The transverse location of tryptophan residues in the purple membranes of Halobacterium halobium studied by fluorescence quenching and energy transfer

1984 ◽  
Vol 776 (1) ◽  
pp. 75-82 ◽  
Author(s):  
R.C. Chatelier ◽  
P.J. Rogers ◽  
K.P. Ghiggino ◽  
W.H. Sawyer
Keyword(s):  
Energy Transfer ◽  
Fluorescence Quenching ◽  
Halobacterium Halobium ◽  
Tryptophan Residues ◽  
Purple Membranes

The interaction between halogenated anaesthetics and bacteriorhodopsin in purple membranes as examined by intrinsic ultraviolet fluorescence

1991 ◽  
Vol 69 (2-3) ◽  
pp. 178-184 ◽  
Author(s):  
Ki-Hwan Lee ◽  
Alan R. McIntosh ◽  
François Boucher
Keyword(s):  
Energy Transfer ◽  
Spectral Properties ◽  
Direct Interaction ◽  
Halobacterium Halobium ◽  
Amino Acid Residues ◽  
Spectral Change ◽  
Structural Modifications ◽  
Halogenated Hydrocarbon ◽  
Purple Membranes ◽  
Rule Out

In the presence of halogenated general anaesthetics such as enflurane and halothane, the spectral properties of the bacteriorhodopsin pigment contained in the purple membranes of Halobacterium halobium are strongly modified. It is reversibly transformed into a red-coloured species absorbing maximally at 480 nm, at the expense of its characteristic 570-nm absorption band. The ultraviolet fluorescence of bacteriorhodopsin has been used to probe the structural modifications that are reflected by this spectral change. Our results show that they are very small and do not perturb the energy transfer dynamics which take place between the aromatic amino acid residues and the retinyl chromophore. The fluorescence properties of anaesthetic-treated bacteriorhodopsin are dominated by the quenching properties of the halogenated hydrocarbon, which are obvious even at anaesthetic concentrations under those needed to induce a spectral change in the bacteriorhodopsin chromophore. This does not rule out direct interaction between anaesthetics and bacteriorhodopsin, but it indicates that the chromophoric site might well not be their primary target.Key words: purple membranes, bacteriorhodopsin, anaesthetics, fluorescence, quenching.

scholarly journals Graphdiyne nanosheets as a platform for accurate copper(ii) ion detection via click chemistry and fluorescence resonance energy transfer

RSC Advances ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 5320-5324
Author(s):  
Chenchen Ge ◽  
Jiaofu Li ◽  
Dou Wang ◽  
Kongpeng Lv ◽  
Quan Liu ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Fluorescence Quenching ◽  
Click Chemistry ◽  
Fluorescence Resonance Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
High Specificity ◽  
Ion Detection ◽  
Fluorescence Resonance

Cu2+ detection was performed by taking advantage of the fluorescence quenching ability of graphdiyne and the high specificity of click chemistry.

A Unified Fluorescence Quenching Mechanism of Tetrazine-Based Dyes: Energy Transfer to a Dark State

2021 ◽  
Author(s):  
Weijie Chi ◽  
Lu Huang ◽  
Chao Wang ◽  
Davin Tan ◽  
Zhaochao Xu ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Fluorescence Quenching ◽  
Quenching Mechanism ◽  
Dark State ◽  
Medical Diagnostic ◽  
Fluorogenic Probes ◽  
Fluorescence Quenching Mechanism ◽  
Diagnostic Applications

Tetrazine-based fluorogenic probes are powerful tools for bioimaging, biosensing, and medical diagnostic applications. In these probes, the attachment of a tetrazine moiety generates a non-fluorescent precursor; upon the bio-orthogonal reaction...

Fluorescence energy transfer studies in a cross-linked polyurethane network

1995 ◽  
Vol 73 (11) ◽  
pp. 1823-1830 ◽  
Author(s):  
Jie Yang ◽  
Mitchell A. Winnik
Keyword(s):  
Energy Transfer ◽  
Fluorescence Quenching ◽  
Fluorescence Decay ◽  
Critical Distance ◽  
Nonradiative Energy Transfer ◽  
Fluorescence Energy Transfer ◽  
Distribution Analysis ◽  
Analysis Method ◽  
Acceptor Concentration ◽  
Fluorescence Energy

A series of cross-linked polyurethane samples, labeled with dyes suitable for fluorescence energy transfer experiments, were prepared (donor, phenanthrene; acceptor, anthracene). Fluorescence decay profiles for these samples were measured as a function of acceptor concentration. These decays obey Förster nonradiative energy transfer kinetics, with an energy transfer critical distance (R0) of 26.7 Å. Fluorescence intensities, calculated from the decays by integrating the decay profiles, also fit the Perrin model, with a quenching radius (Rs) of 25.6 Å. The fluorescence decay profiles were further examined with a distribution analysis method, which also revealed uniformly distributed donors and acceptors in the polymer matrices. Keywords: fluorescence quenching, fluorescence decay, phenanthrene, anthracene, polyurethane.

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