Peroxidatic activity of metalloporphyrin binding to serum albumin: Enhancement effect of serum albumin on metalloporphyrin catalyzed luminol chemiluminescence reaction

1995 ◽  
Vol 300 (1-3) ◽  
pp. 215-220 ◽  
Author(s):  
Tie Jian-Ke ◽  
Chang Wen-Bao ◽  
Ci Yun-Xiang
Keyword(s):  
Serum Albumin ◽  
Enhancement Effect ◽  
Luminol Chemiluminescence ◽  
Peroxidatic Activity

scholarly journals Enhancement of Anti-allergic Effect of Diethylcarbamazine Citrate in Asthmatic Mouse Model: Testing of Anti-drug Antibodies and Querceti

2020 ◽  
Author(s):  
Nosibah Abdul-Razek ◽  
Al-Mahy M. El-Mallah ◽  
Abdelaziz S. A Abuelsaad ◽  
Mahmoud Abdel-Latif
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Lung Tissue ◽  
Bovine Serum ◽  
Passive Immunization ◽  
Enhancement Effect ◽  
Alveolar Wall ◽  
Leukocyte Infiltration ◽  
Diethylcarbamazine Citrate ◽  
Allergic Effect

Diethylcarbamazine citrate (DEC) is known as an effective treatment for bronchial asthma because of its ability to reduce eosinophil trafficking to the lung tissue. The current study aimed to potentiate the anti-allergic effect of the drug by passive immunization of the asthmatic model with anti-DEC antibody or prior treatment with quercetin (Qur). Eight mice groups were categorized into control, the model of lung asthma, treated with DEC, passively immunized with anti(α)-bovine serum albumin Ab, anti-DEC Ab, prior exposure to 10, 20, or 40 mg Qur/Kg. b.wt. Both eosinophil peroxidase (EPO) and eotaxin2 in the lung tissues were performed. Serum levels of cytokines, bronchoalveolar lavage fluid (BALF) IgE, rabbit anti-bovine serum albumin (anti-BSA), and DEC IgG in lung tissue homogenates were assayed by ELISA. Regarding the effect of anti-DEC Ab and Qur on DEC-induced recovery of histopathological alterations showed that the Ova group had peri-bronchial hyperplasia, mononuclear leukocyte infiltration, thickening in the wall of alveoli, and congested blood vessels. However, the reduction of inflammatory cells and thickened alveolar walls was dependent on the Qur dose. Qur40 enhanced the anti-allergic effect of DEC. Moreover, the present data revealed high levels of Th2 cytokines (IL-4 and IL-5) and IgE in the Ova group. An increased leukocyte infiltration/thickening of the alveolar wall and lung tissue EPO/eotaxin2 were also observed. Qur-40 could show an enhancement effect on DEC for the reduction of IL-4, IL-5, IgE, EPO, and eotaxin 2. Consequently, the IFN-γ/IL-4 ratio was increased. Qur at 40 mg/Kg could be recommended to enhance the DEC effect suggesting a novel approach for treatment.

scholarly journals Evaluation of the interactions between human serum albumin (HSA) and warfarin or diflunisal by using molecular fluorescence using two approaches

ADMET & DMPK ◽  
2018 ◽  
Vol 6 (1) ◽  
pp. 47 ◽  
Author(s):  
Susana Amézqueta ◽  
Anna Bolioli Maria Bolioli ◽  
José Luis Beltrán ◽  
Clara Ràfols
Keyword(s):  
Serum Albumin ◽  
Binding Site ◽  
Binding Constants ◽  
Drug Binding ◽  
Enhancement Effect ◽  
Antiinflammatory Drugs ◽  
Drug Binding Site ◽  
Star Program ◽  
Molecular Fluorescence ◽  
Double Logarithm

<p class="PaperH1">Serum albumin is the main drug transporter of the bloodstream and contains two main binding sites:  Sudlow I or acidic drug binding site, and Sudlow II or benzodiazepine binding site. Warfarin, a well-known anticoagulant drug commonly used in the prevention of thrombosis and thromboembolism, binds to Sudlow I site, whereas non-steroidal antiinflammatory drugs (NSAIDs) such as diflunisal bind preferentially to Sudlow II site.  Albumin is a fluorophore that modifies its fluorescence (quenching or enhancement effect) when it is bound to a drug. The application of the double logarithm Stern-Volmer equation allows the calculation of the stoichiometry and the binding constant of the process. This procedure does not consider the possible interferences coming from the fluorescence of the drug though. Another strategy to evaluate the binding constants is to consider the whole spectrum, taking into account all the possible species in equilibrium; in this case we have used an extended version of the STAR program, which can deal with 300 spectra, each containing up to 300 data points. The aim of this work is to compare both approaches to evaluate the interaction between warfarin (Sudlow I) and diflunisal (Sudlow II) and HSA: the double logarithm Stern-Volmer equation and the STAR program.</p>

Densitometric Identification of Primitive Erythroblasts After Reaction With Diaminobenzidine

1973 ◽  
Vol 31 ◽  
pp. 328-329
Author(s):  
John A. Trotter
Keyword(s):  
Red Blood Cells ◽  
Analytical Method ◽  
Blood Cells ◽  
Guinea Pigs ◽  
Specific Protein ◽  
Visual Examination ◽  
Hemoglobin Synthesis ◽  
Peroxidatic Activity ◽  
Small Density

Hemoglobin is the specific protein of red blood cells. Those cells in which hemoglobin synthesis is initiated are the earliest cells that can presently be considered to be committed to erythropoiesis. In order to identify such early cells electron microscopically, we have made use of the peroxidatic activity of hemoglobin by reacting the marrow of erythropoietically stimulated guinea pigs with diaminobenzidine (DAB). The reaction product appeared as a diffuse and amorphous electron opacity throughout the cytoplasm of reactive cells. The detection of small density increases of such a diffuse nature required an analytical method more sensitive and reliable than the visual examination of micrographs. A procedure was therefore devised for the evaluation of micrographs (negatives) with a densitometer (Weston Photographic Analyzer).

Biogenesis of Catalase-Positive Rods in Excretory Duct Cells of Salivary Glands

1976 ◽  
Vol 34 ◽  
pp. 62-63
Author(s):  
Dwight K. Romanovicz ◽  
Jacob S. Hanker
Keyword(s):  
Light Microscopy ◽  
Salivary Glands ◽  
Submandibular Gland ◽  
Excretory Duct ◽  
Exocrine Glands ◽  
Duct Cells ◽  
Peroxidatic Activity ◽  
Microscopic Studies ◽  
Different Dimensions

The presence of catalase-positive rods (Fig. 1) of different dimensions, which frequently have a crystalline appearance by light microscopy, has been reported. They seem to be related to peroxisomes which were characterized morphologically and cytochemically in parotid and other exocrine glands of the rat by Hand in 1973. Our light microscopic studies of these spherical microbodies and rods of different sizes, stained by virtue of the peroxidatic activity of their catalase, indicate that they are almost entirely confined to the cells of the striated and execretory ducts of the submandibular gland in the mouse. The rods were usually noted only in the proximity of the ductal microbodies. The latter frequently showed a tendency to appear in linear close array, or even to be contiguous (Fig. 2). This suggested that the rods could be formed by the fusion of microbodies.

Improvement of an artificial test substrate technique for evaluation of em immunocytochemical labeling

1987 ◽  
Vol 45 ◽  
pp. 762-763
Author(s):  
G. D. Gagne ◽  
M. F. Miller
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Artificial Substrate ◽  
Chemical Fixation ◽  
As If

We recently described an artificial substrate system which could be used to optimize labeling parameters in EM immunocytochemistry (ICC). The system utilizes blocks of glutaraldehyde polymerized bovine serum albumin (BSA) into which an antigen is incorporated by a soaking procedure. The resulting antigen impregnated blocks can then be fixed and embedded as if they are pieces of tissue and the effects of fixation, embedding and other parameters on the ability of incorporated antigen to be immunocyto-chemically labeled can then be assessed. In developing this system further, we discovered that the BSA substrate can also be dried and then sectioned for immunolabeling with or without prior chemical fixation and without exposing the antigen to embedding reagents. The effects of fixation and embedding protocols can thus be evaluated separately.

Children's Performance on a Binaural Fusion Task

1981 ◽  
Vol 24 (4) ◽  
pp. 520-525 ◽  
Author(s):  
Bruce L. Plakke ◽  
Daniel J. Orchik ◽  
Daniel S. Beasley
Keyword(s):  
Enhancement Effect ◽  
Sensation Level ◽  
Large Measure ◽  
Filter Bandwidth ◽  
Research Results ◽  
Presentation Modes ◽  
Monosyllabic Words ◽  
Better Than

Binaural auditory fusion of 108 children (4, 6, and 8 years old) was studied using three lists of monosyllabic words (WIPI) presented at two sensation levels (30 and 40 dB). The words were processed to produce three bandwidth conditions (100, 300, 600 Hz) and were administered via three presentation modes (binaural fusion 1, diotic, binaural fusion 2). Results showed improved discrimination scores with increasing age, sensation level, and filter bandwidth. Diotic scores were better than binaural fusion scores for the narrower bandwidth conditions, but the diotic enhancement effect was seriously compromised in the widest bandwidth (600 Hz) condition. The results confirmed the contention that prior research results were equivocal due, in large measure, to procedural variability. Methods for reducing such variability and enhancing the clinical viability of binaural fusion tasks are suggested.

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