Endothelial cell growth supplement: a cell cloning factor that promotes the growth of monoclonal antibody producing hybridoma cells

1983 ◽  
Vol 61 (2) ◽  
pp. 195-200 ◽  
Author(s):  
Cristina Pintus ◽  
Janet H. Ransom ◽  
Charles H. Evans
Keyword(s):  
Monoclonal Antibody ◽  
Endothelial Cell ◽  
Cell Growth ◽  
Hybridoma Cells ◽  
Cell Cloning ◽  
Growth Supplement ◽  
Endothelial Cell Growth Supplement ◽  
A Cell ◽  
Endothelial Cell Growth

Effects of Heparin and Endothelial Cell Growth Supplement on Haemostatic Functions of Vascular Endothelium

1992 ◽  
Vol 67 (06) ◽  
pp. 718-723 ◽  
Author(s):  
Allison J Minter ◽  
Joan Dawes ◽  
Colin N Chesterman
Keyword(s):  
Endothelial Cell ◽  
Cell Growth ◽  
Plasminogen Activator ◽  
Plasminogen Activator Inhibitor ◽  
Growth Supplement ◽  
Endothelial Cell Growth Supplement ◽  
Endothelial Cell Growth ◽  
Von Willebrand ◽  
Willebrand Factor ◽  
Activator Inhibitor

SummaryHeparin in combination with endothelial cell growth factor (ECGF) affects physiological responses and growth of human umbilical vein endothelial cells (HUVEC). We have examined the effect of heparin, crude ECGF (endothelial cell growth supplement [ECGS]), or both on the basal and thrombin challenged output of metabolites by HUVEC. The supernatant and/or cell lysate was assayed for released prostacyclin, von Willebrand factor, tissue plasminogen activator, plasminogen activator inhibitor and thrombospondin. Heparin modified release of all these metabolites when in combination with ECGS, and in general these responses were the opposite of those generated by inflammatory mediators such as interleukin-1. It has been postulated that heparin acts by potentiating the effect of ECGF, but heparin inhibited thrombospondin release and enhanced that of von Willebrand factor in the absence of ECGS, while ECGS alone inhibited release of plasminogen activator inhibitor. Thus, under our experimental conditions it would appear that heparin and crude ECGF can affect HUVEC independently of one another.

A rapid and simple isolation of endothelial cell growth supplement from mouse brain

1995 ◽  
Vol 79 (1) ◽  
pp. 141-145
Author(s):  
Şükran Şahin (Özgün) ◽  
Tevfik Akoǧlu ◽  
Roger Lawrence ◽  
Leyla Semiha Şen
Keyword(s):  
Endothelial Cell ◽  
Cell Growth ◽  
Mouse Brain ◽  
Growth Supplement ◽  
Endothelial Cell Growth Supplement ◽  
Endothelial Cell Growth
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