Diabetes-induced bile acid composition changes in rat bile determined by high performance liquid chromatography

Life Sciences ◽  
1991 ◽  
Vol 49 (18) ◽  
pp. 1301-1308 ◽  
Author(s):  
Yong Siow ◽  
Avital Schurr ◽  
Gary C Vitale
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Bile Acid ◽  
Acid Composition ◽  
High Performance ◽  
Composition Changes ◽  
Rat Bile

Differential intestinal deconjugation of taurine and glycine bile acid N-acyl amidates in rats

1992 ◽  
Vol 262 (2) ◽  
pp. G351-G358
Author(s):  
R. Zhang ◽  
S. Barnes ◽  
R. B. Diasio
Keyword(s):  
High Performance Liquid Chromatography ◽  
Small Intestine ◽  
Liquid Chromatography ◽  
Bile Acid ◽  
Chenodeoxycholic Acid ◽  
High Performance ◽  
Biliary Fistula ◽  
The Difference ◽  
The Stability ◽  
Rat Bile

Mechanisms responsible for the difference in the relative amounts of taurine- and glycine-conjugated bile acid N-acyl amidates (Tau/Gly ratio) are not fully understood. In the present study, the stability of taurine- and glycine-conjugated bile acid N-acyl amidates during intestinal transit and absorption was examined to investigate the contribution of intestinal deconjugation to the Tau/Gly ratio in rat bile. Radiolabeled chenodeoxycholic acid (CDC) and its N-acyl amidates with glycine (CDC-Gly) or taurine (CDC-Tau) were introduced into the lumen of the upper small intestine in the biliary fistula rats, and radioactive metabolites in bile, blood, urine, and tissues were identified and quantitated by high-performance liquid chromatography. Results indicated that 1) extensive deconjugation of CDC-Gly occurs during intestinal absorption; 2) CDC-Tau is recovered in bile largely intact; and 3) newly synthesized CDC-Tau and CDC-Gly are formed in a ratio of less than 2:1 after administration of [14C]-CDC. In summary, the present study demonstrates that resistance of taurine-conjugated bile acid N-acyl amidates to hydrolysis in the intestine, rather than a difference in synthesis of taurine- and glycine-conjugated N-acyl amidates in liver, may account for the high Tau/Gly ratio in rat bile.

Determination of bile acids in rat bile by high-performance liquid chromatography

1985 ◽  
Vol 338 ◽  
pp. 195-200 ◽  
Author(s):  
Morimasa Hayashi ◽  
Yasuharu Imai ◽  
Yuzo Minami ◽  
Sumio Kawata ◽  
Yuji Matsuzawa ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Bile Acids ◽  
High Performance ◽  
Rat Bile

Analysis of the fatty acid composition of human serum lipids by high performance liquid chromatography

1984 ◽  
Vol 143 (3) ◽  
pp. 361-366 ◽  
Author(s):  
Yoshiharu Shimomura ◽  
Kiyoaki Taniguchi ◽  
Toshiharu Sugie ◽  
Masayuki Murakami ◽  
Satoru Sugiyama ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Fatty Acid Composition ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Human Serum ◽  
Acid Composition ◽  
High Performance ◽  
Serum Lipids

scholarly journals Determination of Amino Acid Composition in the Harpagophytum procumbens Root

2019 ◽  
Vol 18 (1) ◽  
pp. 85-91
Author(s):  
AI Kriukova ◽  
IM Vladymyrova ◽  
OL Levashova ◽  
TS Tishakova
Keyword(s):  
Amino Acids ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
High Performance ◽  
Harpagophytum Procumbens

The amino acid composition of the roots of Harpagophytum procumbens was investigated by the method of high performance liquid chromatography (HPLC) with preliminary derivatization. Sixteen free and thirteen bound amino acids were quantitatively determined. The content of protein-bound amino acids was calculated. Dhaka Univ. J. Pharm. Sci. 18(1): 85-91, 2019 (June)

Separation of keto and keto-hydroxy bile acid isomers by high-performance liquid chromatography

1988 ◽  
Vol 438 ◽  
pp. 423-428 ◽  
Author(s):  
Takashi Iida ◽  
Toshiyuki Shinohara ◽  
Toshiaki Momose ◽  
Frederic C. Chang ◽  
Junichi Goto ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Bile Acid ◽  
High Performance
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