Topography of age-related changes in corneal endothelial cell size. some preliminary microscopic observations

1984 ◽  
Vol 98 (4) ◽  
pp. 532
Author(s):  
SCHIMMELPFENNIG
Keyword(s):  
Endothelial Cell ◽  
Cell Size ◽  
Corneal Endothelial Cell ◽  
Age Related ◽  
Age Related Changes

scholarly journals An Observational Study of Corneal Endothelial Alterations in Eyes with Unilateral Pseudoexfoliation

2021 ◽  
Author(s):  
Archana Thool ◽  
Rashmi Ramani ◽  
Pravin Tidake
Keyword(s):  
Endothelial Cell ◽  
Observational Study ◽  
Cell Size ◽  
Cell Density ◽  
Cell Morphology ◽  
Coefficient Of Variation ◽  
Anterior Segment ◽  
Corneal Endothelial Cell ◽  
P Value ◽  
Corneal Endothelial Cell Density

Introduction: Pseudoexfoliation (PXF) is an age-related generalised disorder of extracellular matrix primarily affecting eyes. It is characterised by deposition of pseudoexfoliative material in the anterior segment of eye. Also it is a bilateral condition but can have asymmetric presentation and can be clinically seen only in one eye. Alteration in endothelial cell morphology along with other alteration in anterior segment of the eye can compromise the surgical outcome. Aim: To evaluate the corneal endothelial cell density and morphology in patients with unilateral PXF. Materials and Methods: The present study was hospital-based cross-sectional observational study which included 55 patients with unilateral PXF, who came to the Department of Opthalmology, Acharya Vinoba Bhave Rural Hospital (AVBRH), Wardha, Maharashtra, India between June 2020 to November 2020. Specular microscopy was performed on all eyes to evaluate cell density and morphology, coefficient of variation in cell size and percentage of hexagonal cells in corneal endothelium and compared with fellow normal eye. Statistical analysis was done by using descriptive and inferential statistics using students unpaired t-test, p-value <0.05 was considered as level of significance. Results: The present study included 55 patients, of which 26 were males and 29 were females. Mean age of the patients was 63.85±7.05 years. Majority of patients were in the range of 61 to 70 years (56.36%). The mean corneal endothelial cell density in the PXF eye was 2299.54±84.95 cells/mm2, which was significantly lower as compared to fellow normal eye with p-value <0.001. Mean coefficient of variation of cell size in PXF eye was 35.49±4.62, p-value=0.064 and hexagonality was 51.12±4.91, p-value=0.13. Both the parameters were statistically non significant on comparison of two eyes. Central corneal thickness was thicker in pseudoexfoliative eye of 551.29±33.11 microns (μm), than fellow eye but was statistically non significant. Conclusion: Eyes with PXF had alteration in endothelial cell morphology with decrease in cell density as compared to eyes without PXF clinically.

scholarly journals Corneal Endothelial Cell Density and Morphology in Healthy Turkish Eyes

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Ceyhun Arıcı ◽  
Osman Sevki Arslan ◽  
Funda Dikkaya
Keyword(s):  
Endothelial Cell ◽  
Cell Size ◽  
Cell Density ◽  
Central Corneal Thickness ◽  
Corneal Thickness ◽  
Corneal Endothelial Cell ◽  
Endothelial Cell Density ◽  
Japanese American ◽  
Significant Difference ◽  
Corneal Endothelial Cell Density

Purpose. To describe the normative values of corneal endothelial cell density, morphology, and central corneal thickness in healthy Turkish eyes.Methods. Specular microscopy was performed in 252 eyes of 126 healthy volunteers (M : F, 42 : 84). Parameters studied included mean endothelial cell density (MCD), mean cell area (MCA), coefficient of variation (CV) in cell size, percentage of hexagonal cells, and central corneal thickness (CCT).Results. The mean age of volunteers was44.3±13.5(range, 20 to 70) years. There was a statistically significant decrease in MCD (P<0.001; correlation, −0.388) and percentage of hexagonal cells, (P<0.001; correlation, −0.199) with age. There was also a statistically significant increase in MCA (P<0.001; correlation, 0.363) with increasing age. There was no statistically significant difference in MCD, MCA, CV in cell size, percentage of hexagonal cells, and CCT between genders and there was also no significant difference in these parameters between fellow eyes of subjects.Conclusions. Normotive data for the endothelium in the Turkish population are reported. Endothelial cell density in the Turkish eyes is less than that described in the Japanese, American, Chinese, and Filipino eyes and higher than that described in Indian, Thai, and Iranian eyes.

The state of corneal endothelial cells in the dynamics of surgical treatment of age-related and diabetic cataracts

2021 ◽  
pp. 47-50
Author(s):  
S.Y. Totskova ◽  
◽  
A.E. Babushkin ◽  
Е.M. Garipova ◽  
◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Surgical Treatment ◽  
Corneal Endothelium ◽  
Corneal Endothelial Cell ◽  
Corneal Endothelial Cells ◽  
Diabetic Cataract ◽  
Age Related ◽  
Corneal Endothelial Cell Density

Purpose. To assess the state of the corneal endothelium in patients with diabetic and immature age - related cataracts before and in the long term after phacoemulsification. Material and methods. There were 25 patients (43 eyes) under observation in total. The average age is 66.4±10.1 years. There were 12 men and 13 women among the surveyed. The average time after cataract phacoemulsification was 5.6±1.7 years. There were 2 groups of patients: group I (main) included 10 patients (16 eyes) with diabetic cataract, group 2 (control) consisted of 15 patients (27 eyes) with immature senile cataract (ISC) without diabetes. Before and after phacoemulsification the central corneal thickness (CCT), as well as polymegatism, pleomorphism, and corneal endothelial cell density (ECD) were studied using an EM-2000 endothelial microscope (Nidek, Japan). Results. In the long-term period after cataract phacoemulsification there was decreasing trend in the corneal endothelial cell density (ECD) as in patients operated on for immature senile and especially diabetic cataracts (by 1.5 times). The initial data of ECD in patients with diabetic cataract in comparison with ISC were lower although not significantly. There was also a decreasing trend in the percentage of hexaganality and the number of normal endothelial cells, as well as an increase in the coefficient of their polymegatism after phacoemulsification in patients with DM in comparison with patients without it. Conclusion. The study showed that phacoemulsification of diabetic cataracts is associated with more pronounced damage to the corneal endothelium when compared with the ISC group. During phacoemulsification with IOL implantation, especially in patients with diabetic cataracts, you should pay attention to the initially possible smaller ECD and take this circumstance into account when performing phacoemulsification. Key words: diabetic cataract, surgical treatment, phacoemulsification, endothelial microscopy, corneal endothelial cells.

scholarly journals The Effects of Metformin on Age-Related Changes in the Liver Sinusoidal Endothelial Cell

2019 ◽  
Author(s):  
Nicholas J Hunt ◽  
Glen P Lockwood ◽  
Sun Woo (Sophie) Kang ◽  
Tamara Pulpitel ◽  
Ximonie Clark ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Insulin Resistance ◽  
Endothelial Cell ◽  
Old Age ◽  
Sinusoidal Endothelial Cell ◽  
Liver Sinusoidal Endothelial Cell ◽  
Age Related ◽  
Sinusoidal Endothelium ◽  
Endothelial Nitric Oxide ◽  
Age Related Changes

Abstract Age-related changes in the liver sinusoidal endothelium, particularly the reduction in fenestrations, contribute to insulin resistance in old age. Metformin impacts on the aging process and improves insulin resistance. Therefore, the effects of metformin on the liver sinusoidal endothelium were studied. Metformin increased fenestrations in liver sinusoidal endothelial cells isolated from both young and old mice. Mice administered metformin in the diet for 12 months had increased fenestrations and this was associated with lower insulin levels. The effect of metformin on fenestrations was blocked by inhibitors of AMP-activated protein kinase (AMPK), endothelial nitric oxide synthase, and myosin light chain kinase phosphorylation. Metformin led to increased transgelin expression and structural changes in the actin cytoskeleton but had no effect on lactate production. Metformin also generated fenestration-like structures in SK-Hep1 cells, a liver endothelial cell line, and this was associated with increased ATP, cGMP, and mitochondrial activity. In conclusion, metformin ameliorates age-related changes in the liver sinusoidal endothelial cell via AMPK and endothelial nitric oxide pathways, which might promote insulin sensitivity in the liver, particularly in old age.

scholarly journals Profile of central corneal thickness and corneal endothelial cell morpho-density of in healthy Congolese eyes

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Joseph-Theodore K. Kelekele ◽  
David L. Kayembe ◽  
Jean-Claude Mwanza
Keyword(s):  
Endothelial Cell ◽  
Cell Size ◽  
Central Corneal Thickness ◽  
Corneal Thickness ◽  
Corneal Endothelial Cell ◽  
Normative Values ◽  
Specular Microscopy ◽  
Reference Limits ◽  
Systemic Factors ◽  
Corneal Endothelial Cell Density

Abstract Purpose To determine the pachymetric and corneal endothelial cell morphometric features and their relationship to ocular and systemic factors in healthy Congolese subjects. Methods Non-contact specular microscopy was used in 278 healthy eyes (278 subjects) to measure central corneal thickness (CCT), corneal endothelial cell density (CECD) along with cell size, coefficient of variation (CV) in cell size, and hexagonality (HEX). The lower and upper reference limits and average values for each parameter were determined. Correlation and association of average values with anthropo-demographic and clinical variables were assessed. Results The mean age was 38.9 ± 17.2 years (10.9–80.7 years). Average values were 504.2 ± 30.7 μm (CCT), 2907.1 ± 290.9 cells/mm2 (CECD), 348.5 ± 38.4 μm2 (cell size), 32.9 ± 3.6% (CV), and 51.8 ± 7.2% (HEX). CCT was 504.9 ± 33.6 μm in men and 503.6 ± 28.3 μm in women (p = .73); values for CECD were 2917.1 ± 253.5 cells/mm2 and 2899.2 ± 317.8 cells/mm2 (p = 0.61), respectively. Lower and upper reference limits were 449.6 μm and 566.0 μm for CCT, and 2165.3 cells/mm2 and 3414.4 cells/mm2 for CECD, respectively. CCT correlated with body mass index (BMI), (r = − 0.12, P = 0.04). CECD decreased with age (r = − 0.49, P < 0.001), BMI (r = − 0.20, P = 0.001), intraocular pressure (r = − 0.13, P = 0.029) and ocular perfusion pressure (r = − 0.28, P = 0.028). CECD decayed by 8.3 cells/mm2 or 0.30% per year of age and CCT decreased by 0.72 μm per kg/m2. Conclusions Mean central cornea was thinner, CECD higher, and references limits lower than reported in other African populations. The CCT and CECD normative values reported herein will be useful for both clinical and research purposes in this population.

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