scholarly journals Anovel Immunological Technique for Identification of Human Seminal Fluid

2010 ◽  
Vol 7 (2) ◽  
pp. 1023-1027
Author(s):  
Baghdad Science Journal
Keyword(s):  
Seminal Plasma ◽  
Human Body ◽  
Seminal Fluid ◽  
Cross Reactivity ◽  
Body Fluids ◽  
Vaginal Fluid ◽  
Human Semen ◽  
Human Seminal Plasma ◽  
Ear Wax ◽  
Immunological Technique

An immunological technique was investigated for the detection of human semen in forensic analysis.This technique included a preparation of anti-human seminal plasma antibodies, by immunizing rabbits with treated human semen. The human semen was treated with an acid to prevent cross reactivity with other human body fluids. The antibody produced was tested against different animal,s seminal fluid samples (dog, goat ,sheep, cow) and human body fluids( saliva, blood , vaginal fluid, ear wax and human semen). It was found that using this developed technique was only selectively responsed with human semen . The prepered kit was evaluated and tested in Forensic laboratory- Ministry of Health. Finally, results were obtained in a comparison with the recommended techniques.

421. Seminal fluid TGFβ regulates follistatin mRNA expression human Ect1 cervical epithelial cells

2008 ◽  
Vol 20 (9) ◽  
pp. 101 ◽  
Author(s):  
D. J. Sharkey ◽  
S. A. Robertson
Keyword(s):  
Epithelial Cells ◽  
Inflammatory Response ◽  
Mrna Expression ◽  
Seminal Plasma ◽  
Seminal Fluid ◽  
Differentially Expressed Gene ◽  
Female Reproductive Tract ◽  
High Concentration ◽  
Human Seminal Plasma ◽  
Local Inflammatory Response

Introduction of seminal fluid into the female reproductive tract following coitus stimulates a local inflammatory response. Inflammatory leukocyte recruitment is regulated by induction of cytokine and chemokine synthesis in female tract epithelial cells by seminal fluid signalling agents. Affymetrix microarray analysis in immortalised ectocervical epithelial (Ect1) cells identified the potent anti-inflammatory cytokine follistatin (FST) as the most strongly differentially expressed gene, with a ~12-fold increase in mRNA expression induced by seminal fluid. Follistatin has recently been implicated as a key cytokine in early pregnancy by studies in female follistatin null mice, which exhibit infertility as a consequence of failure to resolve the uterine post-mating inflammatory response. The aim of this study was to investigate seminal plasma regulation of follistatin in human Ect1 cervical cells, and to examine the role of the major active seminal fluid constituent, TGFβ, in controlling Ect1 cells follistatin mRNA expression. To confirm Affymetrix findings, qRT–PCR experiments were undertaken in Ect1 cells incubated with 10% pooled human seminal plasma (SP). Primers specific for the tissue bound isoform of follistatin (FST288) as well as both FST288 and the circulating 315 isoforms (FSTall) were used. Ect1 cell incubation with 10%SP elicited 3.8-fold and 4-fold increases in FST288 and FSTall respectively. Incubation of Ect1 cells with TGFβ1, TGFβ2 and TGFβ3 showed differential effects of the three isoforms, with rTGFβ2 inducing FST288 and FSTall, while rTGFβ1 and TGFβ3 exerted little effect.. These results suggest that seminal plasma induces follistatin synthesis after coitus and that TGFβ2 is at least partly responsible for this effect. Follistatin induced by seminal fluid may act to limit the course of inflammation after intercourse, and thereby prevent uncontrolled inflammatory damage. Follistatin induced in the female tissues would be augmented by follistatin delivered from the male, since human seminal plasma also contains a high concentration of this cytokine.

Fibrinogen-Like Substance and Thrombin-Like Enzyme in Human Seminal Plasma: Coagulation System of Human Semen

1997 ◽  
Vol 38 (1) ◽  
pp. 29-36 ◽  
Author(s):  
J.-Y. Park ◽  
Y. Yoshimura ◽  
S. Nozawa ◽  
T. Umeda ◽  
S. Akihama ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Coagulation System ◽  
Human Semen ◽  
Human Seminal Plasma ◽  
Plasma Coagulation

scholarly journals Correlations between Different Heavy Metals in Diverse Body Fluids: Studies of Human Semen Quality

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Lidia Mínguez-Alarcón ◽  
Jaime Mendiola ◽  
Manuela Roca ◽  
José J. López-Espín ◽  
José J. Guillén ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Seminal Plasma ◽  
Semen Quality ◽  
Biological Fluids ◽  
Body Fluids ◽  
Male Reproduction ◽  
World Health ◽  
Semen Parameters ◽  
Human Semen ◽  
Health Organization

It has been hypothesized that exposure to heavy metals may impair male reproduction. To measure the effect produced by low doses of heavy metals on semen parameters, it is necessary to clarify in which body fluids those measurements must be performed. Sixty-one men attending infertility clinics participated in our study. Concentrations of lead, cadmium, and mercury were measured in whole blood, blood plasma, and seminal plasma using spectroanalytical and electrochemical methods. Semen analyses were performed according to World Health Organization criteria. For statistical analysis, Spearman's rank correlations, mean comparison tests, and discriminant analysis were calculated. Significant correlations between the measured concentrations of the three heavy metals in the same biological fluids were observed. However, no similar relationship was seen when comparing the concentrations in different body fluids of the same metal. According to our results and previous publications, seminal plasma might be the best body fluid for assessing impairment of human semen parameters.

The heat production of human spermatozoa and seminal plasma; with comparative observations on bull semen

1960 ◽  
Vol 152 (948) ◽  
pp. 298-310 ◽  
Keyword(s):  
Seminal Plasma ◽  
Heat Production ◽  
Human Spermatozoa ◽  
Enzymatic Reactions ◽  
Human Semen ◽  
Human Seminal Plasma ◽  
Pronounced Difference ◽  
Bull Semen ◽  
Isothermal Calorimeter ◽  
Aerobic And Anaerobic

The aerobic and anaerobic heat production of human semen, spermatozoa and seminal plasma has been examined in an isothermal calorimeter, capacity 2.4 ml., working at atmospheric pressure. The heat production of bull semen, spermatozoa and seminal plasma was also measured, for comparison. The anaerobic heat production of human spermatozoa (in semen), per unit number of cells, is markedly higher than that of bull spermatozoa (in semen). The ratio may reach 4:1. Up to two-thirds of the heat production of human semen originates in the seminal plasma. The contribution of seminal plasma to the heat production of bull semen is insignificant. The anaerobic heat production of human seminal plasma is more than fifty times as great as that of bull seminal plasma. When anaerobic bull semen is aerated by mixing it in the calorimeter with an aerated diluent, there is a sudden burst of heat production. The same phenomenon occurs when human semen is mixed with an aerated diluent, though the effect is less marked. It is concluded that differences between the metabolism of human and bull spermatozoa are quantitative and not qualitative. (In the past, human spermatozoa have sometimes been said to be unique in being unable to respire, in spite of containing cytochrome.) The pronounced difference between the aerobic and anaerobic heat production of bull and human seminal plasma is due to enzymatic reactions which take place in the latter. 23 mM-phosphate buffer, pH 7.4, inhibits the O 2 uptake of human spermatozoa.

scholarly journals THE CONCENTRATION OF INTERLEUKIN-6 AND INTERLEUKIN-8 IN HUMAN SEMEN WITH HIGH VISCOSITY

2021 ◽  
pp. 29-39
Author(s):  
D. Y. Sosnin ◽  
K. R. Gal'kovich ◽  
A. V. Krivtsov
Keyword(s):  
Seminal Plasma ◽  
Interleukin 6 ◽  
Comparison Group ◽  
Large Range ◽  
High Viscosity ◽  
Main Group ◽  
Interleukin 8 ◽  
Human Semen ◽  
Human Seminal Plasma ◽  
Range Of Values

Objective: to estimate the effect of ejaculate consistency on the levels of interleukin-6 and interleukin-8 in human seminal plasma. Material and methods. The concentration of interleukin-6 and interleukin-8 was determined by ELISA using the kit manufactured by «Vector-Best» (Russia). The study included 64 men: the main group (n = 30) presents patients with high semen viscosity, the comparison group (n = 34) presents men with normal semen viscosity. Results. In average, interleukin-6 level in the semen was 13.45 pg/ml, the median was 13.79 pg/ml; the data ranged from 8.24 pg/ml to 19.34 pg/ml. In average, level of interleukin-8 was 28.9 pg/ml, the median – 13.96 pg/ml; there is a large range of values from 0.202 pg/ml to 174.5 pg/ml. There are no significant differences in the values of interleukin-6 and interleukin-8 of the main group from the comparison group: for interleukin-6, U = 377.0 (p = 0.074655); for interleukin-8, U = 407.0 (p = 0.863852). The data obtained did not correlate neither between groups nor with the fertility markers of the human semen.<br>Conclusion. Interleukin-6 and interleukin-8 levels in the human seminal plasma do not depend on semen viscosity.

scholarly journals Development of a Protein Microarray Chip with Enhanced Fluorescence for Identification of Semen and Vaginal Fluid

Sensors ◽  
2018 ◽  
Vol 18 (11) ◽  
pp. 3874 ◽  
Author(s):  
Naseem Abbas ◽  
Xun Lu ◽  
Mohsin Ali Badshah ◽  
Jung Bin In ◽  
Won Il Heo ◽  
...  
Keyword(s):  
Incubation Time ◽  
Limit Of Detection ◽  
Protein Microarray ◽  
Cross Reactivity ◽  
Body Fluids ◽  
Identification System ◽  
Vaginal Fluid ◽  
Fluorescence Signal ◽  
Enhanced Fluorescence ◽  
Microarray Chip

The detection of body fluids has been used to identify a suspect and build a criminal case. As the amount of evidence collected at a crime site is limited, a multiplex identification system for body fluids using a small amount of sample is required. In this study, we proposed a multiplex detection platform using an Ag vertical nanorod metal enhanced fluorescence (MEF) substrate for semen and vaginal fluid (VF), which are important evidence in cases of sexual crime. The Ag nanorod MEF substrate with a length of 500 nm was fabricated by glancing angle deposition, and amino functionalization was conducted to improve binding ability. The effect of incubation time was analyzed, and an incubation time of 60 min was selected, at which the fluorescence signal was saturated. To assess the performance of the developed identification chip, the identification of semen and VF was carried out. The developed sensor could selectively identify semen and VF without any cross-reactivity. The limit of detection of the fabricated microarray chip was 10 times better than the commercially available rapid stain identification (RSID) Semen kit.

Proteins of human semen. I. Two-dimensional mapping of human seminal fluid.

1981 ◽  
Vol 27 (8) ◽  
pp. 1335-1340 ◽  
Author(s):  
J J Edwards ◽  
S L Tollaksen ◽  
N G Anderson
Keyword(s):  
Seminal Plasma ◽  
Serum Proteins ◽  
Seminal Fluid ◽  
Ethylenediaminetetraacetic Acid ◽  
Prostatic Acid Phosphatase ◽  
Two Dimensional ◽  
Human Seminal Plasma ◽  
Preparation Methods ◽  
Electrophoretic Transfer ◽  
Dimensional Mapping

Abstract The proteins in human seminal plasma were mapped by high-resolution two-dimensional electrophoresis (ISO-DALT and BASO-DALT systems). When analyzed under dissociating conditions, samples from normal fertile males revealed a pattern of over 200 proteins, ranging in mass from 10 000 to 100 000 daltons. Comparison of the mapped proteins from these males and those who had undergone vasectomy allowed us to identify one series of glycoproteins as missing from the semen from vasectomized individuals. Glycoproteins isolated by affinity chromatography with use of concanavalin A were also mapped. Some of the protein spots were identified either by co-electrophoresis with purified proteins or by the electrophoretic transfer of proteins to nitrocellulose sheets and subsequent detection by immunological procedures. The proteins identified include a number of serum proteins as well as prostatic acid phosphatase and creatine kinase. Proteolytic events shown to occur during the liquefaction of semen that occurs early after collection indicate the importance of carefully controlled collection and preparation methods for clinical evaluation of seminal plasma. Ethylenediaminetetraacetic acid and phenylmethylsulfonyl fluoride inhibit this proteolysis.

scholarly journals The High Content of Fructose in Human Semen Competitively Inhibits Broad and Potent Antivirals That Target High-Mannose Glycans

2020 ◽  
Vol 94 (9) ◽  
Author(s):  
Jacklyn Johnson ◽  
Manuel G. Flores ◽  
John Rosa ◽  
Changze Han ◽  
Alicia M. Salvi ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Seminal Fluid ◽  
Cell Attachment ◽  
Sexual Transmission ◽  
Antiviral Agents ◽  
High Concentration ◽  
Human Seminal Plasma ◽  
Sexual Transmission Of Hiv ◽  
Hiv 1

ABSTRACT Semen is the primary transmission vehicle for various pathogenic viruses. Initial steps of transmission, including cell attachment and entry, likely occur in the presence of semen. However, the unstable nature of human seminal plasma and its toxic effects on cells in culture limit the ability to study in vitro virus infection and inhibition in this medium. We found that whole semen significantly reduces the potency of antibodies and microbicides that target glycans on the envelope glycoproteins (Envs) of HIV-1. The extraordinarily high concentration of the monosaccharide fructose in semen contributes significantly to the effect by competitively inhibiting the binding of ligands to α1,2-linked mannose residues on Env. Infection and inhibition in whole human seminal plasma are accurately mimicked by a stable synthetic simulant of seminal fluid that we formulated. Our findings indicate that, in addition to the protein content of biological secretions, their small-solute composition impacts the potency of antiviral microbicides and mucosal antibodies. IMPORTANCE Biological secretions allow viruses to spread between individuals. Each type of secretion has a unique composition of proteins, salts, and sugars, which can affect the infectivity potential of the virus and inhibition of this process. Here, we describe HIV-1 infection and inhibition in whole human seminal plasma and a synthetic simulant that we formulated. We discovered that the sugar fructose in semen decreases the activity of a broad and potent class of antiviral agents that target mannose sugars on the envelope protein of HIV-1. This effect of semen fructose likely reduces the efficacy of such inhibitors to prevent the sexual transmission of HIV-1. Our findings suggest that the preclinical evaluation of microbicides and vaccine-elicited antibodies will be improved by their in vitro assessment in synthetic formulations that simulate the effects of semen on HIV-1 infection and inhibition.

scholarly journals A Proposed Procedure for Discriminating between Nasal Secretion and Saliva by RT-qPCR

Diagnostics ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 519
Author(s):  
Tomoko Akutsu ◽  
Ken Watanabe
Keyword(s):  
Sexual Assault ◽  
Cross Reactivity ◽  
Body Fluids ◽  
Nasal Secretion ◽  
Vaginal Fluid ◽  
Good Source ◽  
Expression Levels ◽  
Forensic Casework ◽  
Forensic Samples ◽  
Confirmatory Tests

In forensic casework, nasal secretion can be a good source of DNA. Moreover, saliva can prove useful in cases of sexual assault. However, discriminating between these body fluids is often difficult because of cross-reactivity between them on presumptive and confirmatory tests. Therefore, an RT-qPCR procedure was developed to discriminate between nasal secretion and saliva. Characteristic genes in nasal secretion and/or saliva (BPIFA1, STATH, HTN3, and PRH2) were selected as candidates. Discrimination criteria were established based on the expression levels of these markers in various body fluids. In addition, a flowchart was proposed and used to discriminate among nasal secretion, saliva, and other body fluids in various forensic samples. BPIFA1 was highly expressed in nasal secretion but was also expressed in saliva, semen, and vaginal fluid at trace levels. STATH was expressed in nasal secretion and saliva but not in other body fluids. HTN3 was specifically expressed in most of the saliva samples, as reported previously. Unexpectedly, PRH2 was expressed in only a few saliva samples. Using the proposed criteria and flowchart, nasal secretion and saliva were successfully discriminated among the various body fluids tested. The developed procedure could be useful in forensic casework.

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