Changes in tyrosine hydroxylase and dopamine-beta-hydroxylase activities but not in phenylethanolamine-N-methyltransferase activity within central adrenaline neurons after 6-hydroxydopamine administration

1. Sinoaortic deafferentation in the rat leads to increased blood pressure and heart rate. 2. Early increases in tyrosine hydroxylase activity both in brain stem and hypothalamus suggest that increased noradrenaline synthesis may contribute to the development of neurogenic hypertension. 3. After 4 weeks, phenylethanolamine-N-methyltransferase activity was reduced in the hypothalamus. 4. Noradrenaline- and adrenaline, but not dopamine-containing neurones may participate in regulation of sympathetic efferent activity.

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Compensatory increase in tyrosine hydroxylase activity in rat brain after intraventricular injections of 6-hydroxydopamine

Science ◽

10.1126/science.6101509 ◽

1980 ◽

Vol 207 (4430) ◽

pp. 537-540 ◽

Cited By ~ 81

Author(s):

A. Acheson ◽

M. Zigmond ◽

E. Stricker

Keyword(s):

Tyrosine Hydroxylase ◽

Rat Brain ◽

Hydroxylase Activity ◽

Compensatory Increase ◽

Tyrosine Hydroxylase Activity ◽

Intraventricular Injections ◽

6 Hydroxydopamine

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Pituitary Adenylate Cyclase-Activating Polypeptide Induces Gene Expression of the Catecholamine Synthesizing Enzymes, Tyrosine Hydroxylase, and Dopamine beta-Hydroxylase in Cultured Porcine Adrenal Medullary Chromaffin Cells

Annals of the New York Academy of Sciences ◽

10.1111/j.1749-6632.1996.tb17504.x ◽

2006 ◽

Vol 805 (1) ◽

pp. 464-469 ◽

Cited By ~ 4

Author(s):

KAZUMASA ISOBE ◽

NOBUYASU YUKIMASA ◽

TOSHIAKI NAKAI ◽

YOH TAKUWA

Keyword(s):

Gene Expression ◽

Tyrosine Hydroxylase ◽

Adenylate Cyclase ◽

Chromaffin Cells ◽

Dopamine Beta Hydroxylase ◽

Pituitary Adenylate Cyclase ◽

Catecholamine Synthesizing Enzymes

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Cellular localization of tyrosine hydroxylase by immunohistochemistry.

Journal of Histochemistry & Cytochemistry ◽

10.1177/23.1.234988 ◽

1975 ◽

Vol 23 (1) ◽

pp. 1-12 ◽

Cited By ~ 186

Author(s):

V M Pickel ◽

T H Joh ◽

P M Field ◽

C G Becker ◽

D J Reis

Keyword(s):

Tyrosine Hydroxylase ◽

Dopaminergic Neurons ◽

Chromaffin Cells ◽

Cellular Localization ◽

Dopaminergic System ◽

Noradrenergic Neurons ◽

Dopamine Beta Hydroxylase ◽

The Central Nervous System ◽

Cervical Ganglia ◽

Large Neurons

The enzyme tyrosine hydroxylase (TH) was immunohistochemically localized by the peroxidase-antiperoxidase method in rat to chromaffin cells of the adrenal medulla, large neurons and small darkly staining cells of the superior cervical ganglia and noradrenergic and dopaminergic neurons in brain. As compared with the conjugated peroxidase or immunofluorescence techniques, the peroxidase-antiperoxidase method gave the most selective and specific cytoplasmic localization of TH antisera in every tissue examined. The peroxidase staining with the TH antisera was more intense in dopaminergic than in noradrenergic neurons of the central nervous system. While TH was visualized in cell bodies of both dopaminergic and noradrenergic neurons, it could only be detected in axons and terminals in the dopaminergic system. The perikarya of noradrenergic neurons could be distinguished from dopaminergic neurons by the immunohistochemical demonstration of the enzyme dopamine-beta-hydroxylase only in the former.

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