caprolactone) synthesis.

Enzymatic and Chemical Synthesis and Characterization of Poly-6-O-(10-undecylenoyl) D-Glucose

2012 ◽  
Vol 550-553 ◽  
pp. 787-791
Author(s):  
Chao Wei ◽  
Yin Zhen Wang ◽  
Wei Yan ◽  
Ping Jia Yao ◽  
Yuan An Wei
Keyword(s):  
Nmr Spectroscopy ◽  
Molecular Sieves ◽  
Immobilized Lipase ◽  
13C Nmr Spectroscopy ◽  
Synthesis And Characterization ◽  
Undecylenic Acid ◽  
Optimal Conditions ◽  
Release Agent ◽  
O 10

Immobilized lipase Novozym-435 catalyzed regioselective synthesis of glucose and undecylenic acid(UA) to produce glucose mono-ester in Organic solvents was investigated. Reactionconditions were tested in order to produce the mono-ester more rapidly and in higher yield, the optimal conditions were obtained as: temperature at 50°C,mole ratio of glucose toUA of 1:2,concentration of molecular sieves and enzyme used as 0.56mg/mL and 10mg/mL respectively . After the reaction, product was purified, and characterized by MS and 13C NMR spectroscopy, and determined as 6-O-(10-undercylenoyl)-D-glucose, The product was used as the starting material, K2S2O8 as a chemical initiator, to polymerize and form the sugar-containing polymer, which could be used for medicine release agent and other application of biological materials.

Construction and characterization of a nanostructured biocatalyst consisting of immobilized lipase on aminopropyl-functionalized montmorillonite

2019 ◽  
Vol 183 ◽  
pp. 105329 ◽  
Author(s):  
Ke Wang ◽  
Shiyong Sun ◽  
Biaobiao Ma ◽  
Faqin Dong ◽  
Tingting Huo ◽  
...  
Keyword(s):  
Immobilized Lipase
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