An improved method for the production of Neurospora crassa spheroplasts and a new method for measuring transport in fragile cells

1974 ◽  
Vol 61 (2) ◽  
pp. 441-447 ◽  
Author(s):  
Gene A. Scarborough ◽  
Thomas H. Schulte
Keyword(s):  
Neurospora Crassa ◽  
New Method ◽  
Improved Method

Improved Method Based on Fluid Mechanics Analysis for Buoy Gauge Design and Experimental Research

2012 ◽  
Vol 163 ◽  
pp. 133-137
Author(s):  
Ao Yu Chen ◽  
Xu Dong Pan ◽  
Guang Lin Wang
Keyword(s):  
Fluid Mechanics ◽  
Experimental Research ◽  
Traditional Method ◽  
New Method ◽  
Improved Method ◽  
Advanced Method ◽  
Inner Surface ◽  
Mechanics Analysis ◽  
Linear Property

Traditional method of buoy gauge design is rather complicated, so an advanced method by building and solving fluid mechanics equations is proposed in this paper. The curve of the taper pipe inner surface is calculated, according to different buoy gravity and diameter. In order to examine the effect of this improved method, an experiment is carried out. Results show that linear property of the buoy gauge improved by new method is excellent.

A New Method for Aggregate Proportion Design of Mixture

2012 ◽  
Vol 178-181 ◽  
pp. 1250-1253
Author(s):  
Yu Hua Li ◽  
Kai Huang ◽  
Ying Zhen Gao
Keyword(s):  
Iterative Algorithm ◽  
Accurate Result ◽  
High Accuracy ◽  
New Method ◽  
Equation Method ◽  
Normal Equation ◽  
Improved Method ◽  
Mixture Ratio ◽  
Target Value ◽  
Initial Target

When we make design of mixture ratio, we always use normal equation method (NEM), but the solution value of that method may doesn’t meet our demand. When we use the new method that proposed in this paper, we can solve the problem. The new method makes an improvement for NEM. By iterative algorithm, the new method uses the gradation data calculated by NEM as initial target value, canceling the relevant aggregate if the result of proportion is negative, and setting the gradation to the boundary value if the target value goes beyond limits. According to the adjusted object value, using NEM again, the new aggregate proportion can be solved. Then the new gradation of mixture will come into being the next target value. Finally, the accurate result will meet our need. The new improved method has good engineering applicability and high accuracy

Transient Response of an Interface-Crack in a Layered Plate

1986 ◽  
Vol 53 (3) ◽  
pp. 579-586 ◽  
Author(s):  
T. Kundu
Keyword(s):  
Stress Field ◽  
Interface Crack ◽  
Transient Response ◽  
Singular Integrals ◽  
Fortran Program ◽  
Numerical Results ◽  
New Method ◽  
Layered Plate ◽  
Improved Method ◽  
Sample Problem

In this paper, the transient response of an interface crack, in a two layered plate subjected to an antiplane stress field, is analytically computed. The problem is formulated in terms of semi-infinite integrals following the technique developed by Neerhoff (1979). It has been shown that the major steps of Neerhoff’s technique, which was originally developed for layered half-spaces, can also be applied to layered plate problems. An improved method for manipulation of semi-infinite singular integrals is also presented here. Finally, the new method is coded in FORTRAN program and numerical results for a sample problem are presented.

scholarly journals An improved method for preparation of mouse retinal cryosections

2020 ◽  
Vol 64 (3) ◽  
Author(s):  
Ling Li ◽  
Yongqiong Lin ◽  
Fang Yang ◽  
Tongdan Zou ◽  
Jialiang Yang ◽  
...  
Keyword(s):  
Retinal Detachment ◽  
Vision Research ◽  
New Method ◽  
Specific Marker ◽  
Improved Method ◽  
High Quality ◽  
New Methods ◽  
Modified Method ◽  
Routine Assay ◽  
Super Glue

Immunohistochemistry using mouse retinal cryosections is a routine assay used in vision research. However, retinal tissues are fragile, and it is difficult to obtain an ideal retinal cryosection. Here, we developed a modified method for preparing retinal cryosection. Super Glue was applied on the surface of the sclera before the cornea and the lens are removed from either the unfixed or PFA-fixed mouse eyeballs. The new methods largely prevented retinal detachment in mouse retinal cryosections. Immunostaining of retinal cryosections derived from PFA-fixed mouse eyes using rod and cone markers yielded high-quality immunofluorescent images. Immunolabeling of retinal cryosections obtained from unfixed mouse eyes using a cilium-specific marker had improved orientations of photoreceptor connecting cilia. This new method substantially improves the morphology and immunostaining results of fixed and unfixed mouse eyes.

Collaborative Study of an Improved Method for the Enumeration and Confirmation of Clostridium perfringens in Foods

1976 ◽  
Vol 59 (3) ◽  
pp. 606-612
Author(s):  
Stanley M Harmon
Keyword(s):  
Clostridium Perfringens ◽  
Collaborative Study ◽  
Egg Yolk ◽  
New Method ◽  
Improved Method ◽  
Nitrate Medium ◽  
Vegetative Cells ◽  
Roast Beef

Abstract A collaborative study was conducted in 10 laboratories to evaluate the performance of a new method for the enumeration of vegetative cells of Clostridium perfringens in foods. Results obtained by the new method were compared with results from the official first action method, 46.049–46.053. Per cent recoveries of 4 C. perfringens strains from inoculated roast beef samples were higher and more consistent in tryptose-sulfite-cycloserine (TSC) agar with or without added egg yolk than in sulfitepolymyxin-sulfadiazine (SPS) agar, specified in the official first action method. The confirmatory technique utilized in the new method was also found to be more reliable than the technique described in the official first action method. Based on the collaborative results, the new method with TSC agar for enumeration and a modified motility-nitrate medium together with a lactose-gelatin medium for confirmation of C. perfringens has been adopted as official first action to replace 46.049–46.053.

A New Method for the Biomimetic Synthesis of 2-Hydroxy-3-Amino-1,4-Naphthoquinone

2013 ◽  
Vol 781-784 ◽  
pp. 287-290 ◽  
Author(s):  
Rui Liu ◽  
Hong Li ◽  
Wen Yan Ma
Keyword(s):  
Aqueous Medium ◽  
Chemical Structure ◽  
Stannous Chloride ◽  
New Method ◽  
Biomimetic Synthesis ◽  
Hydroxyl Group ◽  
Amino Group ◽  
Improved Method ◽  
Moderate Yield

Methodology has been sought towards 1,4-naphthoquinone bearing amino group and hydroxyl group in the quinonoid ring. In the first of these objectives, 2-Hydroxy-3-nitro-1,4-naphthoquinone was synthesized by two kinds of improved method giving moderate yield. Then treatment of 2-hydroxy-3-nitro-1,4-naphthoquinone with stannous chloride in aqueous medium afforded 2-hydroxy-3-amino-1,4-naphthoquinone. The chemical structure of the product obtained by the process of the present study was verified by IR and NMR.

An improved method for determining the nitrogen solubility of caseinates

1990 ◽  
Vol 57 (2) ◽  
pp. 207-212
Author(s):  
Wolfgang Kneifel ◽  
Anne Beurel
Keyword(s):  
Filter Paper ◽  
Nitrogen Solubility ◽  
New Method ◽  
Nitrogen Solubility Index ◽  
Improved Method

SummaryThe nitrogen solubility index (NSI) method for the examination of caseinates was evaluated. It was found that widely scattering NSI values are mainly due to ineffective centrifugation and filtration of the caseinate dispersion. In order to avoid this, an improved combined centrifugation–filtration procedure was developed as an alternative to the original NSI method, using an Amicon micropartition system MPS-1 equipped with discs of filter paper. The micropartition devices can be centrifuged in a Gerber centrifuge of the type used for butyromctric fat determination. Several types of caseinates were evaluated with the new method and yielded a markedly smaller range of single values than were obtained with the NSI method.

An improved method for recovering and preconcentrating mercury in natural water samples for stable isotope analysis

2019 ◽  
Vol 34 (11) ◽  
pp. 2303-2313 ◽  
Author(s):  
Kai Li ◽  
Che-Jen Lin ◽  
Wei Yuan ◽  
Guangyi Sun ◽  
Xuewu Fu ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Isotopic Composition ◽  
Stable Isotope Analysis ◽  
Natural Water ◽  
Water Samples ◽  
Isotope Analysis ◽  
New Method ◽  
Improved Method ◽  
Natural Water Samples

A new method is developed to meet the criteria for precise and efficient measurements of mercury isotopic composition in various water samples.

Improved Method of Data Processing for Flocculating Sedimentation Experiment

2012 ◽  
Vol 599 ◽  
pp. 340-343
Author(s):  
Yan Lin ◽  
Shu Wen Guo ◽  
Jing Hua Lin
Keyword(s):  
Data Processing ◽  
Removal Efficiency ◽  
Conventional Method ◽  
Present Method ◽  
New Method ◽  
Improved Method ◽  
Partial Removal

The shortages of the present method of data processing of flocculating sedimentation are pointed out and analyzed in this study. A new method of data processing is put forward and the advantages of this new method are discussed. Compared to the conventional method, the isolines of partial removal efficiency need not to be drawn when the new method is employed and the new method is straightforward, practical and accurate and it is worth to be popularized.

An Improved Method for Calculating the flow in Turbo-Machines, Including a Consistent loss Model

1974 ◽  
Vol 16 (1) ◽  
pp. 25-31 ◽  
Author(s):  
C. Bosman ◽  
H. Marsh
Keyword(s):  
Flow Theory ◽  
Computer Programs ◽  
Flow Patterns ◽  
New Method ◽  
Improved Method ◽  
Basic Principles ◽  
Loss Model ◽  
Through Flow ◽  
A Minor

The basic principles of the through-flow theory of turbomachines are described and an improved method of analysis is developed which includes a consistent loss model. The application of this new method requires only a minor modification to existing computer programs for calculating turbomachinery flow patterns.

Sign in / Sign up

Export Citation Format

Share Document