Photochemical analysis studies—III A fluorimetric and ultraviolet spectrophotometric study of the photolysis of chloroquine on silica-gel thin layers, and its analytical application

Talanta ◽  
1982 ◽  
Vol 29 (5) ◽  
pp. 383-389 ◽  
Author(s):  
J Aaron
Keyword(s):  
Silica Gel ◽  
Spectrophotometric Study ◽  
Thin Layers ◽  
Analytical Application

scholarly journals Chemical synthesis of all-trans-β-retinoyl phosphate

1976 ◽  
Vol 159 (3) ◽  
pp. 799-801 ◽  
Author(s):  
J P Frot-Coutaz ◽  
L M de Luca
Keyword(s):  
Retinoic Acid ◽  
Chemical Synthesis ◽  
Cellulose Acetate ◽  
Silica Gel ◽  
Absorption Maximum ◽  
Ammonium Acetate ◽  
Deae Cellulose ◽  
Thin Layers ◽  
Molar Ratio ◽  
Chloroform Methanol

all-trans-β-Retinoic acid is phosphorylated to retinoyl phosphate by bis(triethylamine) phosphate with yields of 10-15%. The product is soluble in methanol and is eluted from DEAE-cellulose acetate at a concentration of 0.1M-ammonium acetate in 99% (v/v) methanol. Its phosphate/retinoic acid molar ratio is 1. Retinoyl phosphate has an absorption maximum at 360nm in methanol, whereas retinoic acid has a maximum at 350 nm. The compound is hydrolysed at pH2 and pH13 for 20 min at 37 degrees C, but is relatively stable under the same conditions at pH4, 6, 8 and 10. Retinoyl phosphate (RF 0.1) can be separated from retinyl phosphate (RF 0.2) by chromatography on thin layers of silica gel in chloroform/methanol/water (60:25:4, by vol.).

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