Bovine serum albumin humoral immune response in aged fischer 344 rats

1981 ◽  
Vol 16 (1) ◽  
pp. 61-66 ◽  
Author(s):  
Phyllis-Jean Kaplan ◽  
Thomas J. Caperna ◽  
Justine S. Garvey
Keyword(s):  
Immune Response ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Humoral Immune Response ◽  
Bovine Serum ◽  
Fischer 344 Rats ◽  
Humoral Immune ◽  
Fischer 344

Rhamnose modified bovine serum albumin as a carrier protein promotes the immune response against sTn antigen

2020 ◽  
Vol 56 (90) ◽  
pp. 13959-13962
Author(s):  
Han Lin ◽  
Haofei Hong ◽  
Jinfeng Wang ◽  
Chen Li ◽  
Zhifang Zhou ◽  
...  
Keyword(s):  
Immune Response ◽  
Bovine Serum Albumin ◽  
Immune Responses ◽  
Serum Albumin ◽  
Cancer Vaccine ◽  
Bovine Serum ◽  
Vaccine Development ◽  
Carrier Protein ◽  
Antigen Uptake ◽  
Specific Antibodies

Rhamnose and sTn antigen were co-conjugated to bovine serum albumin (BSA) for cancer vaccine development. The immune responses against sTn have been significantly augmented with the involvement of Rha-specific antibodies to enhance antigen uptake.

scholarly journals THE NATURE OF ANTIGEN-ANTIBODY COMPLEXES FORMED IN RABBITS DURING AN IMMUNE RESPONSE TO BOVINE SERUM ALBUMIN

1958 ◽  
Vol 107 (5) ◽  
pp. 653-663 ◽  
Author(s):  
William O. Weigle
Keyword(s):  
Immune Response ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Ammonium Sulfate ◽  
Bovine Serum ◽  
Ammonium Sulfate Precipitation ◽  
Antibody Synthesis ◽  
Circulating Antigen ◽  
Antigen Antibody

The immune elimination of soluble BSA, following an intravenous injection, is accompanied by the appearance of circulating antigen-antibody complexes. The pattern of the appearance of circulating antigen-antibody complexes and the immune elimination of antigen probably depends on the amount of antigen injected, the rate of antibody synthesis, and perhaps, the quality of antibody produced. There is no relationship between the I* antigen-antibody complexes detected during the immune response in rabbits by ammonium sulfate precipitation and the material precipitated from immune sera as a result of treatment with alkali. Alkali-precipitable material present in the serum of rabbits at a time when I* antigen is also present contain at most only traces of the antigen.

scholarly journals Allogeneic Mesenchymal Stem Cell Treatment Induces Specific Alloantibodies in Horses

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Sean D. Owens ◽  
Amir Kol ◽  
Naomi J. Walker ◽  
Dori L. Borjesson
Keyword(s):  
Bone Marrow ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Research Projects ◽  
Allogeneic Mscs ◽  
Humoral Immune ◽  
Flow Cytometric ◽  
Stem Cell Treatment ◽  
Mesenchymal Stem Cell Treatment

Background.It is unknown whether horses that receive allogeneic mesenchymal stem cells (MSCs) injections develop specific humoral immune response. Our goal was to develop and validate a flow cytometric MSC crossmatch procedure and to determine if horses that received allogeneic MSCs in a clinical setting developed measurable antibodies following MSC administration.Methods.Serum was collected from a total of 19 horses enrolled in 3 different research projects. Horses in the 3 studies all received unmatched allogeneic MSCs. Bone marrow (BM) or adipose tissue derived MSCs (ad-MSCs) were administered via intravenous, intra-arterial, intratendon, or intraocular routes. Anti-MSCs and anti-bovine serum albumin antibodies were detected via flow cytometry and ELISA, respectively.Results.Overall, anti-MSC antibodies were detected in 37% of the horses. The majority of horses (89%) were positive for anti-bovine serum albumin (BSA) antibodies prior to and after MSC injection. Finally, there was no correlation between the amount of anti-BSA antibody and the development of anti-MSC antibodies.Conclusion.Anti allo-MSC antibody development was common; however, the significance of these antibodies is unknown. There was no correlation between either the presence or absence of antibodies and the percent antibody binding to MSCs and any adverse reaction to a MSC injection.

scholarly journals CELLULAR EVENTS IN THE IMMUNE RESPONSE

1968 ◽  
Vol 128 (4) ◽  
pp. 681-698 ◽  
Author(s):  
Donald J. Raidt ◽  
R. I. Mishell ◽  
Richard W. Dutton
Keyword(s):  
Immune Response ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Spleen Cell ◽  
Density Gradient ◽  
Bovine Serum ◽  
Plaque Assay ◽  
Precursor Cells ◽  
Cell Suspensions ◽  
Cellular Events

Cell suspensions from the spleens of normal mice or mice injected with sheep erythrocytes were separated on a discontinous bovine serum albumin density gradient. Four bands or subpopulations were obtained and were assayed for antibody-forming cells, and for antigen-sensitive precursor cells. The antibody-forming cells were assayed by the hemolytic plaque assay and the antigen-sensitive precursors were assayed by the number of plaque-forming cells which developed after 3 or 5 day's culture with antigen. It was found that both antibody-forming cells and their precursors were present in the denser region of the gradient when spleen cell suspensions were taken from unimmunized mice. In contrast, both antibody-forming cells and precursors floated to the top in cell suspensions from mice sacrificed 1, 2, or 3 days after antigen injection. The change in density was detectable as early as 12 hr and was complete by 18 hr. The cell which changed in density was specific for the antigen that brought about that change. The significance of these findings is discussed.

scholarly journals Milk and Meat Allergens from Bos taurus β-Lactoglobulin, α-Casein, and Bovine Serum Albumin: An In-Vivo Study of the Immune Response in Mice

Nutrients ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 2095 ◽  
Author(s):  
Ewa Fuc ◽  
Dagmara Złotkowska ◽  
Barbara Wróblewska
Keyword(s):  
Immune Response ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Cellular Response ◽  
Cow Milk ◽  
Mice Model ◽  
Beef Meat ◽  
Β Lactoglobulin

The mechanism of food allergy may vary. This study aimed to compare the effects of milk, yogurt, or beef meat supplementation on humoral and cellular immune responses in a mice model. Mice were divided into four groups: The “Milk group” was sensitized with a β-lactoglobulin (β-lg)/α-casein (α-CN) mixture and supplemented cow milk; the “Yogurt group” was sensitized with β-lg/α-CN and supplemented yogurt; the “Beef group” was immunized with bovine serum albumin (BSA) and supplemented beef meat; and the “PBS group” received PBS in all procedures. ELISA was used to measure humoral response, including: Total IgE, specific IgG, and IgA. Cellular response was determined by phenotyping lymphocyte from lymphoid tissue and measuring the Th1/Th2 cytokine concentration with flow cytometry. The qPCR method was used for quantification of the fecal microbiota. The results obtained revealed a lower IgE level for the Yogurt group than for the Milk one. In the Yogurt group, the contribution of regulatory T cells to MLN and PP was higher compared to the other groups. We confirmed that diet supplementation with yogurt modulates the immune response to the prime allergen, and changes the activity of serum antibodies to milk proteins and BSA. Based on a specific antibodies level, we cannot exclude the possibility of CMA mice reaction against BSA.

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