scholarly journals Relationship Between Semen IL-6, IL-33 and Malondialdehyde Generation in Human Seminal Plasma and Spermatozoa

2021 ◽  
Author(s):  
Elena Moretti ◽  
Daniela Cerretani ◽  
Daria Noto ◽  
Cinzia Signorini ◽  
Francesca Iacoponi ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Semen Analysis ◽  
Sperm Parameters ◽  
Sperm Function ◽  
Human Semen ◽  
Who Guidelines ◽  
Human Seminal Plasma ◽  
Pathological Conditions ◽  
Number Of Patients ◽  
The Relationship

AbstractCytokines are physiological seminal components and their abnormal levels, reported in different pathological conditions, negatively influence the sperm function. We analysed the relationship between interleukin (IL)-6 and IL-33 levels and lipid peroxidation (LPO), measured both in semen and sperm lysate, in 44 human semen samples. The semen analysis was performed following the WHO guidelines. Seminal IL-6 and IL-33 concentrations were assessed by ELISA and LPO was evaluated measuring malondialdehyde (MDA) both in seminal plasma and viable spermatozoa. Two small groups of patients with varicocele and infection were extrapolated from the cases analysed and the variables compared with those of a group of control. IL-33 levels were undetectable in all samples and IL-6 levels were positively correlated with both seminal and sperm MDA concentrations (p < 0.01) and negatively with sperm parameters (p < 0.01). Seminal and sperm MDA levels were both negatively correlated with sperm parameters (p < 0.01). IL-6 and semen MDA showed an exponential positive relationship, whereas MDA values measured in viable spermatozoa were low until IL-6 amount reached a concentration of >30 pg/mL, rising consistently. By comparing the variables in the groups, we confirmed that a high IL-6 concentration in the varicocele and infection groups was concomitant with an increase of seminal MDA levels, but also with MDA measured in viable spermatozoa, which represents the novelty of this study. We identified the IL-6 threshold, beyond which sperm MDA concentration rises concomitantly with the increase of IL-6 concentration. Other studies are needed, considering the increasing number of patients with different pathologies affecting male infertility.

scholarly journals Anovel Immunological Technique for Identification of Human Seminal Fluid

2010 ◽  
Vol 7 (2) ◽  
pp. 1023-1027
Author(s):  
Baghdad Science Journal
Keyword(s):  
Seminal Plasma ◽  
Human Body ◽  
Seminal Fluid ◽  
Cross Reactivity ◽  
Body Fluids ◽  
Vaginal Fluid ◽  
Human Semen ◽  
Human Seminal Plasma ◽  
Ear Wax ◽  
Immunological Technique

An immunological technique was investigated for the detection of human semen in forensic analysis.This technique included a preparation of anti-human seminal plasma antibodies, by immunizing rabbits with treated human semen. The human semen was treated with an acid to prevent cross reactivity with other human body fluids. The antibody produced was tested against different animal,s seminal fluid samples (dog, goat ,sheep, cow) and human body fluids( saliva, blood , vaginal fluid, ear wax and human semen). It was found that using this developed technique was only selectively responsed with human semen . The prepered kit was evaluated and tested in Forensic laboratory- Ministry of Health. Finally, results were obtained in a comparison with the recommended techniques.

scholarly journals R-062. Total immunoreactive α-inhibin in human seminal plasma, sperm parameters and IVF rates

1997 ◽  
Vol 12 (Suppl_2) ◽  
pp. 263-263
Author(s):  
R. Matorras ◽  
J. Genolla ◽  
R. Mendoza ◽  
J. Uruena ◽  
M. Goitia ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Sperm Parameters ◽  
Human Seminal Plasma

scholarly journals A Comparative Study of Distribution of Protein and Cholesterol in Various Fractions of Human Semen from Infertile and Fertile Subjects

2012 ◽  
Vol 3 (3) ◽  
pp. 78-82 ◽  
Author(s):  
MS Srinivas ◽  
Vickram Sundaram ◽  
M Ramesh Pathy ◽  
TB Sridharan
Keyword(s):  
Molecular Weight ◽  
Comparative Study ◽  
Seminal Plasma ◽  
Semen Analysis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Human Semen ◽  
Exact Function ◽  
Wide Range ◽  
Significant Difference

ABSTRACT Aim To elucidate the concentration of the protein and cholesterol in different fractions of human semen from different infertile categories and comparing them with the fertile group. Materials and methods The human semen was collected from different infertile categories including oligoasthenospermia, asthenospermia, azoospermia, normospermia, oligospermia and fertile group. Immediately after collection, the semen analysis was done as per WHO standard protocols. After that, the semen was centrifuged to get the different fractions. Four main fractions were obtained, (1) spermatozoa, (2) debris or material that precipitates at 12 K rpm for 10 minutes, (3) prostasomes which was precipitated at 20K rpm for 120 minutes, (4) seminal plasma. The protein concentration was done by Lowry's method and cholesterol was estimated by diagnostic kit. Results Sodium dodecyl sulfate—polyacrylamide gel electrophoresis (SDS PAGE) was run for all the categories of semen samples for their seminal plasma and the fertility associated protein was identified. A significant difference was found in the concentration of proteins in all subfractions when compared between control and infertile categories. Almost 86% of the protein was recovered from soluble fraction. In case of azoospermia, the protein content was very low when compared with fertile group. Seminal plasma proteins were visualized by silver staining. The molecular weight of the protein bands were ranging from 6.5 to 205 kDa. The band with molecular weight around 55 kDa was found to be missing in case of oligoasthenospermia. This particular protein is said to be fertility associated protein. The content of cholesterol for different subfraction of the human semen samples from infertile and fertile samples was compared. A wide range of cholesterol was recovered from prostasomes, that too purified. Conclusion A thrive study have to be done in all the subfractions of the semen irrespective of the category of samples to know the exact function of the each subfractions in terms of protein and cholesterol distribution. How to cite this article Sundaram V, Srinivas MS, Rao KA, Pathy MR, Sridharan TB. A Comparative Study of Distribution of Protein and Cholesterol in Various Fractions of Human Semen from Infertile and Fertile Subjects. Int J Infertility Fetal Med 2012;3(3):78-82.

Fibrinogen-Like Substance and Thrombin-Like Enzyme in Human Seminal Plasma: Coagulation System of Human Semen

1997 ◽  
Vol 38 (1) ◽  
pp. 29-36 ◽  
Author(s):  
J.-Y. Park ◽  
Y. Yoshimura ◽  
S. Nozawa ◽  
T. Umeda ◽  
S. Akihama ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Coagulation System ◽  
Human Semen ◽  
Human Seminal Plasma ◽  
Plasma Coagulation

scholarly journals Analysis and Comparison of Zinc Micro Element Concentration Present in Human Seminal Plasma of Asthenospermia and Normospermia Conditions Using Atomic Absorption Spectroscopy for Male Infertility Diagnosis Related to Motility Issues

2021 ◽  
Vol 36 (1) ◽  
pp. 692-697
Author(s):  
Yaddala Thanusha ◽  
A.S. Vickram
Keyword(s):  
Atomic Absorption ◽  
Absorption Spectroscopy ◽  
Seminal Plasma ◽  
Atomic Absorption Spectroscopy ◽  
Zinc Concentration ◽  
Semen Analysis ◽  
Essential Element ◽  
World Health ◽  
Human Seminal Plasma ◽  
Zn Concentration

Aim: The major aim of this study is to compare Zinc (Zn) concentration present in human seminal plasma of asthenospermia (motility issues) and normospermia semen samples for identification of clinical relevance. Materials and Methods: Semen samples of normospermia (N=75), asthenospermia (N=75) were collected and semen analysis report has been done by standard world health organization protocol. Zn concentration was evaluated by using atomic absorption spectroscopy (AAS) for both the groups by using standard protocol. Results: Independent sample T-test on fertile and infertile men (asthenospermia and normospermia group) reveals that the zinc concentration is statistically insignificant (P=0.367). Zinc concentration was found to be high in normospermia (mean 2.5 mg/ml) over asthenospermia (mean 0.90 mg/ml) samples. Conclusion: Zn being an essential element for normal functioning for fertilization, its absence or less concentration may lead to infertility. We found high concentration of Zn in normospermia which reflects normal conditions for fertilization and less concentration in asthenospermia reflecting infertile conditions.

Levels of Cadmium, Chromium, Nickel, Manganese and Lead in Normal and Pathological Human Seminal Plasma

2005 ◽  
Vol 72 (4) ◽  
pp. 461-464 ◽  
Author(s):  
K. P. Skandhan ◽  
M. T. Makada ◽  
S. Amith
Keyword(s):  
Seminal Plasma ◽  
Statistical Difference ◽  
The Other ◽  
Human Seminal Plasma ◽  
Pathological Conditions ◽  
And Function ◽  
Nickel Manganese

In this study, we established the values for cadmium, chromium, nickel, manganese and lead in seminal plasma of normal and in different infertile conditions. Except for manganese, the other elements were not present in all normal samples. The value of nickel differed significantly from normal in all pathological conditions except azoospermia. Chromium showed a statistical difference in azoospermia when compared to that of normozoospermia. The presence of these elements in semen could have different roles to play in the survival and function of spermatozoa.

scholarly journals Impact of Seminal Plasma Trace Elements on Human Sperm Motility Parameters

2018 ◽  
Vol 56 (1) ◽  
pp. 15-20 ◽  
Author(s):  
Mohammad Mostakhdem Hashemi ◽  
Nasser Behnampour ◽  
Mojgan Nejabat ◽  
Afsaneh Tabandeh ◽  
Behrouz Ghazi-Moghaddam ◽  
...  
Keyword(s):  
Trace Elements ◽  
Sperm Motility ◽  
Seminal Plasma ◽  
Sperm Quality ◽  
Semen Analysis ◽  
Sperm Concentration ◽  
Negative Relationship ◽  
Human Sperm ◽  
Computer Assisted ◽  
Human Seminal Plasma

Abstract Introduction. Human seminal plasma contains a variety of macro and trace elements including magnesium (Mg), copper (Cu), zinc (Zn), and iron (Fe) that have essential roles in normal functioning of semen and its quality. The imbalance of these elements has been reported in several pathologic and male infertility disorders. Therefore, this study aimed to determine the levels of these elements in seminal plasma samples, their relationships with each other and their impact on sperm motility. Methods. Overall, 192 males (96 normospermic and 96 asthenospermic males) were enrolled in the study. Semen samples were collected by masturbation and computer-assisted/aided semen analysis of sperm motility was performed. The samples were centrifuged and seminal levels of Mg, Cu, Zn and Fe were measured using atomic absorption spectroscopy. Results. The levels of Zn did not differ between the two groups, while the levels of Mg, Cu, and Fe were significantly higher in normospermic males. Fe showed a positive correlation with Mg and Cu in asthenospermic group. However, a negative relationship was found between Mg and Fe levels and between Mg and sperm concentration in the normospermic group. Fe levels were higher in the normospermic group compared to the asthenospermic group. Nevertheless, increased Fe levels caused a decrease in most of sperm motility fractions. Conclusion: Elements play major roles in male fertility and directly affect sperm quality. According to the results of this study, the levels of Zn do not affect the sperm quality and motility, while Fe, Cu and Mg are decreased in males with sperm motility problems. Nevertheless, Fe levels can adversely affect sperm motility in normospermic men.

The heat production of human spermatozoa and seminal plasma; with comparative observations on bull semen

1960 ◽  
Vol 152 (948) ◽  
pp. 298-310 ◽  
Keyword(s):  
Seminal Plasma ◽  
Heat Production ◽  
Human Spermatozoa ◽  
Enzymatic Reactions ◽  
Human Semen ◽  
Human Seminal Plasma ◽  
Pronounced Difference ◽  
Bull Semen ◽  
Isothermal Calorimeter ◽  
Aerobic And Anaerobic

The aerobic and anaerobic heat production of human semen, spermatozoa and seminal plasma has been examined in an isothermal calorimeter, capacity 2.4 ml., working at atmospheric pressure. The heat production of bull semen, spermatozoa and seminal plasma was also measured, for comparison. The anaerobic heat production of human spermatozoa (in semen), per unit number of cells, is markedly higher than that of bull spermatozoa (in semen). The ratio may reach 4:1. Up to two-thirds of the heat production of human semen originates in the seminal plasma. The contribution of seminal plasma to the heat production of bull semen is insignificant. The anaerobic heat production of human seminal plasma is more than fifty times as great as that of bull seminal plasma. When anaerobic bull semen is aerated by mixing it in the calorimeter with an aerated diluent, there is a sudden burst of heat production. The same phenomenon occurs when human semen is mixed with an aerated diluent, though the effect is less marked. It is concluded that differences between the metabolism of human and bull spermatozoa are quantitative and not qualitative. (In the past, human spermatozoa have sometimes been said to be unique in being unable to respire, in spite of containing cytochrome.) The pronounced difference between the aerobic and anaerobic heat production of bull and human seminal plasma is due to enzymatic reactions which take place in the latter. 23 mM-phosphate buffer, pH 7.4, inhibits the O 2 uptake of human spermatozoa.

Sign in / Sign up

Export Citation Format

Share Document