Faculty Opinions recommendation of Peptide derived from HIV-1 TAT protein destabilizes a monolayer of endothelial cells in an in vitro model of the blood-brain barrier and allows permeation of high molecular weight proteins.

2012 ◽  
Author(s):  
Anne Moscona ◽  
Laura M Palermo ◽  
Matteo Porotto
Keyword(s):  
Molecular Weight ◽  
Endothelial Cells ◽  
High Molecular Weight ◽  
Blood Brain Barrier ◽  
In Vitro Model ◽  
Tat Protein ◽  
High Molecular Weight Proteins ◽  
Vitro Model ◽  
Hiv 1

Characterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC)

2016 ◽  
Vol 133 ◽  
pp. 100-112 ◽  
Author(s):  
Victor Llombart ◽  
Teresa García-Berrocoso ◽  
Joan Josep Bech-Serra ◽  
Alba Simats ◽  
Alejandro Bustamante ◽  
...  
Keyword(s):  
Cell Culture ◽  
Endothelial Cells ◽  
Stable Isotope ◽  
Blood Brain Barrier ◽  
Human Blood ◽  
In Vitro Model ◽  
Isotope Labeling ◽  
Vitro Model

Evidence of Mucin Secretion in Human Lung Adenocarcinoma Cell Lines NCIH650 and NCIH2077 and Effect of Select Secretagogues on Mucin Secretion

1999 ◽  
Vol 19 (5) ◽  
pp. 473-483 ◽  
Author(s):  
Prerna M. Sharma ◽  
Mangala G. Sarkar ◽  
Arvind K. Virmani ◽  
Adi F. Gazdar ◽  
Goverdhan P. Sachdev
Keyword(s):  
Molecular Weight ◽  
Lung Adenocarcinoma ◽  
High Molecular Weight ◽  
Cell Lines ◽  
In Vitro Model ◽  
Culture Conditions ◽  
Mucin Secretion ◽  
Adenocarcinoma Cell ◽  
Vitro Model

Mucins comprise an important class of tumor-associated antigens. The objectives of the present study were (a) to establish an in vitro model system using human non-small cell lung adenocarcinoma cell lines NCIH650 and NCIH2077 (b) provide evidence that these cell lines secrete mucin in culture conditions and (c) investigate the effects of select secretagogues on mucin secretion. The cell lines were established in ACL-4 medium containing several growth factors and retinoic acid and 5% fetal calf serum. The high molecular weight glycoconjugates secreted in the culture medium were purified by ammonium sulfate precipitation and Superose 6 and Superose 12 FPLC chromatography. The purified high molecular weight glycoconjugate fraction and the carcinoma cells were shown to have mucin by dot blot, Western blot and immunohistochemical analysis, respectively, using specific antibodies to purified major mucin, HTM-1. Also, incorporation experiments with mucin precursor 3H-glucosamine demonstrated that the cells indeed synthesize high molecular weight mucins. The effects of secretagogues such as, 8-bromocyclic AMP, ionomycin, phorbol-12-myristate-13-acetate and neutrophil elastase on mucin secretion were also investigated. Only 8-bromocyclic AMP and neutrophil elastase influenced mucin secretion. These studies provided strong evidence that the lung adenocarcinoma cell lines secrete high molecular weight mucins in culture conditions and only two of the four tested secretagogues significantly increased mucin secretion. Thus, this in vitro model system may be useful in determining alterations in mucin structure, if any, in lung adenocarcinomas as well as in studying the regulation of mucin gene expression.

Isolation and cultivation of porcine brain capillary endothelial cells as an in vitro model of the blood-brain barrier

1995 ◽  
Vol 17 (1) ◽  
pp. 25-32 ◽  
Author(s):  
D. J. Bobilya ◽  
K. D'Amour ◽  
A. Palmer ◽  
C. Skeffington ◽  
N. Therrien ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Blood Brain Barrier ◽  
In Vitro Model ◽  
Brain Barrier ◽  
Brain Capillary ◽  
Porcine Brain ◽  
Blood Brain ◽  
Capillary Endothelial Cells ◽  
Vitro Model
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