Toxic skin secretions of the spade-foot toad Pelobates fuscus

Since nowadays the chemical synthesis of new bioactive compounds is a complicated and expensive procedure, alongside with the increased price for drugs based on synthetic biologically active compounds, scientists lay emphasize on natural sources as a promising raw material for various biologically active substances. Amphibian skin glands secretions are a powerful source of potential pharmacological agents. Currently their antimicrobial, antiviral, cardiotonic and antidiabetic activities have been studied in detail, although almost nothing is known about the effects of the tailless amphibians' skin secretions on the functioning of the hemostasis system. The aim of this study was to analyze the potential effects of the components of skin glands secretions of a representative of Ukrainian batrakhofauna – the Common spadefoot toad (Pelobates fuscus) on some parameters of hemostasis system. In the result of chromatographic separation of general skin secretions, 4 protein fractions were obtained, containing a variety of proteins with molecular masses ranging from 17 to 150 kDa. The components of fraction 1 activated prothrombin and factor X in plasma. Several components of fraction 4 had proteolytic activity and substrate specificity for collagen. The components of fractions 1 and 2 prolonged plasma coagulation time in the APTT. Thus, it was proved the presence of biologically active compounds in the P. fuscus skin secretions, that indicates the prospects for further research to identify the individual components responsible for the manifestation of the shown effects in order to provide clues in understanding the structural and functional characteristics and mechanism of action. Moreover, advances in this area will further increase the use of amphibian skin secretions as a raw material for the development of new original pharmaceutical agents and/or biotechnological products

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Correction to: Predation of treefrogs (Anura: Hylidae) with toxic skin secretions by the black lion tamarin (Leontopithecus chrysopygus, Callitrichinae)

Primates ◽

10.1007/s10329-020-00827-0 ◽

2020 ◽

Vol 61 (4) ◽

pp. 573-573

Author(s):

Guilherme S. T. Garbino ◽

Leonardo Henrique da Silva ◽

Rodrigo Gonçalves Amaral ◽

Gabriela Cabral Rezende ◽

Vinicius J. A. Pereira ◽

...

Keyword(s):

Lion Tamarin ◽

Skin Secretions ◽

Toxic Skin

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Predation of treefrogs (Anura: Hylidae) with toxic skin secretions by the black lion tamarin (Leontopithecus chrysopygus, Callitrichinae)

Primates ◽

10.1007/s10329-020-00818-1 ◽

2020 ◽

Vol 61 (4) ◽

pp. 567-572

Author(s):

Guilherme S. T. Garbino ◽

Leonardo Henrique da Silva ◽

Rodrigo Gonçalves Amaral ◽

Gabriela Cabral Rezende ◽

Vinicius J. A. Pereira ◽

...

Keyword(s):

Lion Tamarin ◽

Skin Secretions ◽

Toxic Skin

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Bufadienolides from the Skin Secretions of the Neotropical Toad Rhinella alata (Anura: Bufonidae): Antiprotozoal Activity against Trypanosoma cruzi

Molecules ◽

10.3390/molecules26144217 ◽

2021 ◽

Vol 26 (14) ◽

pp. 4217

Author(s):

Candelario Rodriguez ◽

Roberto Ibáñez ◽

Luis Mojica ◽

Michelle Ng ◽

Carmenza Spadafora ◽

...

Keyword(s):

Vero Cells ◽

Chemical Diversity ◽

Hydroxyl Group ◽

Antitrypanosomal Activity ◽

The Family ◽

Toad Venom ◽

Skin Secretions ◽

Pharmacological Potential ◽

First Time ◽

Mcf 7

Toads in the family Bufonidae contain bufadienolides in their venom, which are characterized by their chemical diversity and high pharmacological potential. American trypanosomiasis is a neglected disease that affects an estimated 8 million people in tropical and subtropical countries. In this research, we investigated the chemical composition and antitrypanosomal activity of toad venom from Rhinella alata collected in Panama. Structural determination using mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy led to the identification of 10 bufadienolides. Compounds identified include the following: 16β-hydroxy-desacetyl-bufotalin-3-adipoyl-arginine ester (1), bufotalin (2), 16β-hydroxy-desacetyl-bufotalin-3-pimeloyl-arginine ester (3), bufotalin-3-pimeloyl-arginine ester (4), 16β-hydroxy-desacetyl-bufotalin-3-suberoyl-arginine ester (5), bufotalin-3-suberoyl-arginine ester (6), cinobufagin-3-adipoyl-arginine ester (7), cinobufagin-3-pimeloyl-arginine ester (8), cinobufagin-3-suberoyl-arginine ester (9), and cinobufagin (10). Among these, three new natural products, 1, 3, and 5, are described, and compounds 1–10 are reported for the first time in R. alata. The antitrypanosomal activity assessed in this study revealed that the presence of an arginyl-diacid attached to C-3, and a hydroxyl group at C-14 in the structure of bufadienolides that is important for their biological activity. Bufadienolides showed cytotoxic activity against epithelial kidney Vero cells; however, bufagins (2 and 10) displayed low mammalian cytotoxicity. Compounds 2 and 10 showed activity against the cancer cell lines MCF-7, NCI-H460, and SF-268.

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Toxic skin eruption induced by fludarabine

European Journal of Internal Medicine ◽

10.1016/s0953-6205(02)00217-0 ◽

2003 ◽

Vol 14 (2) ◽

pp. 134-135

Author(s):

Brigitte Granel ◽

Jacques Serratrice ◽

Nicoleta Ene ◽

Marie-Christine Rojat-Habib ◽

Réda Bouabdallah ◽

...

Keyword(s):

Skin Eruption ◽

Toxic Skin

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Unravelling the Skin Secretion Peptides of the Gliding Leaf Frog, Agalychnis spurrelli (Hylidae)

Biomolecules ◽

10.3390/biom9110667 ◽

2019 ◽

Vol 9 (11) ◽

pp. 667 ◽

Cited By ~ 3

Author(s):

Carolina Proaño-Bolaños ◽

Ailín Blasco-Zúñiga ◽

José Rafael Almeida ◽

Lei Wang ◽

Miguel Angel Llumiquinga ◽

...

Keyword(s):

Inhibitory Concentration ◽

Structural Diversity ◽

Biologically Active ◽

Biological Characterization ◽

Skin Secretion ◽

Peptide Profile ◽

Active Chemical ◽

Molecular Masses ◽

Skin Secretions

Frog skin secretions contain medically-valuable molecules, which are useful for the discovery of new biopharmaceuticals. The peptide profile of the skin secretion of Agalychnis spurrelli has not been investigated; therefore, the structural and biological characterization of its compounds signify an inestimable opportunity to acquire new biologically-active chemical scaffolds. In this work, skin secretion from this amphibian was analysed by molecular cloning and tandem mass spectrometry. Although the extent of this work was not exhaustive, eleven skin secretion peptides belonging to five peptide families were identified. Among these, we report the occurrence of two phyllokinins, and one medusin-SP which were previously reported in other related species. In addition, eight novel peptides were identified, including four dermaseptins, DRS-SP2 to DRS-SP5, one phylloseptin-SP1, and three orphan peptides. Phylloseptin-SP1 and dermaseptins-SP2 were identified in HPLC fractions based on their molecular masses determined by MALDI-TOF MS. Among the antimicrobial peptides, dermaseptin-SP2 was the most potent, inhibiting Escherichia coli, Staphylococcus aureus, and ORSA with a minimum inhibitory concentration (MIC) of 2.68 μM, and Candida albicans with an MIC of 10.71 μM, without haemolytic effects. The peptides described in this study represent but a superficial glance at the considerable structural diversity of bioactive peptides produced in the skin secretion of A. spurrelli.

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