Protein synthesis directed by encephalomyocarditis virus RNA II. The in vitro synthesis of high molecular weight proteins and elements of the viral capsid

1971 ◽  
Vol 45 (3) ◽  
pp. 788-795 ◽  
Author(s):  
I. Biome ◽  
H. Aviv ◽  
P. Leder
Keyword(s):  
Molecular Weight ◽  
Protein Synthesis ◽  
High Molecular Weight ◽  
Encephalomyocarditis Virus ◽  
Viral Capsid ◽  
In Vitro Synthesis ◽  
Virus Rna ◽  
High Molecular Weight Proteins

In vitro synthesis of high molecular weight rubber by Hevea small rubber particles

2010 ◽  
Vol 109 (2) ◽  
pp. 107-114 ◽  
Author(s):  
Porntip Rojruthai ◽  
Jitladda Tangpakdee Sakdapipanich ◽  
Seiji Takahashi ◽  
Lee Hyegin ◽  
Motoyoshi Noike ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
In Vitro Synthesis ◽  
Rubber Particles

scholarly journals Generation of monoclonal antibodies detecting specific epitopes in locust antennae

1993 ◽  
Vol 175 (1) ◽  
pp. 45-57
Author(s):  
J. Strotmann ◽  
I. Boekhoff ◽  
S. Goggerle ◽  
H. Breer
Keyword(s):  
Molecular Weight ◽  
Monoclonal Antibody ◽  
Protein Synthesis ◽  
Monoclonal Antibodies ◽  
Embryonic Development ◽  
High Molecular Weight ◽  
Time Course ◽  
Antigen Expression

1. Following a tissue-specific screening paradigm, monoclonal antibodies have been generated that interact with distinct subpopulations of cells in locust antennae. 2. Antigens were identified as high molecular weight components. 3. Immunoreactivity was not detectable during embryonic development, but rapidly appeared within a few hours of hatching. 4. The time course of antigen expression in antennal cells could be followed in situ as well as in vitro. 5. Expression of monoclonal antibody B14/6D2-like immunoreactivity was prevented by blocking protein synthesis with cycloheximide.

scholarly journals MORPHOLOGICAL CHANGES AND THE REQUIREMENTS FOR MACROMOLECULE SYNTHESIS DURING EXCYSTMENT OF ACANTHAMOEBA CASTELLANII

1971 ◽  
Vol 49 (2) ◽  
pp. 507-519 ◽  
Author(s):  
Fathy E. Mattar ◽  
Thomas J. Byers
Keyword(s):  
Molecular Weight ◽  
Protein Synthesis ◽  
High Molecular Weight ◽  
Phase Contrast ◽  
Actinomycin D ◽  
Morphological Changes ◽  
Acanthamoeba Castellanii ◽  
Kinetics Of ◽  
Functional Rna

Light and phase-contrast microscopic observations of excystment in Acanthamoeba castellanii have been used to classify cells in excysting populations as free trophozoites, or mature, activated, or preemergent cysts. These categories have been used to describe the kinetics of excystment. A pH of 7 and a temperature of 30°C have been found to be optimal for the activation of mature cysts. Both activation and emergence are inhibited by cycloheximide and actinomycin D, but neither process is much affected by hydroxyurea. Cell-free extracts of high molecular weight components of cyst cytoplasm can support protein synthesis in vitro, although less efficiently than similar extracts from trophozoites. Evidence indicates that some of the functional RNA in the cyst extracts is synthesized before excystment.

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