Chemical characterization of a prominent phosphomonoester resonance from mammalian brain. 31P and 1H NMR analysis at 4.7 and 14.1 tesla

Objective: Present study was aimed to isolate and chemically characterize bioactive constituent from alkaloid enriched fraction, prepared from roots of Argyreia speciosa Linn. Materials and methods: Literature review revealed presence of ergoline type of alkaloids in roots. Alkaloidal fraction was prepared and screened for its action on testosterone biosynthesis, in- vitro, using isolated rat leydig cells. Dehydroepiandosterone was used as positive control. This bioactive fraction was subjected to open column chromatography followed by flash chromatography, to isolate constituent. One compound (A1) was isolated from the fraction and its purity was ascertained using TLC and HPLC studies. A1 was chemically characterized by IR, Mass and 1H-NMR studies, to elucidate probable chemical structure. A1 was screened for action on testosterone synthesis too, using isolated rat Leydig cells model. The fraction was standardized with respect to amount of A1 present. Results: Alkaloidal fraction (1000 µg/ml) incubated Leydig cells showed nearly, 22 fold increase in testosterone content as compared to untreated cells. The studies also yielded increase in testosterone content, in cells treated with test fractions and as observed in case of positive control. TLC studies indicated that A1 might possess ergoline moiety in the structure. IR, Mass and 1H NMR spectral studies suggested that A1 might be N-methyl ergometrine. This was the first report included isolation and chemical characterization of N-methyl ergometrine from Argyreia speciosa. A1 (1000 µg/ml) was found to stimulate testosterone content, by 14.62 fold, in culture media of Leydig cells after incubation. Conclusion: The results of in vitro studies, confirmed that the standardized alkaloid fraction as well as A1 had ability to stimulate Leydig cells to secrete testosterone. A1 might be N-methyl ergometrine and being ergometrine derivative it might act through oxytocine receptors expressed on the Leydig cells and stimulates testosterone synthesis.

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Revealing the chemical characterization of asphaltenes fractions produced by N-methylpyrrolidone using FTIR, molecular fluorescence, 1H NMR, and ESI(±)FT-ICR MS

Fuel ◽

10.1016/j.fuel.2017.08.098 ◽

2017 ◽

Vol 210 ◽

pp. 514-526 ◽

Cited By ~ 20

Author(s):

Verônica V. Carvalho ◽

Géssica A. Vasconcelos ◽

Lilian V. Tose ◽

Heloa Santos ◽

Felipe M.R. Cardoso ◽

...

Keyword(s):

1H Nmr ◽

Chemical Characterization ◽

Molecular Fluorescence ◽

Ft Icr Ms ◽

Ft Icr

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Synthesis and Characterization of Cholesterol-(1,2,3-triazole)-PEG via Click Chemistry

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.647.499 ◽

2013 ◽

Vol 647 ◽

pp. 499-503 ◽

Cited By ~ 3

Author(s):

Wan Xu ◽

Bing Hong Luo ◽

Cai Rong Li ◽

Jing Yang ◽

Chang Ren Zhou

Keyword(s):

Polyethylene Glycol ◽

Click Chemistry ◽

1H Nmr ◽

Sodium Azide ◽

Synthesis And Characterization ◽

Nmr Analysis

Firstly, toluenesulfonyl cholesterol was synthesized by the reaction of cholesterol and p-toluenesulfonyl chloride, and then reacted with sodium azide to obtain azido-cholesterol. Secondly, propargyl was introduced into the terminal groups of polyethylene glycol (PEG) by the reaction of PEG and bromine propargyl. Lastly, cholesterol-(1,2,3-triazole)-PEG oligomer was prepared by the reaction of the azido-cholesterol and propargyl-PEG via click chemistry using CuSO4.5H2O as a catalyst. The structures of the products were characterized by the FTIR and 1H NMR analysis.

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Fractionation and characterization of sludge bacterial extracellular polymers by FT-IR, 13C-NMR, 1H-NMR

Water Science & Technology ◽

10.2166/wst.2001.0584 ◽

2001 ◽

Vol 44 (10) ◽

pp. 71-78 ◽

Cited By ~ 8

Author(s):

L.X. Zhou ◽

J.R. Liang ◽

X.H. Zhan ◽

J.W.C. Wong

Keyword(s):

Activated Sludge ◽

1H Nmr ◽

13C Nmr ◽

Chemical Characterization ◽

Acid Base ◽

Extracellular Polymers ◽

Hydrophilic Fraction ◽

Aromatic Substance ◽

Ft Ir

Sludge bacterial extracellular (BEP) polymers exhibit a strong affinity with metals in addition to their role in the bioflocculation of activated sludge. It is of utmost importance in elucidating the chemical characterization of sludge BEP prior to a better understanding of its environmental behavior. Sludge BEP extracted from activated sludge was characterized by FT-IR, 1H-NMR, and 13C-NMR and fractionated into 6 fractions (hydrophilic acid, base, and neutral; and hydrophobic acid, base, and neutral). The results indicated that sludge BEP contained more polysaccharides but fewer amino acids or proteinaceous materials compared to dissolved organic matter (DOM) derived from sludge. No aromatic substance was found in sludge BEP as indicated by NMR. Sludge BEP was dominated by hydrophilic fractions, accounting for 65% of total DOC of sludge BEP and hydrophilic neutral constituted about 58% of the hydrophilic fraction. The chemical fraction and characterization indicated that sludge BEP might exhibit a higher capability in enhancing the transport of the associated contaminants through porous media than that of sludge DOM.

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Olfactory and Chemical Characterization of Indoor Air-Towards a Psychophysical Model for Air Quality

PsycEXTRA Dataset ◽

10.1037/e612282010-001 ◽

1981 ◽

Author(s):

Birgitta Berglund ◽

Ulf Berglund ◽

Thomas Lindvall ◽

Helene Nicander-Bredberg

Keyword(s):

Air Quality ◽

Indoor Air ◽

Chemical Characterization

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The physical and chemical characterization of active carbons, by adsorption and immersion techniques

Journal de Chimie Physique ◽

10.1051/jcp/1984810785 ◽

1984 ◽

Vol 81 ◽

pp. 785-790 ◽

Cited By ~ 9

Author(s):

F. Stoeckli ◽

F. Kraehenbuehl ◽

A. Lavanchy ◽

U. Huber

Keyword(s):

Chemical Characterization ◽

Active Carbons ◽

Physical And Chemical Characterization ◽

Physical And Chemical

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Biological and physico-chemical characterization of ultra high diluted Hydrastis canadensis and in vitro studies on mammalian cells

Allgemeine Homöopathische Zeitung ◽

10.1055/s-0037-1601241 ◽

2017 ◽

Vol 262 (02) ◽

pp. 2-76

Author(s):

N Chandrakar ◽

MK Temgire ◽

SG Kane ◽

AK Suresh ◽

J Bellare

Keyword(s):

Mammalian Cells ◽

Chemical Characterization ◽

In Vitro Studies ◽

Hydrastis Canadensis ◽

Physico Chemical

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PURIFICATION AND PARTIAL CHEMICAL CHARACTERIZATION OF SHEEP NEUROPHYSIN

Acta Endocrinologica ◽

10.1530/acta.0.0740226 ◽

1973 ◽

Vol 74 (2) ◽

pp. 226-236 ◽

Cited By ~ 6

Author(s):

Michel Chrétien ◽

Claude Gilardeau

Keyword(s):

Amino Acid ◽

Polyacrylamide Gel Electrophoresis ◽

Chemical Characterization ◽

Terminal Amino Acid ◽

Amino Acid Determination ◽

Pituitary Glands ◽

Terminal Amino ◽

Partial Chemical ◽

Acid Determination

ABSTRACT A protein isolated from ovine pituitary glands has been purified, and its homogeneity assessed by NH2- and COOH-terminal amino acid determination, ultracentrifugation studies, and polyacrylamide gel electrophoresis after carboxymethylation. Its chemical and immunochemical properties are closely similar to those of beef and pork neurophysins, less similar to those of human neurophysins. It contains no tryptophan (like other neurophysins) or histidine (like all except bovine neurophysin-I and human neurophysins). It has alanine at the NH2-terminus and valine at the COOH-terminus. Its amino acid composition is similar to, but not identical with those of porcine and bovine neurophysins.

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